Date published: 2025-9-7

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KCTD12 抗体 (F-6): sc-271855

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说明书
  • KCTD12 抗体 F-6 是小鼠单克隆 IgG1 (kappa轻链),KCTD12抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的KCTD12的内部区域内的氨基酸117-137之间
  • KCTD12 抗体 (F-6) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 KCTD12; 也和以下物种反应,包括: canine
  • 抗KCTD12抗体(F-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取KCTD12 (F-6): sc-271855免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是KCTD12 Antibody (F-6) for WB applications. 的首选辅助检测试剂,这些试剂现与KCTD12 Antibody (F-6) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

KCTD12抗体(F-6)是一种IgG1 κ小鼠单克隆KCTD12抗体,可通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的KCTD12蛋白。KCTD12抗体(F-6)既有非偶联型抗KCTD12抗体形式,也有多种偶联型抗KCTD12抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。BTB(Broad-Complex、Tramtrack和Bric a brac)结构域,也称为POZ(POxvirus和锌指)结构域,是一个N端同二聚化结构域,包含多个kelch重复序列和/或C2H2型锌指。含有BTB结构域的蛋白质被认为通过控制染色质结构和功能参与转录调控。KCTD12(含钾通道四聚化结构域12),也称为PFET1或PFETIN,是一种由325个氨基酸组成的蛋白质,在胎儿器官中表达,在耳蜗和大脑中表达水平最高,在成人器官(如大脑和肺)中表达水平极低。KCTD12被认为是胃肠道间质瘤的预后生物标志物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

KCTD12 抗体 (F-6) 参考文献:

  1. 从耳蜗中分离出一种新型人类无内含子基因,该基因主要在胎儿期表达。  |  Resendes, BL., et al. 2004. J Assoc Res Otolaryngol. 5: 185-202. PMID: 15357420
  2. 利用实时 RT-PCR 技术从不同骨骼中筛选骨髓基质细胞的共同标记物:通过数和供体年龄的影响。  |  Igarashi, A., et al. 2007. Tissue Eng. 13: 2405-17. PMID: 17596118
  3. 蛋白质组学揭示胃肠道间质瘤的预后生物标志物--Pfetin。  |  Suehara, Y., et al. 2008. Clin Cancer Res. 14: 1707-17. PMID: 18347171
  4. 含有 BTB 结构域的人类 KCTD1 基因的特征和表达,该基因介导转录抑制和同源相互作用。  |  Ding, XF., et al. 2008. DNA Cell Biol. 27: 257-65. PMID: 18358072
  5. 骨与软组织肉瘤的全球蛋白质表达分析。  |  Kawai, A., et al. 2008. Clin Orthop Relat Res. 466: 2099-106. PMID: 18535868
  6. BTB 结构域主要存在于锌指蛋白中,它定义了一个进化保守的家族,其中包括果蝇的几个发育调控基因。  |  Zollman, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 10717-21. PMID: 7938017
  7. POZ 结构域:一种保守的蛋白质-蛋白质相互作用结构域。  |  Bardwell, VJ. and Treisman, R. 1994. Genes Dev. 8: 1664-77. PMID: 7958847
  8. PLZF BTB 结构域的晶体结构。  |  Ahmad, KF., et al. 1998. Proc Natl Acad Sci U S A. 95: 12123-8. PMID: 9770450

订购信息

产品名称产品编号规格价格数量收藏夹

KCTD12 抗体 (F-6)

sc-271855
200 µg/ml
$316.00

KCTD12 (F-6): m-IgG Fc BP-HRP 套装

sc-527301
200 µg Ab; 10 µg BP
$354.00

KCTD12 (F-6): m-IgG1 BP-HRP 套装

sc-532674
200 µg Ab; 20 µg BP
$354.00

KCTD12 抗体 (F-6) AC

sc-271855 AC
500 µg/ml, 25% agarose
$416.00

KCTD12 抗体 (F-6) HRP

sc-271855 HRP
200 µg/ml
$316.00

KCTD12 抗体 (F-6) FITC

sc-271855 FITC
200 µg/ml
$330.00

KCTD12 抗体 (F-6) PE

sc-271855 PE
200 µg/ml
$343.00

KCTD12 抗体 (F-6) Alexa Fluor® 488

sc-271855 AF488
200 µg/ml
$357.00

KCTD12 抗体 (F-6) Alexa Fluor® 546

sc-271855 AF546
200 µg/ml
$357.00

KCTD12 抗体 (F-6) Alexa Fluor® 594

sc-271855 AF594
200 µg/ml
$357.00

KCTD12 抗体 (F-6) Alexa Fluor® 647

sc-271855 AF647
200 µg/ml
$357.00

KCTD12 抗体 (F-6) Alexa Fluor® 680

sc-271855 AF680
200 µg/ml
$357.00

KCTD12 抗体 (F-6) Alexa Fluor® 790

sc-271855 AF790
200 µg/ml
$357.00

KCTD12 (F-6) 中和勝肽

sc-271855 P
100 µg/0.5 ml
$68.00

Could you explain to me why the Western Blot analysis shows what appears to be a doublet? I can see it in the datasheet images and in my blots but I would like to know the reason. Thank you!

Asked by: ev0292
Thank you for your question. The predicted molecular weight for KCTD12 is approximately 36 kDa. The appearance of a doublet may be the result of some post-translational modification of the protein, such as phosphorylation.
Answered by: Technical Support
Date published: 2019-08-17

What application is the blocking peptide sc-271855 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Buon funzionamentoLa banda è flebile ma chiara!! Si può utilizzare. L'ho testato sulle RS4;11
Date published: 2020-01-17
Rated 4 out of 5 by from Great for ImmunoHistologyTried the Sample test for Fluorescence ImmunoHistology -Brain perfused with 4% PFA, transcardially -tried embedded AND non-embedded free-floting section (40um-- works better on non-embedded) -"Antigene retrieval" at 80° in 10mM NatriumCitrate -PBS buffer -Concentration 1:100 and 1:200
Date published: 2018-01-04
Rated 5 out of 5 by from Great Western blot data of KCTD12 expressionGreat Western blot data of KCTD12 expression in mouse embryo tissue extract. -SCBT QC
Date published: 2014-12-10
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KCTD12 抗体 (F-6) is rated 4.3 out of 5 by 3.
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