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Katanin p60 AL1 Antibody (A-10) - Immunofluorescence - Image 149295
Katanin p60 AL1 抗体 (A-10): sc-373814. 免疫荧光染色甲醇固定的 HeLa 细胞, 显示细胞骨架定位.
Katanin p60 AL1 抗体 (A-10): sc-373814. 免疫荧光染色甲醇固定的 HeLa 细胞, 显示细胞骨架定位.

Katanin p60 AL1 抗体 (A-10): sc-373814

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说明书
  • Katanin p60 AL1 抗体 A-10 是小鼠单克隆 IgG1 κ，Katanin p60 AL1抗体, 在6篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Katanin p60 AL1的内部区域内的氨基酸72-109之间
  • Katanin p60 AL1 抗体 (A-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Katanin p60 AL1; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗Katanin p60 AL1抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Katanin p60 AL1 (A-10): sc-373814的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Katanin p60 AL1 Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Katanin p60 AL1 Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Katanin抗体，包括Katanin p60 AL1

Katanin p60 AL1 抗体（A-10）是一种 IgG1 κ 小鼠单克隆 Katanin p60 AL1 抗体（也称为 Katanin p60 AL1 抗体），可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类来源的 Katanin p60 AL1 蛋白。Katanin p60 AL1 抗体（A-10）既可以是非偶联的抗 Katanin p60 AL1 抗体形式，也可以是多种偶联形式的抗 Katanin p60 AL1 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。微管是由 α 和 β 亚基组成的聚合物，形成有丝分裂纺锤体，并在间期协助膜性细胞器的组织。Katanin 是一种异源二聚体复合物，以 ATP 依赖的方式切断微管。Katanin 复合物对微管的切断可能促进细胞微管阵列的重组和在成核后从中心体释放微管。Katanin 复合物由 60 kDa 亚基（Katanin p60 A1）和 80 kDa 辅助蛋白（Katanin p80 B1）组成。Katanin p60 A1 负责微管的切断和解聚，而 Katanin p80 B1 将复合物靶向中心体。Katanin p60 A1 和 Katanin p80 B1 属于 AAA ATPase 家族，该家族还包括 Katanin p60 A1 样蛋白、Katanin p60 AL1 和 Katanin p60 AL2。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Katanin p60 AL1 抗体 (A-10) 参考文献:

  1. 卡塔宁在切断神经元微管中的重要作用  |  Ahmad, FJ., et al. 1999. J Cell Biol. 145: 305-15. PMID: 10209026
  2. p80 katanin的两个结构域通过p60 katanin调节微管切断和纺锤极靶向。  |  McNally, KP., et al. 2000. J Cell Sci. 113 (Pt 9): 1623-33. PMID: 10751153
  3. 轴突生长对卡塔宁（一种能切断微管的蛋白质）的水平非常敏感。  |  Karabay, A., et al. 2004. J Neurosci. 24: 5778-88. PMID: 15215300
  4. 磷酸化的 NDEL1（一种与 LIS1 相互作用的蛋白）招募 katanin p60 对有丝分裂细胞分裂和神经元迁移至关重要。  |  Toyo-Oka, K., et al. 2005. Hum Mol Genet. 14: 3113-28. PMID: 16203747
  5. LAPSER1/LZTS2：与抑制卡塔宁介导的微管切断有关的多能肿瘤抑制因子。  |  Sudo, H. and Maru, Y. 2008. Hum Mol Genet. 17: 2524-40. PMID: 18490357
  6. 鉴定 katanin--一种能切断和分解稳定微管的 ATP 酶。  |  McNally, FJ. and Vale, RD. 1993. Cell. 75: 419-29. PMID: 8221885
  7. Katanin 是一种微管切断 ATP 酶，集中在中心体上。  |  McNally, FJ., et al. 1996. J Cell Sci. 109 (Pt 3): 561-7. PMID: 8907702
  8. Katanin是一种微管切割蛋白，它是一种新型AAA ATP酶，利用含WD40的亚基靶向中心体。  |  Hartman, JJ., et al. 1998. Cell. 93: 277-87. PMID: 9568719

订购信息

产品名称产品编号规格价格数量收藏夹

Katanin p60 AL1 抗体 (A-10)

sc-373814
200 µg/ml
RMB2377.00

Katanin p60 AL1 (A-10): m-IgG Fc BP-HRP 套装

sc-529574
200 µg Ab; 10 µg BP
RMB2662.00

Katanin p60 AL1 (A-10): m-IgGκ BP-HRP 套装

sc-522512
200 µg Ab, 40 µg BP
RMB2662.00

Katanin p60 AL1 (A-10): m-IgG1 BP-HRP 套装

sc-543589
200 µg Ab; 20 µg BP
RMB2662.00

Katanin p60 AL1 抗体 (A-10) AC

sc-373814 AC
500 µg/ml, 25% agarose
RMB3129.00

Katanin p60 AL1 抗体 (A-10) HRP

sc-373814 HRP
200 µg/ml
RMB2377.00

Katanin p60 AL1 抗体 (A-10) FITC

sc-373814 FITC
200 µg/ml
RMB2482.00

Katanin p60 AL1 抗体 (A-10) PE

sc-373814 PE
200 µg/ml
RMB2580.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 488

sc-373814 AF488
200 µg/ml
RMB2685.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 546

sc-373814 AF546
200 µg/ml
RMB2685.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 594

sc-373814 AF594
200 µg/ml
RMB2685.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 647

sc-373814 AF647
200 µg/ml
RMB2685.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 680

sc-373814 AF680
200 µg/ml
RMB2685.00

Katanin p60 AL1 抗体 (A-10) Alexa Fluor® 790

sc-373814 AF790
200 µg/ml
RMB2685.00

Katanin p60 AL1 (A-10) 中和勝肽

sc-373814 P
100 µg/0.5 ml
RMB511.00

I am confused about this Ab. It is raised using antigens from the Katanin-like 1 protein, but information on the website including the reference leads to think that it also stains Katanin. Does this antibody effectively stain Katanin(p60)? thanks

Asked by: tommaso C
Thank you for your question. This antibody is raised against a peptide antigen that is specific for Katanin p60 AL1, Katanin p60 subunit A-like 1 (KATNAL1). It is not expected to cross-react with Katanin p60 A1 (KATNA1).
Answered by: Technical Support
Date published: 2019-07-13

What application is the blocking peptide sc-373814 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24

Is it working with katanin p60 obtained from plants? (Zeamays)

Asked by: Sattam
Thank you for your question. We have not tested this Katanin p60 AL1 Antibody (A-10): sc-373814 on any plant samples. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-02-07
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Rated 3 out of 5 by from worked well on western blotThe antibody worked well on extracts from murine primary osteoclasts (dilution 1/200) in western blot (one band at the expected size). However I could not make it work in immunofluorescence (PHEM or methanol fixation).
Date published: 2017-10-09
Rated 5 out of 5 by from Katanin ReviewThe antibody works very well in Hela cells,used at 1:1000
Date published: 2017-07-11
Rated 5 out of 5 by from Beautiful immunofluorescence cytoskeletalBeautiful immunofluorescence cytoskeletal staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-02
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Katanin p60 AL1 抗体 (A-10) is rated 4.5 out of 5 by 4.
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