Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
JNK2 Antibody (A-7) - Western Blotting - Image 377560
JNK2 抗体 (A-7): sc-271133. HL-60 (A), RAW 264. 7 (B), SUP-T1 (C), SJRH30 (D) 和 WEHI-231 (E) 全细胞裂解液中 JNK2 表达的 Western 印迹分析.
JNK2 抗体 (A-7): sc-271133. HL-60 (A), RAW 264. 7 (B), SUP-T1 (C), SJRH30 (D) 和 WEHI-231 (E) 全细胞裂解液中 JNK2 表达的 Western 印迹分析.

JNK2 抗体 (A-7): sc-271133

4.6(9)
寫評論提問

说明书
  • JNK2 抗体 A-7 是小鼠单克隆 IgG1 κ，JNK2抗体, 在14篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的JNK2的C-terminus的氨基酸391-424之间
  • JNK2 抗体 (A-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 JNK2 p54 isoforms; 也和以下物种反应，包括： 和 canine and bovine
  • 抗JNK2抗体（A-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取JNK2 (A-7): sc-271133的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是JNK2 Antibody (A-7) for WB applications. 的首选辅助检测试剂，这些试剂现与JNK2 Antibody (A-7) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外JNK抗体，包括JNK1, JNK2和JNK3

JNK2抗体（A-7）是一种IgG1 κ小鼠单克隆JNK2抗体（也称为MAPK9抗体或SAPK抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的JNK2蛋白。JNK2抗体（A-7）可作为非偶联抗JNK2抗体形式以及多种偶联形式的抗JNK2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Jun N端激酶（JNKs）使c-Jun磷酸化并增强其转录活性。JNKs来源于三个基因，通过不同的mRNA剪接产生10种异构体，包括代表p46异构体的JNK1α1、JNK1β1、JNK2α1、JNK2β1和JNK3α1，以及代表p54异构体的JNK1α2、JNK1β2、JNK2α2、JNK2β2和JNK3β2。JNKs协调细胞对压力的反应，并影响细胞生长和转化的调节。人类JNK1（PRKM8、SAPK1、MAPK8）基因定位于染色体10q11.22，与JNK2具有83%的氨基酸同一性。JNK1对于CD4辅助性T（TH）细胞正常激活和分化为TH1和TH2效应细胞是必需的。辣椒素在ras转化的人乳腺上皮细胞中激活JNK1和p38。氮氧化物（NOx）除了上调c-Fos、c-Jun和其他信号激酶（包括MEKK1和p38）外，还上调JNK1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JNK2 抗体 (A-7) 参考文献:

  1. 抑制 c-Jun N 端激酶 2 的表达能以 p53 依赖性方式抑制人类肿瘤细胞的生长并诱导其凋亡。  |  Potapova, O., et al. 2000. Mol Cell Biol. 20: 1713-22. PMID: 10669748
  2. 效应 T 细胞的功能需要 JNK，而 T 细胞的活化则不需要。  |  Dong, C., et al. 2000. Nature. 405: 91-4. PMID: 10811224
  3. 人单核细胞/巨噬细胞（THP-1）细胞中的 Jun 激酶同工酶有不同的表达和活化形式。  |  Dreskin, SC., et al. 2001. J Immunol. 166: 5646-53. PMID: 11313405
  4. 氮氧化物通过直接和间接激活 MEKK1, JNK 和 p38 信号诱导人 MRC-5 细胞增殖。  |  Chou, FP., et al. 2002. Toxicol Appl Pharmacol. 181: 203-8. PMID: 12079429
  5. 在瑞士 3T3 成纤维细胞中表达的特定功能增益 JNK1 蛋白对基因的不同调控。  |  Han, SY., et al. 2002. J Biol Chem. 277: 47167-74. PMID: 12354774
  6. JNK-1和p38在辣椒素选择性诱导ras转化的人类乳腺上皮细胞凋亡中的作用  |  Kang, HJ., et al. 2003. Int J Cancer. 103: 475-82. PMID: 12478662
  7. JNK2 包含一个特异性决定区，负责有效结合 c-Jun 并使其磷酸化。  |  Kallunki, T., et al. 1994. Genes Dev. 8: 2996-3007. PMID: 8001819
  8. 缺乏 Jnk1 时的 T 细胞分化缺陷。  |  Dong, C., et al. 1998. Science. 282: 2092-5. PMID: 9851932

订购信息

产品名称产品编号规格价格数量收藏夹

JNK2 抗体 (A-7)

sc-271133
200 µg/ml
RMB2377.00

JNK2 (A-7): m-IgG Fc BP-HRP 套装

sc-527210
200 µg Ab; 10 µg BP
RMB2662.00

JNK2 (A-7): m-IgG1 BP-HRP 套装

sc-532583
200 µg Ab; 20 µg BP
RMB2662.00

JNK2 抗体 (A-7) AC

sc-271133 AC
500 µg/ml, 25% agarose
RMB3129.00

JNK2 抗体 (A-7) HRP

sc-271133 HRP
200 µg/ml
RMB2377.00

JNK2 抗体 (A-7) FITC

sc-271133 FITC
200 µg/ml
RMB2482.00

JNK2 抗体 (A-7) PE

sc-271133 PE
200 µg/ml
RMB2580.00

JNK2 抗体 (A-7) Alexa Fluor® 488

sc-271133 AF488
200 µg/ml
RMB2685.00

JNK2 抗体 (A-7) Alexa Fluor® 546

sc-271133 AF546
200 µg/ml
RMB2685.00

JNK2 抗体 (A-7) Alexa Fluor® 594

sc-271133 AF594
200 µg/ml
RMB2685.00

JNK2 抗体 (A-7) Alexa Fluor® 647

sc-271133 AF647
200 µg/ml
RMB2685.00

JNK2 抗体 (A-7) Alexa Fluor® 680

sc-271133 AF680
200 µg/ml
RMB2685.00

JNK2 抗体 (A-7) Alexa Fluor® 790

sc-271133 AF790
200 µg/ml
RMB2685.00

JNK2 (A-7) 中和勝肽

sc-271133 P
100 µg/0.5 ml
RMB511.00

I'd like to consult the immunogen of the antibody (JNK2 antibody (A-7): sc-271133). We want to study a truncated protein, so we want to make sure that the antibody binds to that truncated part.

Asked by: Du Pont
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hi there, I have used this antibody in parallel with a JNK1 antibody we've already had from cell signaling to compare the relative levels. The size of the dominant isoform of JNK2 (p54) however is larger than I expected (definitely >60kDa)

Asked by: YG986
Thank you for your question. This is consistent with the molecular weight that we have observed using this mouse monoclonal antibody, JNK2 (A-7): sc-271133, for endogenous detection in a variety of whole cell lysates. This data is currently available on our website as a reference. If you would like help in troubleshooting the antibody, please contact our Technical Service Department. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-10-25

What application is the blocking peptide sc-271133 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_5, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_271133, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Not very goodI had tested it in C42B and VCaP cell lines. It gets a dark background.
Date published: 2017-10-11
Rated 4 out of 5 by from WB ResultsI applied for a sample two week ago.My sample is the mouse liver. Incubated the antibody for overnight at 4℃, 1:1000 dilution, and  m-IgGκ BP-HRP  incubated for 1 hour at RT , 1:1000 dilution.The results are shown in the figure.
Date published: 2017-09-16
Rated 5 out of 5 by from good antibodyI used this antibody for wb of HUVEC, with a dilution of 1:500 ,the band is clear!
Date published: 2017-08-14
Rated 5 out of 5 by from Nice AntibodyI received a free sample last month, and did a test on THP-1 cells and DO11.10 cells. And it works well.
Date published: 2017-05-08
Rated 4 out of 5 by from good resultI used tiis antibody in wb ,and got a good band. The result is satisfactory.
Date published: 2017-02-17
Rated 5 out of 5 by from Good antibody resultsWe used this antibody for detection in HeLa cells. We got a positive band when looking at the western blot
Date published: 2017-01-12
Rated 4 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-11-02
Rated 5 out of 5 by from Published with this antibody for western blotPublished with this antibody for western blot. -SCBT Publication Review
Date published: 2015-01-22
  • y_2025, m_9, d_5, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_271133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
JNK2抗体 (A-7) is rated 4.6 out of 5 by 9.
  • y_2025, m_9, d_5, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_271133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。