Date published: 2025-9-6
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JNK1 Antibody (D-6) - Western Blotting - Image 373050
JNK1 抗体 (D-6): sc-137018. NIH/3T3 (A), Raji (B), BJAB (C), HEL 92. 1. 7 (D) 和 PC-12 (E) 全细胞裂解液中 JNK1 表达的 Western 印迹分析.
JNK1 抗体 (D-6): sc-137018. NIH/3T3 (A), Raji (B), BJAB (C), HEL 92. 1. 7 (D) 和 PC-12 (E) 全细胞裂解液中 JNK1 表达的 Western 印迹分析.

JNK1 抗体 (D-6): sc-137018

4.9(8)
寫評論提問

说明书
  • JNK1抗体(D-6)是小鼠单克隆IgG2b κ, 在24篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-384的抗原，代表全长JNK1 p461 p46，源自human
  • 抗-JNK1 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 JNK1 p46 and p54 isoforms
  • 抗JNK1抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取JNK1 (D-6): sc-137018的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 JNK1 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与JNK1 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加JNK抗体，包括JNK1, JNK2和JNK3

JNK1抗体（D-6）是一种IgG2b κ小鼠单克隆JNK1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的JNK1 p46和p54异构体。JNK1抗体（D-6）可作为非偶联抗JNK1抗体形式使用，也可作为多种偶联形式的抗JNK1抗体使用，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Jun N-末端激酶（JNKs）通过磷酸化作用增强c-Jun的转录活性。JNKs由三个基因通过选择性mRNA剪接产生10种异构体，包括代表p46异构体的JNK1a1、JNK1b1、JNK2a1、JNK2b1和JNK3a1，以及代表p54异构体的JNK1a2、JNK1b2、JNK2a2、JNK2b2和JNK3b2。JNKs协调细胞对压力的反应并影响细胞生长和转化的调节。人类JNK1（PRKM8、SAPK1、MAPK8）基因位于染色体10q11.22，与JNK2具有83%的氨基酸同源性。JNK1对于CD4辅助T（TH）细胞正常激活和分化为TH1和TH2效应细胞是必需的。辣椒素在ras转化的人乳腺上皮细胞中激活JNK1和p38。氮氧化物（NOx）除了上调c-Fos、c-Jun和其他信号激酶（包括MEKK1和p38）外，还上调了JNK1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

JNK1 抗体 (D-6)

sc-137018
200 µg/ml
RMB2377.00

JNK1 (D-6): m-IgG Fc BP-HRP 套装

sc-525753
200 µg Ab; 10 µg BP
RMB2662.00

JNK1 抗体 (D-6) AC

sc-137018 AC
500 µg/ml, 25% agarose
RMB3129.00

JNK1 抗体 (D-6) HRP

sc-137018 HRP
200 µg/ml
RMB2377.00

JNK1 抗体 (D-6) FITC

sc-137018 FITC
200 µg/ml
RMB2482.00

JNK1 抗体 (D-6) PE

sc-137018 PE
200 µg/ml
RMB2580.00

JNK1 抗体 (D-6) Alexa Fluor® 488

sc-137018 AF488
200 µg/ml
RMB2685.00

JNK1 抗体 (D-6) Alexa Fluor® 546

sc-137018 AF546
200 µg/ml
RMB2685.00

JNK1 抗体 (D-6) Alexa Fluor® 594

sc-137018 AF594
200 µg/ml
RMB2685.00

JNK1 抗体 (D-6) Alexa Fluor® 647

sc-137018 AF647
200 µg/ml
RMB2685.00

JNK1 抗体 (D-6) Alexa Fluor® 680

sc-137018 AF680
200 µg/ml
RMB2685.00

JNK1 抗体 (D-6) Alexa Fluor® 790

sc-137018 AF790
200 µg/ml
RMB2685.00

What is the species cross-reactivity of this antibody?

Asked by: Zocco
Thank you for your question. This antibody sc-137018 is recommended for detection of JNK1 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you are interested in receiving a sample of this antibody to test in a different species, please contact our Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31

I am using JNK1 (D-6): sc-137018 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 很好用，条带清晰解决了我的燃眉之急，之前买的抗体不够用，又不好意思向老板申请再重新买，于是就和师妹一起申请了试用装，感谢感谢
Date published: 2021-05-31
Rated 4 out of 5 by from 没有孵出图片那么明显的条带和图片示例的两条带略微有些差异，不知道是否是因为我切割了膜的关系，不过还是可以较为明显的看出来目的条带
Date published: 2017-10-20
Rated 5 out of 5 by from Very goodI had tested it in C42B and VCaP cell lines. It works very good.
Date published: 2017-10-11
Rated 5 out of 5 by from greatI am so happy with the sensitivity of the antibody.
Date published: 2017-09-28
Rated 5 out of 5 by from Great for IHC(P)The JNK1 Antibody (D-6): sc-137018 works very well for immunohistochemistry with formalin fixed, paraffin-embedded tissue sections, showing staining pattern localized to the cytoplasm and nucleus. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-30
Rated 5 out of 5 by from Robust nuclear and cytoplasmic staining inRobust nuclear and cytoplasmic staining in paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Great WB data with publication in mouse ratGreat WB data with publication in mouse rat and human samples: Rat HCC and McA-RH7777, mouse MEC and Svec4-10EE2, and human Hek 293. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Positive WB data in RAW 264Positive WB data in RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-02-16
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JNK1 抗体 (D-6) is rated 4.9 out of 5 by 8.
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