Date published: 2025-9-6
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JNK Antibody (D-2) - Western Blotting - Image 396283
JNK 抗体 (D-2) Alexa Fluor® 680: sc-7345 AF680. K-562 (A), HeLa (B) 和 HEL 92. 1. 7 (C) 全细胞裂解液中 JNK 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
JNK 抗体 (D-2) Alexa Fluor® 680: sc-7345 AF680. K-562 (A), HeLa (B) 和 HEL 92. 1. 7 (C) 全细胞裂解液中 JNK 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

JNK 抗体 (D-2): sc-7345

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说明书
  • JNK 抗体 D-2 是小鼠单克隆 IgG1 κ，JNK抗体, 在705篇文献中引用，规格为200 µg/ml
  • 免疫1-424氨基酸，对应于human来源JNK2 p54全长
  • JNK 抗体 (D-2) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 all JNK1, JNK2 and JNK3 p46 and p54 isoforms
  • 抗JNK抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取JNK (D-2): sc-7345的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 JNK 抗体 (D-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与JNK 抗体 (D-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 JNK 抗体包括 JNK1, JNK2 和 JNK3

JNK抗体（D-2）是一种小鼠单克隆IgG1 kappa轻链抗体，针对全长人类JNK2（氨基酸1-424）产生，后者是一种重要的应激激活蛋白激酶。JNK小鼠单克隆抗体（D-2）可识别JNK免疫原序列中的表位，并表现出广泛的物种交叉反应性，可通过蛋白质印迹（WB）、免疫沉淀（IP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的JNK蛋白。抗JNK单克隆抗体（D-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物，可满足各种实验需求。JNK蛋白（也称为MAPK8/9/10或SAPK/JNK）作为丝氨酸/苏氨酸激酶，通过磷酸化并激活包括c-Jun、ATF2和Elk-1在内的转录因子，对压力刺激、细胞因子和生长因子做出反应。三种不同的基因（JNK1、JNK2和JNK3）通过可变mRNA剪接产生十种不同的同工型，包括p46和p54变体，每种变体都有助于调节细胞过程，如增殖、分化、凋亡和应激反应。JNK参与复杂的蛋白质网络，与JIP（JNK相互作用蛋白）、参与细胞凋亡调控的Bcl-2家族成员等支架蛋白以及MEKK1和p38 MAPK等其他信号激酶形成复合物。这些相互作用形成了复杂的信号级联，影响细胞生长和转化。当JNK信号传导失调时，由此产生的破坏会导致包括癌症、神经退行性疾病、糖尿病和心血管疾病在内的各种疾病。JNK (D-2) 抗体与所有 JNK1、JNK2 和 JNK3 p46 和 p54 异构体广泛反应，是研究这些复杂信号网络及其在正常细胞功能和疾病状态中作用的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JNK 抗体 (D-2) 参考文献:

  1. JNK 对代谢综合征肝脏表现的调控。  |  Czaja, MJ. 2010. Trends Endocrinol Metab. 21: 707-13. PMID: 20888782
  2. JNK 信号： 基于复杂蛋白-蛋白伙伴关系的调控和功能。  |  Zeke, A., et al. 2016. Microbiol Mol Biol Rev. 80: 793-835. PMID: 27466283
  3. 癌细胞存活中的 JNK 信号传导。  |  Wu, Q., et al. 2019. Med Res Rev. 39: 2082-2104. PMID: 30912203
  4. 脊髓神经元发育和死亡中的 JNK 信号通路。  |  Schellino, R., et al. 2019. Cells. 8: PMID: 31817379
  5. JNK 信号的选择性抑制剂：一种潜在的癌症靶向疗法  |  Wu, Q., et al. 2020. J Enzyme Inhib Med Chem. 35: 574-583. PMID: 31994958
  6. 炎症性皮肤病和癌症中的 JNK 信号通路。  |  Hammouda, MB., et al. 2020. Cells. 9: PMID: 32252279
  7. Slik 通过阻止 JNK 介导的细胞凋亡维持组织稳态  |  Li, C., et al. 2023. Cell Div. 18: 16. PMID: 37794497
  8. JNK通过星间转运复合体和肌动蛋白网络调控纤毛的生成  |  Chatzifrangkeskou, M., et al. 2023. J Cell Biol. 222: PMID: 37851005
  9. JNK信号通路失调在人类疾病发病机制中的作用及其潜在治疗策略》： 全面综述》。  |  Yan, H., et al. 2024. Biomolecules. 14: PMID: 38397480

订购信息

产品名称产品编号规格价格数量收藏夹

JNK 抗体 (D-2)

sc-7345
200 µg/ml
$316.00

JNK (D-2): m-IgG Fc BP-HRP 套装

sc-528179
200 µg Ab; 10 µg BP
$354.00

JNK (D-2): m-IgGκ BP-HRP 套装

sc-520496
200 µg Ab, 40 µg BP
$354.00

JNK (D-2): m-IgG1 BP-HRP 套装

sc-542804
200 µg Ab; 20 µg BP
$354.00

JNK 抗体 (D-2) AC

sc-7345 AC
500 µg/ml, 25% agarose
$416.00

JNK 抗体 (D-2) HRP

sc-7345 HRP
200 µg/ml
$316.00

JNK 抗体 (D-2) FITC

sc-7345 FITC
200 µg/ml
$330.00

JNK 抗体 (D-2) PE

sc-7345 PE
200 µg/ml
$343.00

JNK 抗体 (D-2) Alexa Fluor® 488

sc-7345 AF488
200 µg/ml
$357.00

JNK 抗体 (D-2) Alexa Fluor® 546

sc-7345 AF546
200 µg/ml
$357.00

JNK 抗体 (D-2) Alexa Fluor® 594

sc-7345 AF594
200 µg/ml
$357.00

JNK 抗体 (D-2) Alexa Fluor® 647

sc-7345 AF647
200 µg/ml
$357.00

JNK 抗体 (D-2) Alexa Fluor® 680

sc-7345 AF680
200 µg/ml
$357.00

JNK 抗体 (D-2) Alexa Fluor® 790

sc-7345 AF790
200 µg/ml
$357.00

JNK 抗体 (D-2) B

sc-7345 B
200 µg/ml
$320.00

Hi, Is this okay to use in confocal microscopy? Thanks

Asked by: Roshan
Thank you for your question. JNK Antibody (D-2): sc-7345 is recommended for immunofluorescence and immunohistochemistry (among other applications) and can be used in confocal microscopy of cell cultures and tissues. We also offer AlexaFluor- and HRP-conjugated versions of this antibody which are ideally suited for such applications. Feel free to get in touch with our technical service for more information.
Answered by: Tech Support Europe
Date published: 2019-05-09

Hi, Is this antibody reactive with Oncorhynchus mykiss?

Asked by: Yding
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support 10
Date published: 2017-11-28

Hello. Is there a safety data sheet that goes with JNK Antibody (D-2): sc-7345 cat# sc-7345? If so, please forward it to me. Thanks.

Asked by: Vanessa3321
Thank you for your question. The antibodies provided by Santa Cruz Biotechnology are non-hazardous reagents, for which no SDS is required or provided. This includes this mouse monoclonal, JNK (D-2): sc-7345. If you require any documentation stating this, please contact our Technical Service Department. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-19

What secondary reagent should I use for detection of JNK Antibody (D-2) in immunohistochemical applications using paraffin-embedded sections?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-FITC: sc-516140, for IHC(P). A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-16

Is it possible for JNK Antibody (D-2) to work with guinea pig samples?

Asked by: Trav11
To check additional species reactivity, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-16
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Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 5 out of 5 by from Great for WBIt is a good antibody for western blot. I'd recommend it.
Date published: 2024-03-19
Rated 5 out of 5 by from Total JNK antibodyrobust signal in correct size using western blot. highly recommended.
Date published: 2023-04-12
Rated 5 out of 5 by from Great antibody-JNKThis antibody worked great for me on western blot.
Date published: 2021-12-03
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from Very good for WBThis antibody is very good for WB using human K562 whole cell lysate at 1:1000 dilution.
Date published: 2021-08-05
Rated 5 out of 5 by from Remarkable features!This antibody works great in western blot analysis in Rat Brain Astrocytes.
Date published: 2021-07-20
Rated 4 out of 5 by from western blotThis primary antibody is useful in western blotting
Date published: 2021-02-08
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JNK 抗体 (D-2) is rated 4.8 out of 5 by 82.
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