Date published: 2025-9-7
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JAM-A Antibody (J10.4) - Western Blotting - Image 13981
JAM-A 抗体 (J10. 4): sc-53623. 还原 (A, B, C) 和非还原 (D, E, F) 条件下, 人血小板提取物 (A, D) 和人 PBL (B, E) 及 T84 (C, F) 全细胞裂解液中 JAM-A 表达的 Western 印迹分析.
JAM-A 抗体 (J10. 4): sc-53623. 还原 (A, B, C) 和非还原 (D, E, F) 条件下, 人血小板提取物 (A, D) 和人 PBL (B, E) 及 T84 (C, F) 全细胞裂解液中 JAM-A 表达的 Western 印迹分析.

JAM-A 抗体 (J10.4): sc-53623

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说明书
  • JAM-A 抗体 J10.4 是小鼠单克隆 IgG1 κ，JAM-A抗体, 在37篇文献中引用，规格为200 µg/ml
  • 针对human的重组JAM融合蛋白的抗体
  • JAM-A 抗体 (J10.4) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 JAM-A
  • 抗JAM-A抗体（J10.4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取JAM-A (J10.4): sc-53623的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是JAM-A Antibody (J10.4) for WB applications. 的首选辅助检测试剂，这些试剂现与JAM-A Antibody (J10.4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加JAM抗体，包括JAM-A, JAM-B, JAM-C和JAM4

JAM-A抗体（J10.4）是一种IgG1 κ小鼠单克隆JAM-A抗体（也称为JCAM抗体、血小板粘附分子1（PAM-1）抗体或连接粘附分子A抗体），可通过WB、IP、IF和FCM检测小鼠、大鼠和人源的JAM-A蛋白。JAM-A抗体（J10.4）既可以作为非偶联抗JAM-A抗体形式提供，也可以作为多种偶联形式的抗JAM-A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。连接粘附分子（JAM）是表达于上皮细胞和内皮细胞的紧密连接处的免疫球蛋白超家族的成员。它与白细胞的跨内皮迁移有关。JAM在循环单核细胞、中性粒细胞、淋巴细胞亚群和血小板上持续表达。JAM家族由JAM-A、JAM-B和JAM-C组成，也可分别命名为JAM-1、JAM-2和JAM-3。JAM-A通过与F-Actin的定位在细胞-细胞接触处和膜皱褶处。它通过同型相互作用参与细胞间粘附，并在组织紧密连接和调节白细胞渗出方面发挥作用。JAM-B与离散的PBL亚群相互作用，表明它可能在淋巴细胞迁移中发挥作用。JAM-B和JAM-C蛋白是结合伙伴；JAM-C可能是功能性JAM-B受体。具体来说，JAM-B通过与JAM-C的异型相互作用粘附到T细胞上。JAM-B/JAM-C相互作用可能在T细胞、NK细胞和树突状细胞炎症中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JAM-A 抗体 (J10.4) 参考文献:

  1. 前沿：TNF-α 和 IFN-gamma 的联合治疗会导致人内皮细胞中连接粘附分子的重新分布。  |  Ozaki, H., et al. 1999. J Immunol. 163: 553-7. PMID: 10395639
  2. 交界粘附分子与含 PDZ 结构域的蛋白 AF-6 和 ZO-1 相互作用。  |  Ebnet, K., et al. 2000. J Biol Chem. 275: 27979-88. PMID: 10856295
  3. 交界粘附分子的同嗜性相互作用。  |  Bazzoni, G., et al. 2000. J Biol Chem. 275: 30970-6. PMID: 10913139
  4. 血小板活化时，交界粘附分子（JAM）会被蛋白激酶 C 磷酸化。  |  Ozaki, H., et al. 2000. Biochem Biophys Res Commun. 276: 873-8. PMID: 11027562
  5. 内皮连接的分子组织及其在血管形态形成和通透性中的功能作用。  |  Dejana, E., et al. 2000. Int J Dev Biol. 44: 743-8. PMID: 11061439
  6. 克隆人类交界黏合剂分子 3（JAM3）并将其鉴定为 JAM2 的反受体。  |  Arrate, MP., et al. 2001. J Biol Chem. 276: 45826-32. PMID: 11590146
  7. 血管内皮-功能粘合剂分子（VE-JAM）/JAM 2 通过 JAM 3 与 T, NK 和树突状细胞相互作用。  |  Liang, TW., et al. 2002. J Immunol. 168: 1618-26. PMID: 11823489
  8. JAM2 与 alpha4beta1 相互作用。JAM3 起促进作用。  |  Cunningham, SA., et al. 2002. J Biol Chem. 277: 27589-92. PMID: 12070135
  9. 交界粘附分子-2（JAM-2）可促进淋巴细胞跨内皮迁移。  |  Johnson-Léger, CA., et al. 2002. Blood. 100: 2479-86. PMID: 12239159
  10. 交界粘附分子是免疫球蛋白超家族的一个新成员，它分布在细胞间的交界处并调节单核细胞的迁移。  |  Martìn-Padura, I., et al. 1998. J Cell Biol. 142: 117-27. PMID: 9660867

订购信息

产品名称产品编号规格价格数量收藏夹

JAM-A 抗体 (J10.4)

sc-53623
200 µg/ml
RMB2377.00

JAM-A (J10.4): m-IgG Fc BP-HRP 套装

sc-526838
200 µg Ab; 10 µg BP
RMB2662.00

JAM-A (J10.4): m-IgG1 BP-HRP 套装

sc-532211
200 µg Ab; 20 µg BP
RMB2662.00

JAM-A 抗体 (J10.4) AC

sc-53623 AC
500 µg/ml, 25% agarose
RMB3129.00

JAM-A 抗体 (J10.4) HRP

sc-53623 HRP
200 µg/ml
RMB2377.00

JAM-A 抗体 (J10.4) FITC

sc-53623 FITC
200 µg/ml
RMB2482.00

JAM-A 抗体 (J10.4) PE

sc-53623 PE
200 µg/ml
RMB2580.00

JAM-A 抗体 (J10.4) Alexa Fluor® 488

sc-53623 AF488
200 µg/ml
RMB2685.00

JAM-A 抗体 (J10.4) Alexa Fluor® 546

sc-53623 AF546
200 µg/ml
RMB2685.00

JAM-A 抗体 (J10.4) Alexa Fluor® 594

sc-53623 AF594
200 µg/ml
RMB2685.00

JAM-A 抗体 (J10.4) Alexa Fluor® 647

sc-53623 AF647
200 µg/ml
RMB2685.00

JAM-A 抗体 (J10.4) Alexa Fluor® 680

sc-53623 AF680
200 µg/ml
RMB2685.00

JAM-A 抗体 (J10.4) Alexa Fluor® 790

sc-53623 AF790
200 µg/ml
RMB2685.00

Using this antibody (sc-53623), I get two bands in WB in tissues, but only one band in cell lysates. I want to know why

Asked by: Anonymous
Thank you for your question. Please call or email technical service. (800)-457-3801 or email [email protected]
Answered by: BlakeJ
Date published: 2023-06-27

Can this antibody be used for staining mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated antibody. JAM-A Antibody (J10.4): sc-53623 is available conjugated to HRP (sc-53623 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-53623 PE), fluorescein (sc-53623 FITC), Alexa Fluor® 488 (sc-53623 AF488) or Alexa Fluor® 647 (sc-53623 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Service 11
Date published: 2017-01-13

What is the recommended secondary for this mouse primary antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13
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Rated 5 out of 5 by from Good result in IF and WBJAK1 in CRFK cells, dilution 1:50. Good result, no background
Date published: 2025-06-24
Rated 5 out of 5 by from 使用感不错我之前买了这个抗体，实验很顺利，感谢贵司人员的辛勤工作，帮助了我的科研，以后我会多多关注这个品牌，希望多出好抗体
Date published: 2022-10-31
Rated 5 out of 5 by from good quality productThis antibody is good quality give us specific bands,the stable quality can keep more than 1year for WB application.Always specific bands at 36kda.
Date published: 2018-01-27
Rated 4 out of 5 by from Works good on WBThe antibody worked well on western blot on mouse tissue sample. It showed a clear band around 36kD. I also tried on IF but the result was not conclusive.
Date published: 2017-10-25
Rated 5 out of 5 by from specific/sharp bands detectedThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from super IF marker!It works very well for IF applications: IHC and ICC of human tissues
Date published: 2017-06-29
Rated 4 out of 5 by from Human gingival 3D tissueWe got this antibody as a free sample. It works (1/50-1/400) in both frozen and paraffin embedded sections with some background.
Date published: 2017-04-25
Rated 5 out of 5 by from Outstanding productClear, strong western data with little or no background; also great for IF staining applications
Date published: 2017-01-20
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JAM-A 抗体 (J10.4) is rated 4.8 out of 5 by 16.
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