Soluble in ethanol, methanol, DMF or DMSO. Limited water solubility.
TEST CONDITIONS
HPLC Analysis and Mass Spectrum: Chromatogram (205 and 254 nm) on generated using C18 reverse phase column(Alltima, Rocket format, Grace Discovery) under gradient conditions (10 to 100% ACN, 0.01% TFA, 10 min). The product elutes as a single peak at 6.75 min (no cyclosporin A observed) and is detectable only at 200 nm. MS positive ion mode gives M+1 = m/e1202.3 with Na adduct. Purity for batch >98%.