Date published: 2025-9-8
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Islet-1 Antibody (B-1) - Western Blotting - Image 387998
Islet-1 抗体 (B-1) HRP: sc-390793 HRP. 在 HEL 92. 1. 7 核提取物 (A) 和 K-562 (B), MIA PaCa-2 (C), RAW 264. 7 (D), HeLa (E) 和 PC-12 (F) 全细胞裂解液中进行 Islet-1 表达的 Western 印迹分析.
Islet-1 抗体 (B-1) HRP: sc-390793 HRP. 在 HEL 92. 1. 7 核提取物 (A) 和 K-562 (B), MIA PaCa-2 (C), RAW 264. 7 (D), HeLa (E) 和 PC-12 (F) 全细胞裂解液中进行 Islet-1 表达的 Western 印迹分析.

Islet-1 抗体 (B-1): sc-390793

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寫評論提問

说明书
  • Islet-1 抗体 B-1 是小鼠单克隆 IgG1 κ，Islet-1抗体, 在8篇文献中引用，规格为200 µg/ml
  • 与human的244-282氨基酸残基的Islet-1
  • Islet-1 抗体 (B-1) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的Islet-1; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 可用于ChIP的TransCruz试剂(sc-390793 X, 200 µg/0.1 ml)
  • 抗Islet-1抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Islet-1 (B-1): sc-390793的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Islet-1 Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Islet-1 Antibody (B-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Islet抗体，包括Islet-2

Islet-1抗体（B-1）是一种IgG1 κ小鼠单克隆Islet-1抗体（也称为ISL LIM Homeobox 1抗体、胰岛素基因增强子蛋白ISL-1抗体、Islet-1抗体、ISLET1抗体、Isl-1转录因子LIM/同源结构域（Islet-1）或ISL1转录因子LIM/同源结构域抗体），通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人的Islet-1蛋白。Islet-1抗体（B-1）既可以作为非偶联的抗Islet-1抗体形式提供，也可以作为多种偶联形式的抗Islet-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Islet-1（ISL1转录因子，LIM/同源结构域）和Islet-2（ISL2转录因子，LIM/同源结构域）含有氨基末端LIM结构域和羧基末端同源结构域，二者均影响发育过程。Islet-1影响胰岛朗格汉斯氏岛的胚胎发育和神经管运动神经元的分化。在小鼠牙齿发育过程中，Islet-1作为口腔上皮切牙（远端）区域Bmp4表达的激活剂，介导齿列的形成。Islet-1的表达定义了心脏祖细胞群，是正常心脏发育和不对称所必需的。新生成的运动神经元中的Islet-2活性允许发育中的脊髓内脏和躯体运动神经元亚型的多样化。小鼠Islet-2通过以Zic2和EphB1依赖的方式抑制视网膜神经节细胞（RGC）腹侧-时间新月体（VTC）特有的同侧寻路程序，来指定RGC的侧性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Islet-1 抗体 (B-1) 参考文献:

  1. Islet1 在小鼠牙齿形态中的作用  |  Mitsiadis, TA., et al. 2003. Development. 130: 4451-60. PMID: 12900460
  2. Isl1确定了一种心脏祖细胞群，这种祖细胞在分化前会增殖，并为心脏提供大部分细胞。  |  Cai, CL., et al. 2003. Dev Cell. 5: 877-89. PMID: 14667410
  3. Islet-2参与了胚胎斑马鱼感觉神经元轴突分支和脱袢的Slit信号传导。  |  Yeo, SY., et al. 2004. Mech Dev. 121: 315-24. PMID: 15110042
  4. 双目视力的大小受胰岛素 2 抑制遗传程序的控制，该程序规定了视网膜轴突寻路的侧向性。  |  Pak, W., et al. 2004. Cell. 119: 567-78. PMID: 15537545
  5. 内分泌胰腺的成熟, 增殖和存活都需要胰岛-1。  |  Du, A., et al. 2009. Diabetes. 58: 2059-69. PMID: 19502415
  6. 出生前小鼠胸椎运动神经元中 Islet-1 的表达  |  Kobayashi, M., et al. 2011. Int J Dev Neurosci. 29: 749-56. PMID: 21651972
  7. SSBP3 与 Islet-1 和 Ldb1 相互作用，影响胰腺β-细胞的目的基因。  |  Galloway, JR., et al. 2015. Mol Endocrinol. 29: 1774-86. PMID: 26495868
  8. 通过 Islet-1/2 表达检测小鼠胚胎中促性腺激素释放激素-1 神经元的异质性起源  |  Shan, Y., et al. 2020. Front Cell Dev Biol. 8: 35. PMID: 32083082
  9. 太空飞行和微重力对人类胰岛-1+心血管祖细胞转录组的影响  |  Camberos, V., et al. 2021. Int J Mol Sci. 22: PMID: 33808224
  10. LIM 域同源染色体基因 isl-1：人类, 仓鼠和大鼠互补脱氧核糖核酸序列的保存以及在非神经内分泌系细胞类型中的表达。  |  Wang, M. and Drucker, DJ. 1994. Endocrinology. 134: 1416-22. PMID: 7907017
  11. 人类 LIM/homeodomain 基因 islet-1 的分离及简单序列重复多态性的鉴定 [更正]。  |  Tanizawa, Y., et al. 1994. Diabetes. 43: 935-41. PMID: 7912209
  12. 在运动神经元生成过程中对 LIM 同源异形基因 Isl1 的需要揭示了中间神经元分化过程中依赖于运动神经元的一个步骤。  |  Pfaff, SL., et al. 1996. Cell. 84: 309-20. PMID: 8565076

订购信息

产品名称产品编号规格价格数量收藏夹

Islet-1 抗体 (B-1)

sc-390793
200 µg/ml
RMB2377.00

Islet-1 (B-1): m-IgG Fc BP-HRP 套装

sc-530265
200 µg Ab; 10 µg BP
RMB2662.00

Islet-1 (B-1): m-IgGκ BP-HRP 套装

sc-523686
200 µg Ab, 40 µg BP
RMB2662.00

Islet-1 (B-1): m-IgG1 BP-HRP 套装

sc-543964
200 µg Ab; 20 µg BP
RMB2662.00

Islet-1 抗体 (B-1) X

sc-390793 X
200 µg/0.1 ml
RMB2377.00

Islet-1 抗体 (B-1) AC

sc-390793 AC
500 µg/ml, 25% agarose
RMB3129.00

Islet-1 抗体 (B-1) HRP

sc-390793 HRP
200 µg/ml
RMB2377.00

Islet-1 抗体 (B-1) FITC

sc-390793 FITC
200 µg/ml
RMB2482.00

Islet-1 抗体 (B-1) PE

sc-390793 PE
200 µg/ml
RMB2580.00

Islet-1 抗体 (B-1) Alexa Fluor® 488

sc-390793 AF488
200 µg/ml
RMB2685.00

Islet-1 抗体 (B-1) Alexa Fluor® 546

sc-390793 AF546
200 µg/ml
RMB2685.00

Islet-1 抗体 (B-1) Alexa Fluor® 594

sc-390793 AF594
200 µg/ml
RMB2685.00

Islet-1 抗体 (B-1) Alexa Fluor® 647

sc-390793 AF647
200 µg/ml
RMB2685.00

Islet-1 抗体 (B-1) Alexa Fluor® 680

sc-390793 AF680
200 µg/ml
RMB2685.00

Islet-1 抗体 (B-1) Alexa Fluor® 790

sc-390793 AF790
200 µg/ml
RMB2685.00

Islet-1 (B-1) 中和勝肽

sc-390793 P
100 µg/0.5 ml
RMB511.00

Hello, Is sc-390793 appropriate for ICC, IHC application? If so, would this work well with neural cells harvested from mouse?

Asked by: George123
Thank you for your question. Islet-1 Antibody (B-1): sc-390793 is recommended for IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections or for IHC(P). This antibody is recommended for detection of mouse Islet-1, however, we have not specifically tested the antibody on mouse neural cells. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test a sample. Please call us toll-free at (800) 457-3801 option 2, email [email protected], or use our live chat.
Answered by: Technical Support
Date published: 2017-02-27

What application is the blocking peptide sc-390793 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from GoodI used this antibody in IF on human ipsc-derived motoneurons and it provides a specific signal labeling cell nuclei.
Date published: 2022-06-15
Rated 4 out of 5 by from Good antibodyI used it in IF on human cells at 1:100 obtaining a strong and specific signal.
Date published: 2022-06-06
Rated 5 out of 5 by from excellentthe antibody worked perfectly on rat tissue samples in western blot
Date published: 2017-12-13
Rated 5 out of 5 by from GreatI got a sample a month back and it works great for us
Date published: 2017-10-12
Rated 5 out of 5 by from Very good labeling of numerous cell nucleiVery good labeling of numerous cell nuclei in the ventricular regions of the intact brain
Date published: 2017-02-13
Rated 5 out of 5 by from Excellent antibody for WBThe Islet-1 Antibody (B-1): sc-390793 provides great Western blot data, showing clear band with minimal background.
Date published: 2017-01-17
Rated 3 out of 5 by from Normal resultWe test this antibody in human fibroblast sample and in cybrid lines for WB, we can observe different bands in the detection
Date published: 2017-01-10
Rated 1 out of 5 by from does not work on macaque tissueantibody did not detect Islet-1 in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON
Date published: 2016-12-20
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Islet-1 抗体 (B-1) is rated 4.1 out of 5 by 9.
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