Date published: 2025-9-7
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ISG15 Antibody (F-9) - Immunohistochemistry - Image 382983
ISG15 抗体 (F-9): sc-166755. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色显示肝细胞的胞浆染色.
ISG15 抗体 (F-9): sc-166755. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色显示肝细胞的胞浆染色.

ISG15 抗体 (F-9): sc-166755

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说明书
  • ISG15 抗体 F-9 是小鼠单克隆 IgG1 κ，ISG15抗体, 在110篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-150的抗原，在ISG15的N-terminus处映射，human来源
  • ISG15 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ISG15
  • 抗ISG15抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ISG15 (F-9): sc-166755的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ISG15 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ISG15 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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说明书与实验方案
研究信息

関連項目

ISG抗体

ISG15抗体（F-9）是一种IgG1 κ小鼠单克隆ISG15抗体（也称为G1P2抗体、UCRP抗体或干扰素诱导的15 kDa蛋白抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的ISG15蛋白。ISG15抗体（F-9）既可作为非偶联抗ISG15抗体形式提供，也可作为多种偶联形式的抗ISG15抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。干扰素诱导的15 kDa蛋白（ISG15）通过不同于泛素的酶途径与细胞内靶蛋白（如JAK1或MAPK3/ERK1）结合，从而发挥泛素的作用。ISG15对中性粒细胞具有特异的趋化活性，并激活它们以诱导嗜酸性粒细胞趋化因子的释放。ISG15还通过诱导单核细胞和巨噬细胞分泌IFN-γ，参与旁分泌、自分泌和内分泌机制，如在细胞间信号传导中。ISG15是一种主要在肌肉、上皮、神经元和淋巴样细胞中表达的细胞质蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ISG15 抗体 (F-9) 参考文献:

  1. ISG15在抗病毒免疫及其他方面的作用。  |  Perng, YC. and Lenschow, DJ. 2018. Nat Rev Microbiol. 16: 423-439. PMID: 29769653
  2. ISG15，一种具有重大意义的小分子：调控线粒体稳态。  |  Albert, M., et al. 2018. Viruses. 10: PMID: 30428561
  3. ISG15促进ERK1的ISG化，激活CD8+ T细胞，抑制卵巢癌进展。  |  Yeung, TL., et al. 2018. Cancers (Basel). 10: PMID: 30469497
  4. SARS-CoV-2木瓜蛋白酶可拮抗ISG15对传感器MDA5的依赖性激活，从而逃避宿主的先天性免疫。  |  Liu, G., et al. 2021. Nat Microbiol. 6: 467-478. PMID: 33727702
  5. ISG15与宫颈癌发展有关。  |  Tao, P., et al. 2022. Oncol Lett. 24: 380. PMID: 36238852
  6. ISG15：先天免疫信号、DNA复制和基因组稳定性之间的联系。  |  Wardlaw, CP. and Petrini, JHJ. 2023. Bioessays. 45: e2300042. PMID: 37147792
  7. ISG15和ISG化调节癌干细胞样特性，促进甲状腺癌肿瘤生长。  |  Xu, T., et al. 2023. J Exp Clin Cancer Res. 42: 182. PMID: 37501099
  8. ISG15修饰调控DNA感应中的STING活性。  |  Lin, C., et al. 2023. Cell Rep. 42: 113277. PMID: 37864791
  9. ISG15介导细胞外囊泡促进卵巢癌进展和转移的功能。  |  Dorayappan, KDP., et al. 2024. J Extracell Biol. 3: e92. PMID: 38939897

订购信息

产品名称产品编号规格价格数量收藏夹

ISG15 抗体 (F-9)

sc-166755
200 µg/ml
RMB2377.00

ISG15 (F-9): m-IgG Fc BP-HRP 套装

sc-529040
200 µg Ab; 10 µg BP
RMB2662.00

ISG15 (F-9): m-IgGκ BP-HRP 套装

sc-521732
200 µg Ab, 40 µg BP
RMB2662.00

ISG15 (F-9): m-IgG1 BP-HRP 套装

sc-543284
200 µg Ab; 20 µg BP
RMB2662.00

ISG15 抗体 (F-9) AC

sc-166755 AC
500 µg/ml, 25% agarose
RMB3129.00

ISG15 抗体 (F-9) HRP

sc-166755 HRP
200 µg/ml
RMB2377.00

ISG15 抗体 (F-9) FITC

sc-166755 FITC
200 µg/ml
RMB2482.00

ISG15 抗体 (F-9) PE

sc-166755 PE
200 µg/ml
RMB2580.00

ISG15 抗体 (F-9) Alexa Fluor® 488

sc-166755 AF488
200 µg/ml
RMB2685.00

ISG15 抗体 (F-9) Alexa Fluor® 546

sc-166755 AF546
200 µg/ml
RMB2685.00

ISG15 抗体 (F-9) Alexa Fluor® 594

sc-166755 AF594
200 µg/ml
RMB2685.00

ISG15 抗体 (F-9) Alexa Fluor® 647

sc-166755 AF647
200 µg/ml
RMB2685.00

ISG15 抗体 (F-9) Alexa Fluor® 680

sc-166755 AF680
200 µg/ml
RMB2685.00

ISG15 抗体 (F-9) Alexa Fluor® 790

sc-166755 AF790
200 µg/ml
RMB2685.00

-The datasheet specifies this is "a mouse monoclonal antibody raised against amino acids 1-150" but ISG15 is 165 aa. Is there more information on the epitope (not highlighted in the protein sequence tab)? -5% milk in TBS 0.1% Tween 4 C overnighincubation?

Asked by: autumnos
Thank you for your question. Yes, this information is correct, the antibody is raised against a recombinant protein corresponding to amino acids 1-150 of ISG15 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. We do recommend blocking in 5% nonfat milk in TBST (0.05% Tween-20) for one hour at room temperature, and incubating the primary antibody at the starting dilution of 1:200 in 5% nonfat milk + TBST for one hour at room temperature, or overnight at 4C.
Answered by: Technical Support
Date published: 2019-07-03

What is the light chain of ISG15 Antibody (F-9): sc-166755 ?

Asked by: cjMara
ISG15 Antibody (F-9) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 9
Date published: 2017-09-13
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Rated 5 out of 5 by from Ottimo funzionante ne vorrei altri 100Articolo molto buono ne vorrei molti altri ..............,
Date published: 2024-06-16
Rated 1 out of 5 by from Not working for WB at all1:500 dilution antibody for HAP1 lysate did not work. no thing! I shouldn't order Santa Cruz antibodies again.
Date published: 2023-12-14
Rated 5 out of 5 by from Good antibodyThis antibody works well when tested in western blot of Namalwa whole cell extracts.
Date published: 2022-02-18
Rated 4 out of 5 by from Very good ISG15 antibodyIt works really well, single band, strong. No background. Samples are liver homogenates from bacteria-infected mice.
Date published: 2021-06-04
Rated 4 out of 5 by from Works in WesternClean and sharp band observed by Western blotting at expected height; worked well in lysates of various cell lines; as expected, higher expression upon IFN stimulation
Date published: 2020-09-28
Rated 5 out of 5 by from The antibody is goodI got a free test antibody, the antibody is very good, it also can be used for IP.
Date published: 2019-02-26
Rated 5 out of 5 by from Strong signalF-9 antibody produces a strong signal on WB at 1:1000 dilution. The band observed is a bit "lighter" than expected but that's probably caused by using Tris-Glycine system.
Date published: 2018-02-02
Rated 5 out of 5 by from sc-166755This antibody works great for WB (cell lysates; mouse)
Date published: 2018-01-02
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ISG15 抗体 (F-9) is rated 4.4 out of 5 by 27.
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