Date published: 2026-10-24

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
IRAK-1 Antibody (C-2) - Western Blotting - Image 351974
IRAK-1 抗体 (C-2): sc-5287. 对 HeLa 核提取物 (A) 和 SK-BR-3 (B), SK-N-MC (C), THP-1 (D) 和 Jurkat (E) 全细胞裂解液中 IRAK-1 的表达进行 Western 印迹分析.
IRAK-1 抗体 (C-2): sc-5287. 对 HeLa 核提取物 (A) 和 SK-BR-3 (B), SK-N-MC (C), THP-1 (D) 和 Jurkat (E) 全细胞裂解液中 IRAK-1 的表达进行 Western 印迹分析.

IRAK-1 抗体 (C-2): sc-5287

4.7(6)
寫評論提問
说明书
  • IRAK-1抗体(C-2)是小鼠单克隆IgG2a κ, 在8篇文献中引用，规格为200 µg/ml
  • 免疫human物种的IRAK-1的氨基酸440-712
  • 推荐用于 human 来源的IRAK-1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 IRAK-1 (F-4): sc-5288，可查看有标记的 IRAK-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是IRAK-1 Antibody (C-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与IRAK-1 Antibody (C-2) 打包提供（请参阅下面的订购信息）。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外IRAK抗体，包括IRAK-1, IRAK-2, IRAK-4和IRAK-M

三种结构相关的IL-1R配体已被描述。其中包括两种激动剂IL-1α和IL-1β，以及一种特异性受体拮抗剂IL-1Rα。IL-1RI和IL-1RII两种不同的受体已经被鉴定出来，它们均属于Ig超级家族。大量证据表明IL-1RI是功能性IL-1受体。IL-1与其同源受体结合会激活NFkB信号通路。IL-1依赖激酶IRAK (IL-1受体相关激酶)与活化的IL-1RI共免疫沉淀，并被认为是NFkB活化的上游介质。另外一个与之相关的果蝇蛋白Pelle是NFkB的果蝇同源物Dorsal的上游激活因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRAK-1 抗体 (C-2) 参考文献:

  1. 反义IRAK-1寡核苷酸阻断IL-18诱导的NF-κB和AP-1的激活。  |  Guo, F. and Wu, S. 2000. Immunopharmacology. 49: 241-6. PMID: 10996021
  2. IRAK-4依赖性IRAK-1降解是TLR介导的NF-κB激活的负反馈信号。  |  Kubo-Murai, M., et al. 2008. J Biochem. 143: 295-302. PMID: 18079163
  3. 白细胞介素1受体相关激酶（IRAK）-1介导的NF-κB激活需要细胞质和核活性。  |  Liu, G., et al. 2008. FASEB J. 22: 2285-96. PMID: 18276832
  4. 白细胞介素-1 受体和收费样受体介导的 NF-kappaB 激活需要与 Lys63 链接的 IRAK-1 多泛素化。  |  Conze, DB., et al. 2008. Mol Cell Biol. 28: 3538-47. PMID: 18347055
  5. 1,3,5-三羟基-4-异戊烯基黄酮通过阻碍 IRAK-1 的翻译后修饰抑制脂多糖诱导的 iNOS 表达。  |  Chiou, WF., et al. 2011. Biochem Pharmacol. 81: 752-60. PMID: 21232528
  6. 通过蛋白酶体依赖性下调 TRAF6，IRAK-1 介导了对 Toll 样受体信号的负调控。  |  Muroi, M. and Tanamoto, K. 2012. Biochim Biophys Acta. 1823: 255-63. PMID: 22033459
  7. 白细胞介素-1受体相关激酶1/4（IRAK-1/4）的高选择性抑制剂明确了IRAK-1/4和TAK1激酶的不同信号作用。  |  Scarneo, SA., et al. 2020. J Biol Chem. 295: 1565-1574. PMID: 31914413
  8. 通过抑制TLR4/NF-κB通路和保护肠道屏障，抑制IRAK 1/4可减轻结肠炎。  |  Yan, B., et al. 2022. Bosn J Basic Med Sci. 22: 872-881. PMID: 35699749
  9. IRAK-1和-4双重抑制剂对TLR信号的抑制降低了TPF抗性诱导的HNSCC促致癌效应。  |  Khan, H., et al. 2023. 3 Biotech. 13: 14. PMID: 36540413
  10. 青蒿素通过抑制IRAK-4和IRAK-1，减轻了围产期炎症和少突胶质细胞前体细胞中的氧化应激。  |  Zhang, F., et al. 2024. Int Immunopharmacol. 142: 113117. PMID: 39293313

订购信息

产品名称产品编号规格价格数量收藏夹

IRAK-1 抗体 (C-2)

sc-5287
200 µg/ml
CNY2422.00

IRAK-1 (C-2): m-IgG Fc BP-HRP 套装

sc-536547
200 µg Ab; 10 µg BP
CNY2715.00

IRAK-1 (C-2): m-IgGκ BP-HRP 套装

sc-533698
200 µg Ab; 40 µg BP
CNY2715.00

IRAK-1 (C-2): m-IgG2a BP-HRP 套装

sc-547834
200 µg Ab; 10 µg BP
CNY2715.00

I am using IRAK-1 (C-2): sc-5287 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_10, d_24, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_5287, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by Randy McDonald from Nice antibody for IRAK1 detection in HeLa cellsWe used this antibody for expression detection of IRAK1 by western blot. We got a very nice signal around the correct molecular weight in HeLa cells.
Date published: 2017-02-09
Rated 5 out of 5 by JM from Superb immunoperoxidase cytoplasmic and membraneSuperb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by CJ from Great band detected in WB using T24 wholeGreat band detected in WB using T24 whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by MS from Various publishable data in WB using humanVarious publishable data in WB using human Mono Mac 6 cells, Monocyte, HPC-4, DeTa, HCV29, A549, Jurkat, Raji, HAE, breast carcinoma, pancreatic adenocarcinoma cells and normal bronchial epithelium cells -SCBT Publication Review
Date published: 2015-04-04
Rated 5 out of 5 by TS from Strong band seen in WB using KStrong band seen in WB using K-562 nuclear extract. -SCBT QC
Date published: 2015-01-13
Rated 4 out of 5 by LF from Publishable data in WB using mouse transfectedPublishable data in WB using mouse transfected peritoneal macrophages -SCBT Publication Review
Date published: 2015-01-11
  • y_2026, m_10, d_24, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_5287, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
IRAK-1 抗体 (C-2) is rated 4.7 out of 5 by 6.
  • y_2026, m_10, d_24, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_5287, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 134ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。