Date published: 2026-10-24

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IRAK-1 抗体 (B-5): sc-55530

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说明书
  • IRAK-1抗体(B-5)是小鼠单克隆IgG2b κ, 在7篇文献中引用,规格为200 µg/ml
  • 免疫human来源IRAK-1位于C-terminus 的440-712氨基酸
  • 推荐用于 human 来源的IRAK-1 WB, IP, IF 和 ELISA检测
  • 看 IRAK-1 (F-4): sc-5288,可查看有标记的 IRAK-1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是IRAK-1 Antibody (B-5) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与IRAK-1 Antibody (B-5) 打包提供(请参阅下面的订购信息)。
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已经描述了三种与IL-1R相关的配体。 其中包括两种激动剂IL-1a和IL-1b,以及一种特异性受体拮抗剂IL-1Ra。 已经鉴定出两种不同的受体,称为IL-1RI和IL-1RII,每种受体都属于Ig超家族。 大多数证据表明IL-1RI是功能性IL-1受体。 IL-1与其同源受体的结合导致NFkB信号通路的激活。 IL-1依赖性激酶称为IRAK-1(IL-1受体相关激酶),与活化的IL-1RI共免疫沉淀,并被认为是一种NFkB激活的上游介质。 这一论断的进一步支持来自一个事实,即相关的果蝇蛋白Pelle是已知的果蝇NFkB同源物Dorsal的上游激活剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRAK-1 抗体 (B-5) 参考文献:

  1. 白细胞介素-1受体相关激酶-1(IRAK-1)向细胞核的转移。  |  Böl, G., et al. 2000. FEBS Lett. 477: 73-8. PMID: 10899313
  2. IRAK-1的死亡结构域:介导与MyD88、Tollip、IRAK-1和IRAK-4相互作用的寡聚化结构域。  |  Neumann, D., et al. 2007. Biochem Biophys Res Commun. 354: 1089-94. PMID: 17276401
  3. 利什曼病诱导的 IRAK-1 失活是由 SHP-1 与进化保守的 KTIM 基团相互作用介导的。  |  Abu-Dayyeh, I., et al. 2008. PLoS Negl Trop Dis. 2: e305. PMID: 19104650
  4. 通过C-末端调节IRAK-1的激活。  |  Nguyen, T., et al. 2009. Cell Signal. 21: 719-26. PMID: 19166926
  5. 在硫酸葡聚糖钠诱导的结肠炎中,IRAK-1对性别具有依赖性。  |  Berglund, M., et al. 2009. Cell Immunol. 259: 27-32. PMID: 19540456
  6. IRAK-1通过诱导NOX-1转录和Rac1激活并抑制抗氧化酶的表达,促进巨噬细胞中脂多糖诱导的活性氧的产生。  |  Maitra, U., et al. 2009. J Biol Chem. 284: 35403-11. PMID: 19850916
  7. 白细胞介素-1受体相关激酶-1(IRAK-1)在巨噬细胞迁移的调节中与PKCepsilon和VASP功能相关。  |  Gan, L. and Li, L. 2010. Mol Immunol. 47: 1278-82. PMID: 20044140
  8. 微RNA-146a和NF-kappaB对受压人类星状胶质细胞和阿尔茨海默病中白细胞介素-1受体相关激酶-1(IRAK-1)和IRAK-2的不同调控作用  |  Cui, JG., et al. 2010. J Biol Chem. 285: 38951-60. PMID: 20937840
  9. 白介素-1受体相关激酶(IRAK)-1的表达增加与肥胖症中的脂肪组织炎症状态有关。  |  Ahmad, R., et al. 2015. Diabetol Metab Syndr. 7: 71. PMID: 26312071
  10. 遗传性人类 IRAK-1 缺乏症会选择性地损害成纤维细胞中的 TLR 信号转导。  |  Della Mina, E., et al. 2017. Proc Natl Acad Sci U S A. 114: E514-E523. PMID: 28069966

订购信息

产品名称产品编号规格价格数量收藏夹

IRAK-1 抗体 (B-5)

sc-55530
200 µg/ml
CNY2422.00

IRAK-1 (B-5): m-IgG Fc BP-HRP 套装

sc-537064
200 µg Ab; 10 µg BP
CNY2715.00

IRAK-1 (B-5): m-IgGκ BP-HRP 套装

sc-534250
200 µg Ab; 40 µg BP
CNY2715.00

What is the recommended starting dilution for Western blot for sc-55530: IRAK-1 (B-5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by Randy McDonald from Great for western blotWe used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 4 out of 5 by HC from Publishable data in WB using human brainPublishable data in WB using human brain tissue and cells. -SCBT Publication Review
Date published: 2015-05-13
Rated 4 out of 5 by MC from Publishable data in IP using mouse RAW264Publishable data in IP using mouse RAW264.7 and peritoneal macrophages cells -SCBT Publication Review
Date published: 2015-05-04
Rated 4 out of 5 by HC from Robust bands seen in WB using HeLa and PMARobust bands seen in WB using HeLa and PMA treated K-562 whole cell lysates and K-562 and PMA treated K-562 nuclear extracts. -SCBT QC
Date published: 2015-03-10
Rated 4 out of 5 by ET from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by JM from Publishable data in WB using mouse RAW264Publishable data in WB using mouse RAW264.7 and peritoneal macrophages cells -SCBT Publication Review
Date published: 2013-12-30
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IRAK-1 抗体 (B-5) is rated 4.3 out of 5 by 6.
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