Date published: 2025-9-7
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IQGAP1 Antibody (C-9) - Western Blotting - Image 355832
IQGAP1 抗体 (C-9): sc-376021. KNRK (A), A549 (B), NIH/3T3 (C), F9 (D), RAW 264. 7 (E) 和 MDA-MB-231 (F) 全细胞裂解液中 IQGAP1 表达的 Western 印迹分析.
IQGAP1 抗体 (C-9): sc-376021. KNRK (A), A549 (B), NIH/3T3 (C), F9 (D), RAW 264. 7 (E) 和 MDA-MB-231 (F) 全细胞裂解液中 IQGAP1 表达的 Western 印迹分析.

IQGAP1 抗体 (C-9): sc-376021

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说明书
  • IQGAP1 抗体 C-9 是小鼠单克隆 IgG1 κ，IQGAP1抗体, 在26篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的IQGAP1的N-terminus附近的氨基酸3-37之间
  • IQGAP1 抗体 (C-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IQGAP1; 也和以下物种反应，包括： canine
  • 抗IQGAP1抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IQGAP1 (C-9): sc-376021的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是IQGAP1 Antibody (C-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与IQGAP1 Antibody (C-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外IQGAP抗体，包括IQGAP1, IQGAP2和IQGAP3

IQGAP1抗体（C-9）是一种IgG1 κ小鼠单克隆IQGAP1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的IQGAP1蛋白。IQGAP1抗体（C-9）既可以非共轭抗IQGAP1抗体的形式提供，也可以多种共轭形式的抗IQGAP1抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®共轭物。IQGAP1，即含有IQ基序的GTP酶激活蛋白，是一种与Ras GAP相关、与肌动蛋白结合的蛋白，与小GTP酶Cdc42和Rac1相互作用。IQGAP1的C末端对于与Cdc42的相互作用至关重要。此外，IQGAP1还包含一个WW结构域和一个预测的N末端卷曲螺旋区域，这可能参与IQGAP二聚化。IQGAP1在胎盘、肺和肾中的表达量最高，在这些组织中，它与Cdc42共同定位到细胞骨架，并协助Cdc42介导细胞增殖、极性和细胞形态的调节。IQGAP1通过与E-钙粘蛋白、β-连环蛋白和α-连环蛋白结合来调节钙粘蛋白介导的细胞粘附。这种关联诱导这些蛋白在细胞-细胞接触部位的积累。IQGAP1受到钙调蛋白的负调控，钙调蛋白以钙依赖的方式与IQGAP1结合，并破坏IQGAP1与Cdc42的关联。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IQGAP1 抗体 (C-9) 参考文献:

  1. 鉴定含有钙调蛋白结合基团的人类 rasGAP 相关蛋白。  |  Weissbach, L., et al. 1994. J Biol Chem. 269: 20517-21. PMID: 8051149
  2. 确定 IQGAP 为小 GTP 酶 Cdc42 和 Rac1 的假定靶标。  |  Kuroda, S., et al. 1996. J Biol Chem. 271: 23363-7. PMID: 8798539
  3. 钙调蛋白调节 IQGAP1 和 Cdc42 之间的相互作用。通过纳米电喷雾串联质谱鉴定 IQGAP1。  |  Joyal, JL., et al. 1997. J Biol Chem. 272: 15419-25. PMID: 9182573
  4. IQGAP1 是一种 Rac 和 Cdc42 结合蛋白，可直接与微丝结合并交联。  |  Bashour, AM., et al. 1997. J Cell Biol. 137: 1555-66. PMID: 9199170
  5. 鉴定包括 IQGAP 和 Cdc42 GTPase 的肌动蛋白细胞骨架复合体。  |  Erickson, JW., et al. 1997. J Biol Chem. 272: 24443-7. PMID: 9305904
  6. 肌动蛋白结合蛋白 IQGAP 和活化的 Cdc42 与高尔基体膜的缔合作用特征。  |  McCallum, SJ., et al. 1998. J Biol Chem. 273: 22537-44. PMID: 9712880
  7. IQGAP 相关蛋白 DGAP1 与 Rac 相互作用，参与调节 F-肌动蛋白细胞骨架和控制细胞运动。  |  Faix, J., et al. 1998. J Cell Sci. 111 (Pt 20): 3059-71. PMID: 9739079

订购信息

产品名称产品编号规格价格数量收藏夹

IQGAP1 抗体 (C-9)

sc-376021
200 µg/ml
RMB2377.00

IQGAP1 (C-9): m-IgG Fc BP-HRP 套装

sc-529803
200 µg Ab; 10 µg BP
RMB2662.00

IQGAP1 (C-9): m-IgGκ BP-HRP 套装

sc-522820
200 µg Ab, 40 µg BP
RMB2662.00

IQGAP1 (C-9): m-IgG1 BP-HRP 套装

sc-543696
200 µg Ab; 20 µg BP
RMB2662.00

IQGAP1 抗体 (C-9) AC

sc-376021 AC
500 µg/ml, 25% agarose
RMB3129.00

IQGAP1 抗体 (C-9) HRP

sc-376021 HRP
200 µg/ml
RMB2377.00

IQGAP1 抗体 (C-9) FITC

sc-376021 FITC
200 µg/ml
RMB2482.00

IQGAP1 抗体 (C-9) PE

sc-376021 PE
200 µg/ml
RMB2580.00

IQGAP1 抗体 (C-9) Alexa Fluor® 488

sc-376021 AF488
200 µg/ml
RMB2685.00

IQGAP1 抗体 (C-9) Alexa Fluor® 546

sc-376021 AF546
200 µg/ml
RMB2685.00

IQGAP1 抗体 (C-9) Alexa Fluor® 594

sc-376021 AF594
200 µg/ml
RMB2685.00

IQGAP1 抗体 (C-9) Alexa Fluor® 647

sc-376021 AF647
200 µg/ml
RMB2685.00

IQGAP1 抗体 (C-9) Alexa Fluor® 680

sc-376021 AF680
200 µg/ml
RMB2685.00

IQGAP1 抗体 (C-9) Alexa Fluor® 790

sc-376021 AF790
200 µg/ml
RMB2685.00

IQGAP1 (C-9) 中和勝肽

sc-376021 P
100 µg/0.5 ml
RMB511.00

Does this antibody can bind to Streptavidin Magarose Beads for IP (immunoprecipitation) experiments？

Asked by: patty97
Thank you for contacting Santa Cruz Biotechnology. Please contact Technical Service for assistance. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-05-22

Can this mouse monoclonal antibody be used to stain mouse tissue in IHC?

Asked by: AbPolly
Thank you for your question. Yes, we recommend using a directly conjugated mouse monoclonal antibody. This antibody is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 7
Date published: 2017-02-09
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Rated 3 out of 5 by from WBWe tried this Ab in Hela cells and we saw low quality signal
Date published: 2020-12-02
Rated 4 out of 5 by from very good antibodyI used it for immunoprecipitation from different human cancer cell lines and I got very clean and strong signal. I also used it for western blotting to detect Iqgap1 in the same cell lines. In 1:1000 dilution, it gave very strong signal.
Date published: 2017-10-16
Rated 5 out of 5 by from works great on western blot and IHCWe have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 4 out of 5 by from Seemed good for western blottingI used mouse Hepa1-6 cell lysates with loading gradient to check this antibody in western blotting. Antibody was diluted 1000X and incubated at 4℃ overnight. The exposure results showed several bands from 130-170 KD . But I didn’t use si specific genes to check whether this band was specific.
Date published: 2017-08-18
Rated 5 out of 5 by from Excellent antibodyThis is one of the best antibody that I worked so far, it worked perfectly on western and IF on paraffin embedded mouse tissue.
Date published: 2017-04-03
Rated 4 out of 5 by from Great Ab!Works well for western blotting (mouse samples)!!!
Date published: 2017-03-29
Rated 5 out of 5 by from gratefully workedOverexpressed GFP-IQ1 (human clone) in 293 cells was detected. endogenous IQ1 also was detected. overexpressed IQ2 and IQ3 were not detected. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-23
Rated 5 out of 5 by from Great detection.A great choice for detecting the mouse, rat, or human protein. It works well in standard applications and will provide you clear bands and specific data. This product has been published with several times.
Date published: 2017-01-27
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IQGAP1 抗体 (C-9) is rated 4.4 out of 5 by 12.
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