Date published: 2025-9-8

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INSR/Insulin Receptor α 抗体 (MA-20): sc-57344

4.5(4)
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说明书
  • INSR/Insulin Receptor α抗体(MA-20)是小鼠单克隆IgG2b κ, 在14篇文献中引用,规格为200 µg/ml
  • 针对胎盘(insulin Rα,human)
  • 推荐用于 mouse, rat 和 human 来源的insulin Rα WB 和 IP检测
  • 抗INSR/Insulin Receptor α抗体(MA-20)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取INSR/Insulin Receptor α (MA-20): sc-57344免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成INSR/Insulin Receptor α 抗体 (MA-20)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

胰岛素 Rα 抗体 (MA-20) 是一种小鼠单克隆 IgG2b kappa 轻链抗体,专门设计用于在免疫沉淀 (IP) 应用中靶向人源胰岛素受体α亚基(胰岛素 Rα)。胰岛素 Rα 单克隆抗体 (MA-20) 是针对人类胎盘胰岛素 Rα 制备的,可精确检测人类样本中的胰岛素受体,并与小鼠和大鼠的胰岛素 Rα 发生交叉反应,为涉及这些模式生物的研究提供了多功能性。胰岛素受体是一种重要的异源二聚体蛋白,由两个胞外α亚基和两个跨膜β亚基通过二硫键连接而成。α亚基负责与胰岛素结合,从而触发一系列细胞内信号通路,调节葡萄糖摄取、糖原合成和整个细胞代谢。胰岛素 Rα 的翻译后修饰,特别是 N-连接糖基化,对于蛋白质的正确折叠、稳定性和细胞表面定位至关重要。这些糖基化事件有助于受体的正确组装和二聚化。此外,糖基化还影响受体与其他细胞蛋白的相互作用以及胰岛素的反应能力,在维持代谢平衡方面起着关键作用。这些翻译后修饰的失调会导致胰岛素信号传导受损,从而引发胰岛素抵抗和 2 型糖尿病。胰岛素 Rα (MA-20) 单克隆抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物,可在不同的实验装置中灵活使用。抗胰岛素 Rα 抗体 (MA-20) 推荐用于研究胰岛素作用和糖尿病病理生理学的分子基础。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

INSR/Insulin Receptor α 抗体 (MA-20)

sc-57344
200 µg/ml
RMB2377.00

INSR/Insulin Receptor α (MA-20): m-IgG Fc BP-HRP 套装

sc-551776
200 µg Ab; 10 µg BP
RMB2662.00

INSR/Insulin Receptor α 抗体 (MA-20) AC

sc-57344 AC
500 µg/ml, 25% agarose
RMB3129.00

INSR/Insulin Receptor α 抗体 (MA-20) HRP

sc-57344 HRP
200 µg/ml
RMB2377.00

INSR/Insulin Receptor α 抗体 (MA-20) FITC

sc-57344 FITC
200 µg/ml
RMB2482.00

INSR/Insulin Receptor α 抗体 (MA-20) PE

sc-57344 PE
200 µg/ml
RMB2580.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 488

sc-57344 AF488
200 µg/ml
RMB2685.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 546

sc-57344 AF546
200 µg/ml
RMB2685.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 594

sc-57344 AF594
200 µg/ml
RMB2685.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 647

sc-57344 AF647
200 µg/ml
RMB2685.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 680

sc-57344 AF680
200 µg/ml
RMB2685.00

INSR/Insulin Receptor α 抗体 (MA-20) Alexa Fluor® 790

sc-57344 AF790
200 µg/ml
RMB2685.00

To which domain does INSR/Insulin Receptor α Antibody (MA-20): sc-57344 bind to the insulin receptor: the extracellular domain alpha domain (n-terminus)  the intracellular domain or both.  

Asked by: Geert
Thank you for your question. The antibody binds to the extracellular domain.
Answered by: BlakeJ
Date published: 2023-06-16

Are there any issues with using sc-57344: insulin Rα (MA-20) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-57344 conjugates.
Answered by: Technical Support
Date published: 2023-06-10
  • y_2025, m_9, d_7, h_4CST
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Rated 4 out of 5 by from アプリケーションノート通りウェスタンブロッティングで使えるかとラットの骨格筋組織ライゼートで試してみましたが、やはりダメでした。
Date published: 2022-03-24
Rated 5 out of 5 by from Got target bandWe tried the antibody dilution range from 1:300 to 1:800.We can get 125kda target band, The high conceration 1:300 may cause background and non specific bands, I suggested that the concentration is around 1:500, the exposure time should not be too long, we recommend controled in 3min,also I highly recommend do internal control on the same membrance at the same time to test the experiment conditions.
Date published: 2017-09-10
Rated 5 out of 5 by from Popular antibody!This is a popular clone for insulin R alpha detection. Great choice!
Date published: 2017-02-07
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INSR/Insulin Receptor α 抗体 (MA-20) is rated 4.5 out of 5 by 4.
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