Date published: 2025-9-7

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IL-8 抗体 (B-2): sc-8427

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说明书
  • IL-8抗体(B-2)是小鼠单克隆IgG2b κ, 在36篇文献中引用,规格为200 µg/ml
  • 免疫human物种的IL-8的氨基酸40-99
  • IL-8 抗体 (B-2) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 IL-8
  • 抗IL-8抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-8 (B-2): sc-8427免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是IL-8 Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IL-8 Antibody (B-2) 打包提供(请参阅下面的订购信息)。

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関連項目

IL-8抗体(B-2)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附(ELISA)等方法检测人体样本中的白细胞介素-8。IL-8 (B-2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物,可满足不同实验需求。白细胞介素-8(IL-8)是趋化因子C-X-C家族的重要成员,主要作为中性粒细胞的强效趋化因子发挥作用,而中性粒细胞对于免疫反应至关重要。IL-8由多种细胞分泌,包括巨噬细胞和上皮细胞,在炎症和免疫反应中发挥重要作用。值得注意的是,IL-8以99个氨基酸的前体形式产生,然后裂解为72个氨基酸的非糖基化生物活性形式。IL-8单体和二聚体(在溶液中和结合到细胞表面时)之间的动态平衡对于调节趋化和受体信号至关重要。研究表明,IL-8二聚化可作为IL-8受体功能的负调节因子,这对于维持免疫反应的平衡至关重要。IL-8的两个已知受体IL-8RA和IL-8RB具有77%的序列相似性,被归类为七跨膜结构域蛋白,类似于G蛋白偶联受体,能够与其他趋化因子相互作用并调节各种炎症过程。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IL-8 抗体 (B-2)

sc-8427
200 µg/ml
RMB2377.00

IL-8 (B-2): m-IgG Fc BP-HRP 套装

sc-528230
200 µg Ab; 10 µg BP
RMB2662.00

IL-8 (B-2): m-IgGκ BP-HRP 套装

sc-520556
200 µg Ab, 40 µg BP
RMB2662.00

IL-8 (B-2): m-IgG2b BP-HRP 套装

sc-550008
200 µg Ab; 10 µg BP
RMB2662.00

IL-8 抗体 (B-2) AC

sc-8427 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-8 抗体 (B-2) HRP

sc-8427 HRP
200 µg/ml
RMB2377.00

IL-8 抗体 (B-2) FITC

sc-8427 FITC
200 µg/ml
RMB2482.00

IL-8 抗体 (B-2) PE

sc-8427 PE
200 µg/ml
RMB2580.00

IL-8 抗体 (B-2) Alexa Fluor® 488

sc-8427 AF488
200 µg/ml
RMB2685.00

IL-8 抗体 (B-2) Alexa Fluor® 546

sc-8427 AF546
200 µg/ml
RMB2685.00

IL-8 抗体 (B-2) Alexa Fluor® 594

sc-8427 AF594
200 µg/ml
RMB2685.00

IL-8 抗体 (B-2) Alexa Fluor® 647

sc-8427 AF647
200 µg/ml
RMB2685.00

IL-8 抗体 (B-2) Alexa Fluor® 680

sc-8427 AF680
200 µg/ml
RMB2685.00

IL-8 抗体 (B-2) Alexa Fluor® 790

sc-8427 AF790
200 µg/ml
RMB2685.00

Hi there, Is this antibody and CXCL12/SDF-1α (F-4) (Catalog number: sc-518066) sodium azide free

Asked by: Sultana
Thank you for your question. Our standard buffer format for these antibodies contain sodium azide as a preservative. The antibodies are provided in a buffer of PBS with < 0.1% sodium azide and 0.1% gelatin. Please contact our Special Orders Department to inquire about availability and to receive a quote for an azide-free preparation of our antibodies. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2020-03-25

HI, May a have a sample of  IL-8 (B-2): sc-8427. I am a graduate student at the University of Arizona. 

Asked by: AndreaCas
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample, e-mail <[email protected]>.
Answered by: Technical Service 11
Date published: 2018-01-09

Is this antibody appropriate for use in IHC with paraffin-embedded sections?

Asked by: TinTin
Thank you for your question. While this antibody has not been validated for use with paraffin-embedded samples by our labs, there are publications siting this antibody for use in IHC. Please contact our Technical Service Department for further details and recommendations.
Answered by: Tech Service
Date published: 2017-01-30
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Rated 5 out of 5 by from Perfect antibodyWe used this antibody for IL-8 estimation by ELISA. Highly recommended for ELISA and western blotting.
Date published: 2019-12-04
Rated 4 out of 5 by from Nice fair band in WBUsed for WB dilution 1/1000 for detection of IL8 in total lysates and conditioned medium after concentration
Date published: 2019-05-16
Rated 5 out of 5 by from nice and clear bandsit gives strong and clear bands where expected, it is our preference to use this antibody
Date published: 2019-01-24
Rated 3 out of 5 by from not very well for Weatern Blotnot very well for Weatern Blot. It was not a single band for weatern blot in MCF-7 cell by diluting 1:1000 overnight in 4℃.
Date published: 2018-04-17
Rated 1 out of 5 by from bad detection on total extractWe tested this antibody on RIPA extracts and obtained no results even on control lines. it seems that this antibody is more useful for the detection of the recombinant protein
Date published: 2018-04-04
Rated 5 out of 5 by from Positive signal via western blot analysisWB using human recombinant IL-8 fusion protein, shows powerful band.
Date published: 2017-04-22
Rated 5 out of 5 by from Great results for WB and IHCHighly recommended with lots of references in the primary literature makes this IL-8 antibody a solid choice for WB and IHC
Date published: 2017-01-07
Rated 5 out of 5 by from Worked for western blotting with HMVECs and CXWorked for western blotting with HMVECs and CX-1NS lysates. -SCBT Publication Review
Date published: 2015-04-07
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IL-8 抗体 (B-2) is rated 4.4 out of 5 by 12.
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