Date published: 2025-9-7

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IL-7 抗体 (D-9): sc-365306

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说明书
  • IL-7 抗体 D-9 是小鼠单克隆 IgG1 κ,IL-7抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的IL-7的氨基酸27-177
  • IL-7 抗体 (D-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 IL-7
  • 抗IL-7抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-7 (D-9): sc-365306免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 IL-7 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IL-7 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

IL-7抗体(D-9)是一种IgG1 κ小鼠单克隆IL-7抗体(也称为IL-7抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源IL-7蛋白。IL-7抗体(D-9)既可作为非偶联抗IL-7抗体形式,也可作为多种偶联形式的抗IL-7抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。白细胞介素-7(IL-7)最初被描述为一种能够在体外诱导骨髓培养中前B细胞增殖的因子。IL-7基因编码长度为177个氨基酸的蛋白质。IL-7通过IL-7受体发挥其生物学功能,该受体表达于前B细胞、胸腺细胞和骨髓源性巨噬细胞上。IL-7受体由IL-7受体特异性链和IL-2、IL-4、IL-7、IL-9和IL-15受体共有的IL-2受体γ链组成。IL-7刺激导致Janus酪氨酸激酶家族成员JAK1和JAK3的激活。其他研究表明,在T细胞中,IL-7受体特异性链与Src激酶家族的Lck和Fyn相关。IL-7诱导胰岛素受体底物-1(IRS-1)和胰岛素受体底物-2(IRS-2)的磷酸化,最初称为4PS。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-7 抗体 (D-9) 参考文献:

  1. 抗细胞因子单克隆抗体的开发策略:临床样本中 IL-10 和 IL-5 的免疫测定。  |  Abrams, JS., et al. 1992. Immunol Rev. 127: 5-24. PMID: 1387110
  2. 克隆人类 IL-2 受体的 gamma 链。  |  Takeshita, T., et al. 1992. Science. 257: 379-82. PMID: 1631559
  3. 人类和鼠类白细胞介素-7 受体的克隆:证明了一种可溶性形式和新受体超家族的同源性。  |  Goodwin, RG., et al. 1990. Cell. 60: 941-51. PMID: 2317865
  4. 小鼠白细胞介素-4 受体:分泌型和膜结合型的分子克隆和特征描述。  |  Mosley, B., et al. 1989. Cell. 59: 335-48. PMID: 2805066
  5. IL-7 在恶性间皮瘤中表达,具有预后价值。  |  Mai, HL., et al. 2022. Mol Oncol. 16: 3606-3619. PMID: 36054746
  6. IL-7 缺乏会加剧 NC/Nga 小鼠的特应性皮炎  |  Park, HJ., et al. 2023. Int J Mol Sci. 24: PMID: 37373104
  7. 工程化 IL-7 与 IL-12 免疫疗法协同作用,可防止 T 细胞衰竭并促进记忆,同时不会加剧毒性。  |  Kang, S., et al. 2023. Sci Adv. 9: eadh9879. PMID: 38019919
  8. 长期培养小鼠骨髓中的 B 淋巴细胞及其前体。  |  Whitlock, CA. and Witte, ON. 1982. Proc Natl Acad Sci U S A. 79: 3608-12. PMID: 6980414
  9. IL-2 和 IL-4 的受体共享白细胞介素-2(IL-2)受体 gamma 链。  |  Kondo, M., et al. 1993. Science. 262: 1874-7. PMID: 8266076
  10. 白细胞介素-2 受体伽马链:白细胞介素-4 受体的功能成分。  |  Russell, SM., et al. 1993. Science. 262: 1880-3. PMID: 8266078
  11. 小鼠白细胞介素 2 受体(IL-2R)γ 链 cDNA 编码的特征:IL-2R γ 链表达的染色体图谱和组织特异性。  |  Cao, X., et al. 1993. Proc Natl Acad Sci U S A. 90: 8464-8. PMID: 8378320
  12. IL-2 受体复合物:其结构, 功能和靶基因。  |  Minami, Y., et al. 1993. Annu Rev Immunol. 11: 245-68. PMID: 8476561

订购信息

产品名称产品编号规格价格数量收藏夹

IL-7 抗体 (D-9)

sc-365306
200 µg/ml
RMB2377.00

IL-7 (D-9): m-IgG Fc BP-HRP 套装

sc-527352
200 µg Ab; 10 µg BP
RMB2662.00

IL-7 (D-9): m-IgG1 BP-HRP 套装

sc-532725
200 µg Ab; 20 µg BP
RMB2662.00

IL-7 抗体 (D-9) AC

sc-365306 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-7 抗体 (D-9) HRP

sc-365306 HRP
200 µg/ml
RMB2377.00

IL-7 抗体 (D-9) FITC

sc-365306 FITC
200 µg/ml
RMB2482.00

IL-7 抗体 (D-9) PE

sc-365306 PE
200 µg/ml
RMB2580.00

IL-7 抗体 (D-9) Alexa Fluor® 488

sc-365306 AF488
200 µg/ml
RMB2685.00

IL-7 抗体 (D-9) Alexa Fluor® 546

sc-365306 AF546
200 µg/ml
RMB2685.00

IL-7 抗体 (D-9) Alexa Fluor® 594

sc-365306 AF594
200 µg/ml
RMB2685.00

IL-7 抗体 (D-9) Alexa Fluor® 647

sc-365306 AF647
200 µg/ml
RMB2685.00

IL-7 抗体 (D-9) Alexa Fluor® 680

sc-365306 AF680
200 µg/ml
RMB2685.00

IL-7 抗体 (D-9) Alexa Fluor® 790

sc-365306 AF790
200 µg/ml
RMB2685.00

Hello, kindly I would like to know what does it mean "fusion protein" why in WB we can identifies 3 bands from different molecular weight? are those bands IL-7 ? Thanks a lot

Asked by: Carlos99
Thank you for your question. On this antibody's product page we show a western blot image of full length human recombinant IL-7 fusion protein. This protein consists of a peptide corresponding to amino acids 27-177 of IL-7 of human origin, and several different marker proteins. In western blots with regular cell lysates the expected molecular weight is 25 kDa.
Answered by: Tech Support Europe
Date published: 2020-06-15

hi is the antibody can used for detection of IL7 from mouse origin?

Asked by: ali a
Thank you for your question. We have not yet tested this antibody in mouse samples, and so it is not a recommended species at this time.
Answered by: Technical Support
Date published: 2019-05-01

Can this antibody recognize murine IL-7? specifically, detection via IHC or IF? Thanks in advance!

Asked by: katinthehat
Thank you for your question. This antibody is currently only recommended for human use, but it has not been tested on mouse samples. It may work, but can not be recommended for that use. We would be happy to send you a sample of sc-365306 if you wish to try it. Please reach out to Technical support at [email protected], or via chat directly on our website, www.scbt.com, and we would be happy to supply you with a sample.
Answered by: Tech Service
Date published: 2017-12-04

I am using IL-7 (D-9): sc-365306 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great IF staining resultsWe used this antibody to take IF pictures and got very nice images from the binding in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from Good publishable data in WBGood publishable data in WB, Flow and ChIP in A549 and LH7 cell. -SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-01-10
Rated 5 out of 5 by from Robust signal in WB in human recombinant ILRobust signal in WB in human recombinant IL-7 fusion protein. -SCBT QC
Date published: 2015-01-07
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IL-7 抗体 (D-9) is rated 5.0 out of 5 by 4.
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