Date published: 2025-9-8
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IL-6Rα Antibody (H-7) - Western Blotting - Image 375645
IL-6Rα 抗体 (H-7): sc-373708. BJAB (A) 和 NCI-H929 (B) 全细胞裂解液中 IL-6Rα 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
IL-6Rα 抗体 (H-7): sc-373708. BJAB (A) 和 NCI-H929 (B) 全细胞裂解液中 IL-6Rα 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

IL-6Rα 抗体 (H-7): sc-373708

4.8(8)
寫評論提問

说明书
  • IL-6Rα抗体(H-7)是小鼠单克隆IgG1 κ, 在28篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的IL-6Rα的C-terminus的氨基酸441-468之间
  • 抗-IL-6R alpha 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IL-6Rα
  • 抗IL-6R alpha抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-6Rα (H-7): sc-373708的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是IL-6Rα Antibody (H-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与IL-6Rα Antibody (H-7) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加IL-6R抗体，包括IL-6R α

IL-6Rα抗体（H-7）是一种IgG1 κ小鼠单克隆IL-6Rα抗体（也称为IL-6Rα抗体或白介素6受体抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的IL-6Rα。IL-6Rα抗体（H-7）既可以作为非偶联的抗IL-6Rα抗体形式，也可以作为多种偶联形式的抗IL-6Rα抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。IL-6通过与由配体结合蛋白（IL-6Rα）和第二种蛋白质组成的受体结合来激活细胞内信号传导。IL-6首先与IL-6Rα（也称为gp80）结合，随后与gp130二聚体相关联。活性信号复合物至少由IL-6、IL-6Rα和两个通过二硫键连接的gp130蛋白二聚体组成。可溶性形式的IL-6Rα，即sIL-6Rα，是通过膜结合前体的蛋白水解裂解产生的，可以作为激动剂分子积极参与细胞间信号传导。IL-6复合物的第二个亚基gp130也是几种其他受体复合物的组成部分，包括白血病抑制因子（LIF）、抑瘤素M（OSM）、睫状神经营养因子（CNTF）和IL-11。LIF与LIF受体低亲和力结合，与LIF受体和gp130的复合物高亲和力结合，而OSM似乎与gp130低亲和力结合，与gp130和LIF受体的复合物高亲和力结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IL-6Rα 抗体 (H-7)

sc-373708
200 µg/ml
RMB2377.00

IL-6Rα (H-7): m-IgG Fc BP-HRP 套装

sc-525917
200 µg Ab; 10 µg BP
RMB2662.00

IL-6Rα 抗体 (H-7) AC

sc-373708 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-6Rα 抗体 (H-7) HRP

sc-373708 HRP
200 µg/ml
RMB2377.00

IL-6Rα 抗体 (H-7) FITC

sc-373708 FITC
200 µg/ml
RMB2482.00

IL-6Rα 抗体 (H-7) PE

sc-373708 PE
200 µg/ml
RMB2580.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 488

sc-373708 AF488
200 µg/ml
RMB2685.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 546

sc-373708 AF546
200 µg/ml
RMB2685.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 594

sc-373708 AF594
200 µg/ml
RMB2685.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 647

sc-373708 AF647
200 µg/ml
RMB2685.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 680

sc-373708 AF680
200 µg/ml
RMB2685.00

IL-6Rα 抗体 (H-7) Alexa Fluor® 790

sc-373708 AF790
200 µg/ml
RMB2685.00

IL-6Rα (H-7) 中和勝肽

sc-373708 P
100 µg/0.5 ml
RMB511.00

做石蜡切片免疫组化抗原修复用酸修复液还是碱修复液？

Asked by: ANNA
感谢您的提问。酸修复液和碱修复液都可以用于抗原修复。您可以查看我们IHC实验流程进一步了解。实验流程链接如下：https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining Thank you for your question. Both acid and alkali repair solutions can be used for antigen repair. You can check out our IHC experimental procedures for further information. The experimental process link as follows: https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining
Answered by: Zhanglong
Date published: 2024-07-22

Hello, Can I use it for flow cytometry and what dilution do you recomment

Asked by: Researcher 2021
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

Hi, I bought the IL-6Rα (H-7): sc-373708 Ab which I aim to use for IF in fixed cells. What fixation media should I use? Would PFA be ok for this antibody?

Asked by: Athina
Thank you for your question. We suggest acetone or methanol for fixation for IF applications. You can find our recommended protocol here: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2020-01-23

What application is the blocking peptide sc-373708 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 人肿瘤组织的免疫组化也很好使这个抗体用于人肿瘤组织的免疫组化也很好使，，， IHC good ,good good ，good ，good
Date published: 2018-06-06
Rated 4 out of 5 by from good antibodyI used this antibody with a dilution of 1:200 and got a clear band
Date published: 2017-10-23
Rated 4 out of 5 by from IL-6RThere was a band detected at about 80 kd, but a very strong band appeared at 70kd.
Date published: 2017-09-04
Rated 5 out of 5 by from Good antibodyThis one gives a strong band, I would recommend to use.
Date published: 2017-08-24
Rated 5 out of 5 by from IL-6RI have applied for a smaple from santa cruz, and I use it in westernblot which has a clear picture.
Date published: 2017-02-20
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of IL-6Rα expression in HeLa and BJAB whole cell lysates.
Date published: 2017-01-24
Rated 5 out of 5 by from Highly recommendedStrong bands in western blot analysis of IL-6R expression in HepG2 cell lysates.
Date published: 2016-12-18
Rated 5 out of 5 by from Produced nice Western blot data of ILProduced nice Western blot data of IL-6R expression in HeLa and BJAB whole cell lysates. -SCBT QC
Date published: 2013-12-24
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IL-6Rα 抗体 (H-7) is rated 4.8 out of 5 by 8.
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