Date published: 2025-9-7
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IL-6 Antibody (C12-1-hIL-6) - Western Blotting - Image 8857
IL-6 抗体 (C12-1-hIL-6): sc-32296. 人重组 IL-6 融合蛋白的 Western 印迹分析.
IL-6 Antibody (C12-1-hIL-6) - Western Blotting - Image 8857
IL-6 抗体 (C12-1-hIL-6): sc-32296. 人重组 IL-6 融合蛋白的 Western 印迹分析.
IL-6 Antibody (C12-1-hIL-6) - Western Blotting - Image 30168
IL-6 抗体 (C12-1-hIL-6): sc-32296. 未转染: sc-117752 (A) 和转染人 IL-6: sc-113126 (B) 293T 全细胞裂解液中 IL-6 表达的 Western 印迹分析.
IL-6 Antibody (C12-1-hIL-6) - Western Blotting - Image 362187
IL-6 抗体 (C12-1-hIL-6): sc-32296. Western 印迹分析 Neuro-2A 全细胞裂解液中 IL-6 的表达.
IL-6 抗体 (C12-1-hIL-6): sc-32296. 人重组 IL-6 融合蛋白的 Western 印迹分析.

IL-6 抗体 (C12-1-hIL-6): sc-32296

3.6(10)
寫評論提問

说明书
  • IL-6抗体(C12-1-hIL-6)是小鼠单克隆IgG2a κ, 在53篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组IL-6
  • 抗-IL-6 抗体 (C12-1-hIL-6) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 IL-6
  • 抗IL-6抗体（C12-1-hIL-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-6 (C12-1-hIL-6): sc-32296的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是IL-6 Antibody (C12-1-hIL-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IL-6 Antibody (C12-1-hIL-6) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

IL-6 抗体（C12-1-hIL-6）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠和人类样本中的 IL-6。IL-6 (C12-1-hIL-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足不同实验需求。白细胞介素-6（IL-6）是一种由212个氨基酸组成的多功能细胞因子，在免疫反应、炎症和造血中发挥着关键作用。IL-6主要由免疫细胞（包括T细胞和巨噬细胞）分泌，在感染和组织损伤期间对急性期反应的调节至关重要。IL-6能够诱发发烧并刺激肝脏产生急性期蛋白，这凸显了其在宿主防御机制中的重要性。IL-6通过与特定受体结合形成三聚体复合物来发挥其作用，从而激活细胞内信号传导通路，包括JAK/STAT通路，最终影响与免疫和炎症反应相关的基因表达。IL-6的动态调节对于维持体内平衡至关重要，而IL-6信号传导的失调与多种病理状况有关，包括自身免疫性疾病和癌症，这使得IL-6成为治疗干预的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IL-6 抗体 (C12-1-hIL-6)

sc-32296
200 µg/ml
RMB2377.00

IL-6 (C12-1-hIL-6): m-IgG Fc BP-HRP 套装

sc-528455
200 µg Ab; 10 µg BP
RMB2662.00

IL-6 (C12-1-hIL-6): m-IgGκ BP-HRP 套装

sc-520836
200 µg Ab, 40 µg BP
RMB2662.00

IL-6 (C12-1-hIL-6): m-IgG2a BP-HRP 套装

sc-547104
200 µg Ab; 10 µg BP
RMB2662.00

IL-6 抗体 (C12-1-hIL-6) AC

sc-32296 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-6 抗体 (C12-1-hIL-6) HRP

sc-32296 HRP
200 µg/ml
RMB2377.00

IL-6 抗体 (C12-1-hIL-6) FITC

sc-32296 FITC
200 µg/ml
RMB2482.00

IL-6 抗体 (C12-1-hIL-6) PE

sc-32296 PE
200 µg/ml
RMB2580.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 488

sc-32296 AF488
200 µg/ml
RMB2685.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 546

sc-32296 AF546
200 µg/ml
RMB2685.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 594

sc-32296 AF594
200 µg/ml
RMB2685.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 647

sc-32296 AF647
200 µg/ml
RMB2685.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 680

sc-32296 AF680
200 µg/ml
RMB2685.00

IL-6 抗体 (C12-1-hIL-6) Alexa Fluor® 790

sc-32296 AF790
200 µg/ml
RMB2685.00

is it suitable for IhC (P) ,how many is the dilution?

Asked by: lilis
Thank you for your question. We warranty sc-32296 IL-6 (C12-1-hIL-6) antibody for IHC. We recommend a 1:50-1:500. dilution and here is our protocol : https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: BlakeJ
Date published: 2022-11-14

Are there any issues with using sc-32296: IL-6 (C12-1-hIL-6) mouse monoclonal primary antibody on mouse samples?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-32296 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Buon funzionamentoHo provato l'anticorpo qualche giorno fa ed ha dato una buona risposta in termini sia di specificità che di sensibilità.
Date published: 2022-04-23
Rated 4 out of 5 by from 功能良好之前申请了试用装，利用WB检测了小鼠脑组织样品，得到的结果还不错，可能是组织本身表达量不高，需要的上样量需提高。总的来说，值得购买。
Date published: 2018-05-12
Rated 4 out of 5 by from Works well for WBThis sample size trial Ab works on mouse lung lysates at a diln. of 1:200 (protein loaded (30ug)
Date published: 2018-03-15
Rated 1 out of 5 by from Not valid for IF on rat frozen liver sectionsRat liver frozen sections were subjected to immunofluorescence using IL-6 (C12-1-hIL-6) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. However, a positive signal was not clear on each slide.
Date published: 2018-03-14
Rated 2 out of 5 by from Used for skeletal muscleWe received a sample vial to try and complement our mRNA experiments, but the most prominent band detected was higher than the expected molecular weight.
Date published: 2017-12-20
Rated 5 out of 5 by from Great antibody for IF for rat brain4% PFA fixed rat brain section, 1:100 dilution, got very good result
Date published: 2017-10-24
Rated 4 out of 5 by from sc-32296Used on mouse brain samples in Western blot. Showed a sharp band at the correct size. Another band at around 40KD.
Date published: 2017-10-03
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
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IL-6 抗体 (C12-1-hIL-6) is rated 3.6 out of 5 by 10.
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