Date published: 2025-9-7

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IL-2 抗体 (F-5): sc-133118

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说明书
  • IL-2抗体(F-5)是小鼠单克隆IgG1 λ IL-2抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫human物种的IL-2的氨基酸21-153
  • IL-2 抗体 (F-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 IL-2
  • 抗IL-2抗体(F-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-2 (F-5): sc-133118免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是IL-2 Antibody (F-5) for WB applications. 的首选辅助检测试剂,这些试剂现与IL-2 Antibody (F-5) 打包提供(请参阅下面的订购信息)。

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関連項目

IL-2抗体(F-5)是一种IgG1 λ小鼠单克隆IL-2抗体(也称为IL2抗体、TCGF抗体、T细胞生长因子抗体、白细胞介素-2抗体、Aldesleukin抗体、参与T细胞克隆扩增调节的抗体、T细胞生长因子抗体或淋巴因子2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的IL-2蛋白。IL-2抗体(F-5)既可作为非偶联抗IL-2抗体形式提供,也可作为多种偶联形式的抗IL-2抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。淋巴因子是一组参与免疫系统细胞间通讯的信号分子。由活化的淋巴细胞分泌的淋巴因子包括蛋白质,如IL-2。这种蛋白质主要由通过T细胞受体复合物或其他有丝分裂原活化的辅助T细胞分泌。IL-2的靶细胞包括活化的辅助T细胞和细胞毒性T细胞,诱导它们增殖。IL-2的分泌也可以作为B细胞的生长因子。迄今为止,已经确定了三种不同的IL-2依赖性信号转导途径:由Src家族蛋白酪氨酸激酶介导的c-Fos/c-Jun诱导途径;c-Myc诱导途径;和雷帕霉素敏感途径;所有这些都会导致Bcl-2的诱导。此外,转录因子NFAT已被证明在调节IL-2转录中起主要作用,并与IL-2表达的年龄相关性下降相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-2 抗体 (F-5) 参考文献:

  1. STAT1、STAT3 和 STAT5 蛋白与 IL-2 受体的结合涉及 IL-2 受体 beta 链的不同亚域。  |  Delespine-Carmagnat, M., et al. 2000. Eur J Immunol. 30: 59-68. PMID: 10602027
  2. 利用 IL-2 提高抗肿瘤活性的策略:临床前模型以及对接受 IL-2 治疗的患者淋巴细胞抗肿瘤活性的分析。  |  Albertini, MR., et al. 1992. Biotherapy. 4: 189-98. PMID: 1599802
  3. 可溶性 IL-2 受体的生物学意义。  |  Caruso, C., et al. 1993. Mediators Inflamm. 2: 3-21. PMID: 18475497
  4. 埃斯库莱廷可抑制 T 细胞活化,但不会抑制 IL-2 的产生或 IL-2 受体的表达。  |  Kabelitz, D. and Al-Gorany, KH. 1989. Immunol Lett. 20: 241-5. PMID: 2654016
  5. 使用 IL-2 和 IL-2 产生的淋巴因子活化杀伤细胞治疗癌症患者的嗜酸性粒细胞活化。  |  Silberstein, DS., et al. 1989. J Immunol. 142: 2162-7. PMID: 2784151
  6. 白细胞介素 2(IL-2)被高亲和力的 IL-2 受体内化是 IL-2 依赖性 T 细胞系生长所必需的。  |  Kumar, A., et al. 1987. J Immunol. 139: 3680-4. PMID: 3119713
  7. 人 IL-2 单克隆抗体的结合特性及其在 IL-2 测量中的应用。  |  Brandt, E., et al. 1986. Lymphokine Res. 5 Suppl 1: S35-42. PMID: 3491262
  8. IL-2-Pseudomonas exotoxin 40(IL-2-PE40)对细胞介导免疫反应和体液免疫反应的双重作用机制。  |  Volk, HD., et al. 1994. J Immunol. 153: 2497-505. PMID: 8077661
  9. 用突变的 IL-2 类似物检测 IL-2 受体α亚基对高亲和性 IL-2 受体组装和功能的意外影响。  |  Kuziel, WA., et al. 1993. J Immunol. 150: 3357-65. PMID: 8468475
  10. 膀胱癌的局部 IL-2 免疫疗法。  |  Santoni, A., et al. 1997. Immunopharmacol Immunotoxicol. 19: 1-13. PMID: 9049656

订购信息

产品名称产品编号规格价格数量收藏夹

IL-2 抗体 (F-5)

sc-133118
200 µg/ml
RMB2377.00

IL-2 (F-5): m-IgG Fc BP-HRP 套装

sc-527093
200 µg Ab; 10 µg BP
RMB2662.00

IL-2 (F-5): m-IgG1 BP-HRP 套装

sc-532466
200 µg Ab; 20 µg BP
RMB2662.00

IL-2 抗体 (F-5) AC

sc-133118 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-2 抗体 (F-5) HRP

sc-133118 HRP
200 µg/ml
RMB2377.00

IL-2 抗体 (F-5) FITC

sc-133118 FITC
200 µg/ml
RMB2482.00

IL-2 抗体 (F-5) PE

sc-133118 PE
200 µg/ml
RMB2580.00

IL-2 抗体 (F-5) Alexa Fluor® 488

sc-133118 AF488
200 µg/ml
RMB2685.00

IL-2 抗体 (F-5) Alexa Fluor® 546

sc-133118 AF546
200 µg/ml
RMB2685.00

IL-2 抗体 (F-5) Alexa Fluor® 594

sc-133118 AF594
200 µg/ml
RMB2685.00

IL-2 抗体 (F-5) Alexa Fluor® 647

sc-133118 AF647
200 µg/ml
RMB2685.00

IL-2 抗体 (F-5) Alexa Fluor® 680

sc-133118 AF680
200 µg/ml
RMB2685.00

IL-2 抗体 (F-5) Alexa Fluor® 790

sc-133118 AF790
200 µg/ml
RMB2685.00

hi. I am attempting a western blot using the sc-133118 IL-2 antibody on mouse liver. However, the results show a 20 kD band. So, I have question of this result. Is this 20kD band right IL-2?

Asked by: SY P
Thank you for your question. Before we received your email and we have replied to your email. If you still have questions, please continue to contact us through previous email or contact our distributors in Korea. https://www.scbt.com/resources/distributors KOREA For ordering or product information contact us at: Essence Medical Inc. 417 Leaders B/D, Yatapdong 342-1 Bundanggu Seongnam Gyeonggi-do Korea Phone: 82-31-707-1544 Fax: 82-31-707-1999 Email: [email protected] Website: www.essencemedical.com Koram Biotech Corporation 6F, Koram Venture Building 907-1 Daechi-Dong Kang Nam-Ku Seoul 135-080 Korea Phone: 82 (2) 556-0311 Fax: 82 (2) 556-0828 Email: [email protected] Website: www.korambiotech.com
Answered by: Ling Chen
Date published: 2023-03-27

sorry. 25 kD is correct, not 20 kD. hi. I am attempting a western blot using the sc-133118 IL-2 antibody on mouse liver. However, the results show a 25 kD band. So, I have question of this result. Is this 25kD band right IL-2?

Asked by: SY P
Thank you for your question. Before I have replied to your email. If you still have questions, please contact our distributors in Korea. https://www.scbt.com/resources/distributors KOREA For ordering or product information contact us at: Essence Medical Inc. 417 Leaders B/D, Yatapdong 342-1 Bundanggu Seongnam Gyeonggi-do Korea Phone: 82-31-707-1544 Fax: 82-31-707-1999 Email: [email protected] Website: www.essencemedical.com Koram Biotech Corporation 6F, Koram Venture Building 907-1 Daechi-Dong Kang Nam-Ku Seoul 135-080 Korea Phone: 82 (2) 556-0311 Fax: 82 (2) 556-0828 Email: [email protected] Website: www.korambiotech.com
Answered by: Ling Chen
Date published: 2023-03-27

What is the recommended starting dilution for Western blot for IL-2 (F-5): sc-133118 monoclonal antibody?

Asked by: Trav11
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-133118.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_9, d_7, h_4CST
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Rated 4 out of 5 by from Good to haveThis antibody detects a good amount of recombinant IL-2 (10 ng/Lane) with a clean background. It can be used to detect purified IL-2 as well as IL-2-spiked human cell lysate by western blotting. I am working on conditions in order to detect endogenous IL-2 in either treated-cell lysate or conditioned-medium.
Date published: 2016-12-12
Rated 5 out of 5 by from Satisfactory Western Blot data of human recombinantSatisfactory Western Blot data of human recombinant IL-2 under reducing conditions. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Nice Western Blot data of human recombinant ILNice Western Blot data of human recombinant IL-2. -SCBT QC
Date published: 2013-06-21
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IL-2 抗体 (F-5) is rated 4.7 out of 5 by 3.
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