Date published: 2025-9-7
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IL-1RI Antibody (H-8) - Western Blotting - Image 300160
IL-1RI 抗体 (H-8): sc-393998. RAW 264. 7 (A), CCRF-CEM (B) 和 HeLa (C) 全细胞裂解液中 IL-1RI 表达的 Western 印迹分析.
IL-1RI Antibody (H-8) - Western Blotting - Image 300160
IL-1RI 抗体 (H-8): sc-393998. RAW 264. 7 (A), CCRF-CEM (B) 和 HeLa (C) 全细胞裂解液中 IL-1RI 表达的 Western 印迹分析.
IL-1RI Antibody (H-8) - Western Blotting - Image 402236
IL-1RI 抗体 (H-8) Alexa Fluor® 488: sc-393998 AF488. CCRF-CEM (A), HeLa (B) 和 NIH/3T3 (C) 全细胞裂解液中 IL-1RI 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
IL-1RI Antibody (H-8) - Western Blotting - Image 354309
IL-1RI 抗体 (H-8): sc-393998. KARPAS-299 (A) 和 AMJ2-C8 (B) 全细胞裂解液中 IL-1RI 表达的 Western 印迹分析.
IL-1RI 抗体 (H-8): sc-393998. RAW 264. 7 (A), CCRF-CEM (B) 和 HeLa (C) 全细胞裂解液中 IL-1RI 表达的 Western 印迹分析.

IL-1RI 抗体 (H-8): sc-393998

4.8(5)
寫評論提問

说明书
  • IL-1RI 抗体 H-8 是小鼠单克隆 IgG1 κ，IL-1RI抗体, 在34篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的IL-1RI的C-terminus的氨基酸539-563之间
  • IL-1RI 抗体 (H-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 IL-1RI
  • 抗IL-1RI抗体（H-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-1RI (H-8): sc-393998的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是IL-1RI Antibody (H-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IL-1RI Antibody (H-8) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

附加IL-1R抗体，包括IL-1ra, IL-1RAcP, IL-1RI和IL-1RII

IL-1RI抗体（H-8）是一种IgG1 κ小鼠单克隆IL-1RI抗体（也称为IL1R1抗体、p80抗体、IL1R抗体、IL1RA抗体、CD121A抗体或白细胞介素1受体α抗体），可通过蛋白质印迹法（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人类来源的IL-1RI蛋白。IL-1RI抗体（H-8）既可以非偶联抗IL-1RI抗体形式提供，也可以多种偶联形式的抗IL-1RI抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。已描述了三种与IL-1Rs结构相关的配体，包括两种激动剂IL-1α和IL-1β，以及一种特异性受体拮抗剂IL-1Rα。在IL-1调节的活动中，包括发热、急性期反应、结缔组织降解和免疫刺激活动。IL-1Rα分子也特异性地与IL-1Rs结合，但无法启动细胞内反应。已鉴定出两种不同的IL-1Rs，每种都属于Ig超家族，并在多种细胞和组织中广泛表达。尽管许多细胞类型同时表达I型和II型受体，但没有证据表明这些受体构成单个复合物的亚基。II型受体具有一个较短的29个氨基酸的胞质结构域，似乎不足以进行信号传导，而事实上，有大量证据表明IL-1仅通过I型IL-1R进行信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-1RI 抗体 (H-8) 参考文献:

  1. 白细胞介素-1 系统：受体, 配体和信号。  |  Dower, SK., et al. 1992. Chem Immunol. 51: 33-64. PMID: 1533129
  2. 通过哺乳动物表达从 B 细胞中克隆出一种新型 IL-1 受体，它在许多细胞类型中都有表达。  |  McMahan, CJ., et al. 1991. EMBO J. 10: 2821-32. PMID: 1833184
  3. IL-1RI参与正常生长板发育和骨骼建模。  |  Simsa-Maziel, S., et al. 2013. Am J Physiol Endocrinol Metab. 305: E15-21. PMID: 23592480
  4. 克隆人类 T 细胞的白细胞介素 1 受体。  |  Sims, JE., et al. 1989. Proc Natl Acad Sci U S A. 86: 8946-50. PMID: 2530587
  5. MARCH3通过介导IL-1RI的K48连接的多聚泛素化修饰和降解，减轻IL-1β引发的炎症。  |  Lin, H., et al. 2018. Proc Natl Acad Sci U S A. 115: 12483-12488. PMID: 30442668
  6. IL-1β/COX-2通路中IL-1RI和EP₂受体的表达量较高，以及非甾体类药物的新替代物--奥斯托醇在抑制COX-2方面的作用。  |  Kordulewska, NK., et al. 2019. Int J Mol Sci. 20: PMID: 30620999
  7. IL-4/IFN-γ炎症细胞因子谱导致鼻粘膜中IL-1β/IL-1RI/EP2/COX-2通路的调节不足。  |  Machado-Carvalho, L., et al. 2019. Respir Med. 150: 136-140. PMID: 30961940
  8. GAG定位在IL-1RI上；一种由糖基化双重效应调节的机制。  |  Azimzadeh Irani, M. and Ejtehadi, MR. 2019. Glycobiology. 29: 803-812. PMID: 31317192
  9. 可溶性 IL-1 受体与 IL-1 alpha, IL-1 beta 和 IL-1 受体拮抗剂的结合以及滑液中可溶性 IL-1 受体的水平。  |  Arend, WP., et al. 1994. J Immunol. 153: 4766-74. PMID: 7963543
  10. 败血症中脱落的 II 型 IL-1 受体水平升高。II型受体在调节IL-1反应中的潜在作用。  |  Giri, JG., et al. 1994. J Immunol. 153: 5802-9. PMID: 7989776
  11. 白细胞介素 1 只通过 I 型受体发出信号。  |  Sims, JE., et al. 1993. Proc Natl Acad Sci U S A. 90: 6155-9. PMID: 8327496
  12. 白细胞介素-1α和白细胞介素-1β与 I 型和 II 型白细胞介素-1 受体的独立结合。  |  Slack, J., et al. 1993. J Biol Chem. 268: 2513-24. PMID: 8428929

订购信息

产品名称产品编号规格价格数量收藏夹

IL-1RI 抗体 (H-8)

sc-393998
200 µg/ml
$316.00

IL-1RI (H-8): m-IgG Fc BP-HRP 套装

sc-530654
200 µg Ab; 10 µg BP
$354.00

IL-1RI (H-8): m-IgGκ BP-HRP 套装

sc-524173
200 µg Ab, 40 µg BP
$354.00

IL-1RI (H-8): m-IgG1 BP-HRP 套装

sc-544183
200 µg Ab; 20 µg BP
$354.00

IL-1RI 抗体 (H-8) AC

sc-393998 AC
500 µg/ml, 25% agarose
$416.00

IL-1RI 抗体 (H-8) HRP

sc-393998 HRP
200 µg/ml
$316.00

IL-1RI 抗体 (H-8) FITC

sc-393998 FITC
200 µg/ml
$330.00

IL-1RI 抗体 (H-8) PE

sc-393998 PE
200 µg/ml
$343.00

IL-1RI 抗体 (H-8) Alexa Fluor® 488

sc-393998 AF488
200 µg/ml
$357.00

IL-1RI 抗体 (H-8) Alexa Fluor® 546

sc-393998 AF546
200 µg/ml
$357.00

IL-1RI 抗体 (H-8) Alexa Fluor® 594

sc-393998 AF594
200 µg/ml
$357.00

IL-1RI 抗体 (H-8) Alexa Fluor® 647

sc-393998 AF647
200 µg/ml
$357.00

IL-1RI 抗体 (H-8) Alexa Fluor® 680

sc-393998 AF680
200 µg/ml
$357.00

IL-1RI 抗体 (H-8) Alexa Fluor® 790

sc-393998 AF790
200 µg/ml
$357.00

IL-1RI (H-8) 中和勝肽

sc-393998 P
100 µg/0.5 ml
$68.00

Is this product usable for IF with paraffin section?

Asked by: Raiogu
Thank you for your question. We do not recommend using this particular antibody for histological stainings. Please refer to Anti-IL-1RI Antibody (102): sc-66054 instead, which is recommended for this application.
Answered by: Tech Support Europe
Date published: 2022-10-06

Can we use this for FACS+

Asked by: Reddy
Thank you for your question. Anti-IL-1RI Antibody (H-8) has not been tested in flow cytometry. It is recommended for detection of IL-1RI of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Technical Support Europe
Date published: 2020-10-08

Can IL-1RI Antibody (H-8) FITC (sc-393998 FITC) be used with human cells (anti-human IL-1R1) for Flow cytometery?

Asked by: Biye
Thank you for your question. Anti-IL-1RI Antibody (H-8) is recommended for detection of IL-1RI of mouse, rat and human origin by WB, IP, IF and ELISA and is available conjugated to FITC for IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2020-06-15

IL-1RI Antibody (H-8): sc-393998 Is the uncojugated antibody is preserved in Na Azide?

Asked by: Isaac H
Thank you for your question. Yes, this antibody, IL-1RI Antibody (H-8): sc-393998, is provided in PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Tech Service
Date published: 2019-09-27

sc-393998 ab 로 paraffin section tissue staining IHC도 가능한가요

Asked by: coy151515
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다. sc-393998 ab 는 IHC에 아니고 WB, IP, IF 및 ELISA에만 추천합니다. paraffin section tissue staining에 사용하지 않는 것이 좋습니다.다른 문제 있으시면 전화:86 21-60936350 이메일: [email protected] 또는 온라인 문의로 문의하시면 됩니다. Thank you for your question, sc-393998 ab is only recommended for WB, IP, IF 및 ELISA,not IHC,you’d better not use it in paraffin section tissue staining. If you have any other question ,please contact our Technical Service Department at email [email protected],call us directly at (86 21) 6093-6350, or contact us by live chat.
Answered by: Technical Support 8
Date published: 2018-12-21

Can this antibody be used for staining on mouse tissue?

Asked by: Anna99
Yes, this antibody is recommended for use with mouse. The best way to stain mouse tissue would be to use a directly conjugated primary. This antibody is available conjugated to HRP (sc-393998 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-393998 PE), fluorescein (sc-393998 FITC), Alexa Fluor® 488 (sc-393998 AF488) or Alexa Fluor® 647 (sc-393998 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Worked like a charm in IHCThis antibody worked very well in IHC using human tissues
Date published: 2025-06-06
Rated 4 out of 5 by from IL1R1 ABUSED 1:500 FOR WESTERN BLOT. SIGNAL IS VERY CLEAR. RACOMMENDED,
Date published: 2018-01-05
Rated 5 out of 5 by from cost-effectiveThe antibody was used to detect the expression of IL-1RI after spinal cord injury. We recommend that the antibody be diluted more than 1:800 or 1:1000, which is very cost-effective.
Date published: 2017-09-16
Rated 5 out of 5 by from Good signal for WBIL-1RI (H-8) works great for Western blot (WB). Excepted band size at 80 kDa.
Date published: 2016-12-20
Rated 5 out of 5 by from Very good Western blot data of ILVery good Western blot data of IL-1RI expression in RAW 264.7, CCRF-CEM and HeLa whole cell lysates. -SCBT QC
Date published: 2013-09-05
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IL-1RI 抗体 (H-8) is rated 4.8 out of 5 by 5.
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