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IL-1α Antibody (ALF-161) - Western Blotting - Image 2611
IL-1α (ALF-161): sc-12741. 小鼠重组 IL-1α 的 Western 印迹分析.
IL-1α Antibody (ALF-161) - Western Blotting - Image 2611
IL-1α (ALF-161): sc-12741. 小鼠重组 IL-1α 的 Western 印迹分析.
IL-1α (ALF-161): sc-12741. 小鼠重组 IL-1α 的 Western 印迹分析.

IL-1α 抗体 (ALF-161): sc-12741

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寫評論提問
说明书
  • IL-1α抗体（ALF-161）是一种亚美尼亚仓鼠单克隆 IgG IL-1α抗体，被引用于8篇文献，发布于200 µg/ml
  • 针对全长纯化的mouse来源的重组IL-1α
  • IL-1 alpha 抗体 (ALF-161) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 IL-1α
  • 抗IL-1 alpha抗体（ALF-161）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物的中和，sc-12741 L，200 µg/0.1 ml
  • 目前，我们还没有完成IL-1α 抗体 (ALF-161)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

额外IL-1抗体，包括IL-1 α, IL-1 β, IL-1F5和IL-1F9

IL-1α抗体（ALF-161）是一种仓鼠单克隆IgG抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的IL-1α。IL-1α抗体（ALF-161）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。IL-1α在免疫反应和炎症中起着关键作用，它作为关键介质，影响T和B淋巴细胞以及自然杀伤（NK）细胞的活性。IL-1α主要位于细胞质中，可在细胞激活时释放，这对于调节炎症反应至关重要。IL-1α在细胞质中的存在使其能够在炎症刺激期间快速释放，从而实现免疫细胞与周围组织之间的快速沟通。IL-1α参与多种生理过程，包括肾脏发育和软骨退化，并涉及多种病理状况，如类风湿性关节炎、炎症性肠病和动脉粥样硬化。尽管IL-1α和IL-1β的遗传起源不同，但它们与相同的受体结合，这凸显了IL-1α在协调免疫反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-1α 抗体 (ALF-161) 参考文献:

  1. 明矾疫苗接种后，IL-1α 和炎症小体依赖性 IL-1β 可促进中性粒细胞浸润。  |  Oleszycka, E., et al. 2016. FEBS J. 283: 9-24. PMID: 26536497
  2. 炎症体和 Alarmins： 肾脏疾病中的IL-1β和IL-1α。  |  Anders, HJ. 2016. J Am Soc Nephrol. 27: 2564-75. PMID: 27516236
  3. IL-1α在炎症性疾病和癌症中的功能和调节。  |  Malik, A. and Kanneganti, TD. 2018. Immunol Rev. 281: 124-137. PMID: 29247991
  4. IL-1α的加工、信号转导及其在癌症进展中的作用  |  Chiu, JW., et al. 2021. Cells. 10: PMID: 33430381
  5. 白细胞介素 1α：IL-1α在自身免疫性疾病和炎症性疾病的发病机制和治疗中的作用综述  |  Cavalli, G., et al. 2021. Autoimmun Rev. 20: 102763. PMID: 33482337
  6. 抑制ASCT2可诱导肝星状细胞衰老，并通过IL-1α/NF-κB反馈途径改变促炎分泌组，从而抑制肝纤维化。  |  Wang, F., et al. 2022. Acta Pharm Sin B. 12: 3618-3638. PMID: 36176909
  7. 人类 TH17 细胞在 NLRP3 炎症小体激活时与 gasdermin E 孔接触释放 IL-1α。  |  Chao, YY., et al. 2023. Nat Immunol. 24: 295-308. PMID: 36604548
  8. 泪液中的 IL-7、IL-1α 和 IL-1β 水平可区分 IgG4 相关疾病和 Sjögren's 综合征。  |  Martín-Nares, E., et al. 2023. Clin Rheumatol. 42: 1101-1105. PMID: 36627528
  9. 胆汁酸诱导 IL-1α 并驱动小鼠树突状细胞产生不依赖于 NLRP3 炎症体的 IL-1β  |  Oleszycka, E., et al. 2023. Front Immunol. 14: 1285357. PMID: 38090554
  10. 通过阻断 IL-1α 挽救急性髓性白血病化疗期间的心脏功能障碍。  |  Zhou, X., et al. 2024. Eur Heart J. 45: 2235-2250. PMID: 38607560

订购信息

产品名称产品编号规格价格数量收藏夹

IL-1α 抗体 (ALF-161)

sc-12741
200 µg/ml
CNY2422.00

IL-1α 抗体 (ALF-161) L

sc-12741 L
200 µg/0.1 ml
CNY2422.00

IL-1α 抗体 (ALF-161) AC

sc-12741 AC
500 µg/ml, 25% agarose
CNY3189.00

IL-1α 抗体 (ALF-161) HRP

sc-12741 HRP
200 µg/ml
CNY2422.00

IL-1α 抗体 (ALF-161) FITC

sc-12741 FITC
200 µg/ml
CNY2527.00

IL-1α 抗体 (ALF-161) PE

sc-12741 PE
200 µg/ml
CNY2625.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 488

sc-12741 AF488
200 µg/ml
CNY2738.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 546

sc-12741 AF546
200 µg/ml
CNY2738.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 594

sc-12741 AF594
200 µg/ml
CNY2738.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 647

sc-12741 AF647
200 µg/ml
CNY2738.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 680

sc-12741 AF680
200 µg/ml
CNY2738.00

IL-1α 抗体 (ALF-161) Alexa Fluor® 790

sc-12741 AF790
200 µg/ml
CNY2738.00

Can IL-1α (ALF-161): sc-12741 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1α (ALF-161): sc-12741 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by 31796433 from Mutant BRAF and MEK Inhibitors Regulate the TumorProtein lysates were prepared in Laemmli sample buffer, separated by SDS-PAGE, and proteins were transferred to polyvinylidene dif-luoride membranes. Immunoreactivity was detected using HRP-con-jugated secondary antibodies (CalBioTech) and chemiluminescence substrate (Thermo Fisher Scientific) on a Versadoc Imaging Sys-tem (Bio-Rad). Primary antibodies, all from Cell Signaling Technol-ogy unless otherwise stated, were as follows: anti-phospho-ERK1/2 (T202/Y204), anti-ERK2 (Santa Cruz Biotechnology), anti-GSDME (Abcam), anti–cleaved caspase-3, anti-BIM/BOD (Enzo Life Sci-ences), anti-IL1α (Santa Cruz Biotechnology), anti-HMGB1, and anti-GAPDH. Cell supernatants were harvested in the absence of FBS in culture medium to avoid distortion of SDS-PAGE. After centrifu-gation to remove cell debris, cell supernatants were concentrated 10×using Amicon Ultra 10K (Sigma-Aldrich). Concentrates were mixed with Laemmli sample buffer (Bio-Rad) and analyzed via Western blotting. For T-cell culture, concentrates were further washed twice with PBS and once with RPMI-1640 medium by spin, and sterilized by filtration through a 0.2-μm filter. Protein gel staining was per-formed using Coomassie Brilliant Blue R-250.
Date published: 2020-10-27
Rated 3 out of 5 by jiapangpang1993 from need more testsI can not get a clear band in a 1:1000 dilution,I will change its concentration to see whether it can work or not
Date published: 2017-03-02
Rated 5 out of 5 by vagabond123 from IHC-PWe have detected positive immunofluorescence signal in human IHC-P sclides
Date published: 2016-12-12
Rated 3 out of 5 by HF from Good specificity in WBGood specificity in WB. -SCBT QC
Date published: 2013-08-16
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IL-1α 抗体 (ALF-161) is rated 4.0 out of 5 by 4.
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