Date published: 2025-9-8

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IL-15 抗体 (E-4): sc-8437

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说明书
  • IL-15 抗体 E-4 是小鼠单克隆 IgG2a κ,IL-15抗体, 在10篇文献中引用,规格为200 µg/ml
  • 针对氨基酸49-162的抗原,代表成熟IL-15,来源于human
  • IL-15 抗体 (E-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 IL-15
  • 抗IL-15抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-15 (E-4): sc-8437免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 IL-15 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IL-15 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

IL-15抗体(E-4)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人源IL-15。IL-15抗体(E-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。白介素-15(IL-15)也称为IL-T,是一种重要的细胞因子,通过刺激T细胞和自然杀伤(NK)细胞的增殖,同时增强B细胞扩张和抗体产生,在免疫反应中发挥着重要作用。与主要由淋巴细胞产生的IL-2不同,IL-15由多种细胞分泌,包括巨噬细胞、上皮细胞、肌肉组织和胎盘,这凸显了其来源和功能的多样性。IL-15由162个氨基酸组成,经过裂解形成成熟形式,对于维持记忆性T细胞和激活淋巴因子激活的杀伤(LAK)细胞至关重要。IL-15已被证明可作为人类血液T淋巴细胞的趋化因子,进一步强调了其在协调免疫反应中的重要性。IL-15与IL-2受体的β信号亚基共享,有证据表明IL-15也利用常见的IL-2受体γ亚基,类似于其他关键细胞因子,如IL-2、IL-4和IL-7,从而强调了其在免疫信号通路中的整体作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IL-15 抗体 (E-4) 参考文献:

  1. IL-15、麸质蛋白和 HLA-DQ8 驱动乳糜泻的组织破坏。  |  Abadie, V., et al. 2020. Nature. 578: 600-604. PMID: 32051586
  2. 癌症联合免疫疗法中的 IL-15。  |  Waldmann, TA., et al. 2020. Front Immunol. 11: 868. PMID: 32508818
  3. 利用 IL-15 信号增强 NK 细胞介导的癌症免疫疗法。  |  Ma, S., et al. 2022. Trends Immunol. 43: 833-847. PMID: 36058806
  4. snoRNA-IL-15 在脂肪细胞脂肪分解和 NK 细胞年轻化过程中的分子机制。  |  Zhang, Y., et al. 2023. Cell Metab. 35: 1457-1473.e13. PMID: 37329887
  5. 用于治疗 B 细胞恶性肿瘤的具有靶细胞限制 IL-15 活性的免疫细胞因子。  |  Zekri, L., et al. 2024. Sci Transl Med. 16: eadh1988. PMID: 38446900
  6. 紫外线 B 辐射可上调人体皮肤中 IL-15 的表达。  |  Mohamadzadeh, M., et al. 1995. J Immunol. 155: 4492-6. PMID: 7594612
  7. IL-15 具有诱导 B 细胞增殖和分化的刺激活性。  |  Armitage, RJ., et al. 1995. J Immunol. 154: 483-90. PMID: 7814861
  8. 新型细胞因子 IL-15 对 IL-2 受体 beta 和 gamma 链的利用。  |  Giri, JG., et al. 1994. EMBO J. 13: 2822-30. PMID: 8026467
  9. 克隆出一种与白细胞介素-2 受体 beta 链相互作用的 T 细胞生长因子。  |  Grabstein, KH., et al. 1994. Science. 264: 965-8. PMID: 8178155
  10. 一种淋巴因子,暂定名为白细胞介素 T,由人类成人 T 细胞白血病系产生,可刺激 T 细胞增殖并诱导淋巴因子激活的杀伤细胞。  |  Burton, JD., et al. 1994. Proc Natl Acad Sci U S A. 91: 4935-9. PMID: 8197160
  11. 人类白细胞介素-15受体α链的功能特征以及IL15RA和IL2RA基因的密切联系。  |  Anderson, DM., et al. 1995. J Biol Chem. 270: 29862-9. PMID: 8530383
  12. 诱导和调节小鼠巨噬细胞中 IL-15 的表达。  |  Doherty, TM., et al. 1996. J Immunol. 156: 735-41. PMID: 8543827

订购信息

产品名称产品编号规格价格数量收藏夹

IL-15 抗体 (E-4)

sc-8437
200 µg/ml
RMB2377.00

IL-15 (E-4): m-IgG Fc BP-HRP 套装

sc-528234
200 µg Ab; 10 µg BP
RMB2662.00

IL-15 (E-4): m-IgGκ BP-HRP 套装

sc-520560
200 µg Ab, 40 µg BP
RMB2662.00

IL-15 (E-4): m-IgG2a BP-HRP 套装

sc-547053
200 µg Ab; 10 µg BP
RMB2662.00

IL-15 抗体 (E-4) AC

sc-8437 AC
500 µg/ml, 25% agarose
RMB3129.00

IL-15 抗体 (E-4) HRP

sc-8437 HRP
200 µg/ml
RMB2377.00

IL-15 抗体 (E-4) FITC

sc-8437 FITC
200 µg/ml
RMB2482.00

IL-15 抗体 (E-4) PE

sc-8437 PE
200 µg/ml
RMB2580.00

IL-15 抗体 (E-4) Alexa Fluor® 488

sc-8437 AF488
200 µg/ml
RMB2685.00

IL-15 抗体 (E-4) Alexa Fluor® 546

sc-8437 AF546
200 µg/ml
RMB2685.00

IL-15 抗体 (E-4) Alexa Fluor® 594

sc-8437 AF594
200 µg/ml
RMB2685.00

IL-15 抗体 (E-4) Alexa Fluor® 647

sc-8437 AF647
200 µg/ml
RMB2685.00

IL-15 抗体 (E-4) Alexa Fluor® 680

sc-8437 AF680
200 µg/ml
RMB2685.00

IL-15 抗体 (E-4) Alexa Fluor® 790

sc-8437 AF790
200 µg/ml
RMB2685.00

Could this antibody stain tissues from mice?

Asked by: qihang
Thank you for your question. IL-15 Antibody (E-4) is only recommended for detection of IL-15 of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our tests with this antibody we cannot recommend it for use in cells or tissues from mice.
Answered by: Tech Support Europe
Date published: 2021-08-16

I am using IL-15 (E-4): sc-8437 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Buon funzionamento, moderato background in IHCL'anticorpo è stato testato su mammella ed intestino. La reattività specifica è buona ma occasionalmente si osserva staining aspecifico. Da interpretare con attenzione.
Date published: 2024-03-19
Rated 4 out of 5 by from I'm happy to use this antibody.I bought this antibody about a month ago, I chose to dilute the 1:200 do Western blot. I got a clear WB band antibody. I'm so happy.
Date published: 2017-05-16
Rated 5 out of 5 by from Good effectWorks good in Western at 1:1000 dilution, the result is perfect.
Date published: 2017-04-28
Rated 5 out of 5 by from Western blotting was used to get the correct bandWestern blotting detection of human breast cancer cells, 1:200 dilution, get the right band.
Date published: 2017-02-07
Rated 5 out of 5 by from HIghly recommended monoclonal antibodyPerfect staining with IL-15 Antibody (E-4) by IHCP
Date published: 2017-01-21
Rated 5 out of 5 by from it is very good qualitificationit is very good qualitification, it can get us the good band
Date published: 2016-12-06
Rated 5 out of 5 by from Obvious effectWe would to use the IL-15 sc-8437 in Western blots. detection of human thymus, lymphoid organ,obvious effect,Molecular Weight : 15 kDa.
Date published: 2016-12-03
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with human hCMEC/D3 cells(human brain microvessel cell). -SCBT Publication Review
Date published: 2015-07-20
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IL-15 抗体 (E-4) is rated 4.7 out of 5 by 12.
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