Date published: 2025-9-6

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IGFBP1 抗体 (H-5): sc-55474

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说明书
  • IGFBP1 抗体 H-5 是小鼠单克隆 IgG1 κ,IGFBP1抗体, 在16篇文献中引用,规格为200 µg/ml
  • 免疫human物种的IGFBP1的氨基酸121-240
  • IGFBP1 抗体 (H-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 IGFBP1
  • 抗IGFBP1抗体(H-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IGFBP1 (H-5): sc-55474免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 IGFBP1 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IGFBP1 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

IGFBP1 抗体 (H-5) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过各种应用检测小鼠、大鼠和人样本中的 IGFBP1,包括免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA)。IGFBP1 (H-5) 抗体有非共轭型和多种共轭型,如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。胰岛素样生长因子结合蛋白(IGFBPs)是 IGF 信号系统的重要调节因子,IGFBP1 通过与 IGF 结合并影响其在细胞外环境中的分布,在调节 IGF 生物利用度方面发挥着重要作用。IGFBP1 的调控尤为重要,因为胰岛素的产生对 IGFBP1 有负向调控作用,从而使 IGFBP1 成为新陈代谢过程和胎儿发育过程中的关键角色。在胎儿肝脏发育过程中,IGFBP1 基因表达明显偏高,并且会对营养变化和糖尿病状况做出反应,这凸显了 IGFBP1 在生长调节和代谢健康方面的重要性。IGFBP1 与其他 IGFBPs 相互作用,如 IGFBP2(可能充当 IGFs 的伴侣)和 IGFBP3(最丰富的 IGFBP,与相当一部分血清 IGFs 复合)。了解 IGFBP1 的动态和相互作用对于阐明 IGFBP1 在生长调节中的作用和对各种疾病的潜在影响至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IGFBP1 抗体 (H-5) 参考文献:

  1. 患有 IDDM 的少女体内 GH-IGF-I 系统发生了巨大变化:IGFBP1 是否可用于反映总体葡萄糖调节?  |  Halldin, MU., et al. 2000. Pediatr Diabetes. 1: 121-30. PMID: 15016222
  2. 西格列汀改善血糖控制与皮质醇或肝胰岛素敏感性的替代指标IGFBP-1无关。  |  Arnetz, L., et al. 2015. Growth Horm IGF Res. 25: 298-303. PMID: 26283275
  3. 根据IGF-1和IGFBP-1评估,HFpEF和HFrEF表现出不同的表型。  |  Faxén, UL., et al. 2017. J Card Fail. 23: 293-303. PMID: 27327968
  4. 脂联素、IGFBP-1和-2是预测前驱糖尿病和2型糖尿病的独立预测因子。  |  Brismar, K., et al. 2022. Front Endocrinol (Lausanne). 13: 1092307. PMID: 36686443
  5. 胰岛素样生长因子结合蛋白作为葡萄糖调节剂。  |  Baxter, RC. 1995. Metabolism. 44: 12-7. PMID: 7476305
  6. IGFBP-1 基因的结构和定位及其在肝脏再生过程中的表达。  |  Lee, J., et al. 1994. Hepatology. 19: 656-65. PMID: 7509771
  7. 胰岛素样生长因子  |  Schmid, C. 1995. Cell Biol Int. 19: 445-57. PMID: 7640658
  8. 调节新陈代谢的 IGF 系统。  |  Binoux, M. 1995. Diabete Metab. 21: 330-7. PMID: 8586149
  9. 正常和增生性疤痕成纤维细胞 IGFBPs 的差异表达。  |  Hathaway, CL., et al. 1996. J Surg Res. 60: 156-62. PMID: 8592408
  10. 胰岛素样生长因子结合蛋白(IGFBPs)及其调控动态。  |  Kelley, KM., et al. 1996. Int J Biochem Cell Biol. 28: 619-37. PMID: 8673727
  11. 胰岛素样生长因子结合蛋白(IGFBP)-7 的合成与特性。重组人 mac25 蛋白可特异性结合 IGF-I 和 -II。  |  Oh, Y., et al. 1996. J Biol Chem. 271: 30322-5. PMID: 8939990
  12. 在 IUGR 胎盘的表达谱中,IGFBP1 和 Follistatin-like 3 基因明显上调。  |  Okamoto, A., et al. 2006. Placenta. 27: 317-21. PMID: 16338475

订购信息

产品名称产品编号规格价格数量收藏夹

IGFBP1 抗体 (H-5)

sc-55474
200 µg/ml
RMB2377.00

IGFBP1 (H-5): m-IgG Fc BP-HRP 套装

sc-528592
200 µg Ab; 10 µg BP
RMB2662.00

IGFBP1 (H-5): m-IgGκ BP-HRP 套装

sc-521006
200 µg Ab, 40 µg BP
RMB2662.00

IGFBP1 (H-5): m-IgG1 BP-HRP 套装

sc-543014
200 µg Ab; 20 µg BP
RMB2662.00

IGFBP1 抗体 (H-5) AC

sc-55474 AC
500 µg/ml, 25% agarose
RMB3129.00

IGFBP1 抗体 (H-5) HRP

sc-55474 HRP
200 µg/ml
RMB2377.00

IGFBP1 抗体 (H-5) FITC

sc-55474 FITC
200 µg/ml
RMB2482.00

IGFBP1 抗体 (H-5) PE

sc-55474 PE
200 µg/ml
RMB2580.00

IGFBP1 抗体 (H-5) Alexa Fluor® 488

sc-55474 AF488
200 µg/ml
RMB2685.00

IGFBP1 抗体 (H-5) Alexa Fluor® 546

sc-55474 AF546
200 µg/ml
RMB2685.00

IGFBP1 抗体 (H-5) Alexa Fluor® 594

sc-55474 AF594
200 µg/ml
RMB2685.00

IGFBP1 抗体 (H-5) Alexa Fluor® 647

sc-55474 AF647
200 µg/ml
RMB2685.00

IGFBP1 抗体 (H-5) Alexa Fluor® 680

sc-55474 AF680
200 µg/ml
RMB2685.00

IGFBP1 抗体 (H-5) Alexa Fluor® 790

sc-55474 AF790
200 µg/ml
RMB2685.00

For the example immunoperoxidase staining with IGFBP1 Antibody (H-5): sc-55474 antibody? At what concentration did you use for human placenta tissue?

Asked by: T_1234
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Can IGFBP1 (H-5): sc-55474 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, IGFBP1 (H-5): sc-55474 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-04-02
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Rated 5 out of 5 by from Good antibody!This primary antibody was used to examine the IGFBP1 in human endometrial cancer cell line HEC-1-B by western blotting, and the specific band was observed.
Date published: 2017-05-08
Rated 4 out of 5 by from WB with none spicific bandWe could see one dim 30kd band but with other none specific band by WB
Date published: 2016-12-30
Rated 5 out of 5 by from Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic stainingBeautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Strong band in WB in HepG2 whole cell lysateStrong band in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Robust signal in WB in human recombinant IGFBP1Robust signal in WB in human recombinant IGFBP1. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Good publishable data in WB in human HepG2Good publishable data in WB in human HepG2 cells at 1:500 dilution. -SCBT Publication Review
Date published: 2015-01-09
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IGFBP1 抗体 (H-5) is rated 4.9 out of 5 by 7.
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