Date published: 2025-9-6
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IGF-1 Receptor β/IGF1R Antibody (F-1) - Western Blotting - Image 352078
IGF-IRβ (F-1): sc-390130. A-431 (A), MCF7 (B), NIH/3T3 (C) 和 HeLa (D) 全细胞裂解液中 IGF-IRβ 表达的 Western 印迹分析.
IGF-IRβ (F-1): sc-390130. A-431 (A), MCF7 (B), NIH/3T3 (C) 和 HeLa (D) 全细胞裂解液中 IGF-IRβ 表达的 Western 印迹分析.

IGF-1 Receptor β/IGF1R 抗体 (F-1): sc-390130

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寫評論提問

说明书
  • IGF-1 Receptor β/IGF1R 抗体 F-1 是小鼠单克隆 IgG2a κ，IGF-1 Receptor β/IGF1R抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫human物种的IGF-IRβ的氨基酸741-800
  • IGF-1 Receptor β/IGF1R 抗体 (F-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 IGF-IRβ
  • 抗IGF-1 Receptor β/IGF1R抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IGF-1 Receptor β/IGF1R (F-1): sc-390130的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是IGF-1 Receptor β/IGF1R Antibody (F-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IGF-1 Receptor β/IGF1R Antibody (F-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加IGF Receptor抗体，包括IGF-IR, IGF-IR α, IGF-IR β和IGF-IIR

IGF-IRβ抗体（F-1）是一种IgG2a κ小鼠单克隆IGF-IR β抗体（也称为IGF1R抗体、CD221抗体或胰岛素样生长因子1受体抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的IGF-IR β蛋白。IGF-IRβ抗体（F-1）既可以是非偶联的抗IGF-IR β抗体形式，也可以是多种偶联形式的抗IGF-IR β抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。受体酪氨酸激酶（RTK）是跨膜分子支架，可影响细胞过程，包括细胞周期、细胞迁移、细胞代谢、细胞存活、增殖和分化。胰岛素样生长因子-I受体（IGF-IR）是一种RTK，可刺激多种不同细胞类型的生长，阻断细胞凋亡，作为多种生长激素反应的中间体，并可能刺激某些类型的癌症的生长。IGF-IR的同源配体胰岛素样生长因子-I（IGF-I）促进IGF-IR与Shc、GRB2和Sos1的结合，从而启动Ras和ERK激酶级联反应，从而改变转录因子活性，例如激活Elk转录因子。胰岛素受体底物（IRS）-1和-2的模块式磷酸酪氨酸结合（PTB）结构域可与活性IGF-IR结合，并启动磷脂酰肌醇3激酶依赖的下游信号。人类IGF-IR基因定位于染色体15q26.3，编码一个由1376个氨基酸组成的前体蛋白，该前体蛋白裂解为α和β亚基。人类IGF-IIR基因定位于染色体6q26，编码一个由2491个氨基酸组成的跨膜蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IGF-1 Receptor β/IGF1R 抗体 (F-1) 参考文献:

  1. Shc和胰岛素样生长因子1受体在介导雌激素受体α向质膜转位中的作用。  |  Song, RX., et al. 2004. Proc Natl Acad Sci U S A. 101: 2076-81. PMID: 14764897
  2. 将抑制胰岛素样生长因子受体-1 酪氨酸激酶活性作为多发性骨髓瘤, 其他血液系统恶性肿瘤和实体瘤的治疗策略。  |  Mitsiades, CS., et al. 2004. Cancer Cell. 5: 221-30. PMID: 15050914
  3. I 型胰岛素样生长因子受体 mRNA 的反义寡核苷酸对体内乳腺癌生长的抑制涉及 ErbBs, PI-3K/Akt 和 p42/p44 MAPK 信号通路的失活，但不涉及对孕酮受体活性的调节。  |  Salatino, M., et al. 2004. Oncogene. 23: 5161-74. PMID: 15122317
  4. IL-1beta 会损害胰岛素样生长因子 i 诱导的分化以及胰岛素样生长因子 i 受体在成肌细胞中的下游激活信号。  |  Broussard, SR., et al. 2004. J Immunol. 172: 7713-20. PMID: 15187154
  5. 胰岛素受体底物-1/SHP-2相互作用是血管平滑肌细胞中胰岛素样生长因子-I 信号转导的表型依赖性开关机制。  |  Hayashi, K., et al. 2004. J Biol Chem. 279: 40807-18. PMID: 15272025
  6. 原发性乳腺癌中胰岛素样生长因子 1 受体的表达：免疫组化分析。  |  Shimizu, C., et al. 2004. Hum Pathol. 35: 1537-42. PMID: 15619214
  7. 胰岛素和 IGF-I 受体的激活机制和信号转导特异性。受体二聚体交联, 钟形曲线以及持续信号转导与瞬时信号转导。  |  De Meyts, P., et al. 1995. Ann N Y Acad Sci. 766: 388-401. PMID: 7486684
  8. 胰岛素/IGF-1 受体跨膜信号的分子缺陷。  |  Frattali, AL. and Pessin, JE. 1993. Ann N Y Acad Sci. 687: 77-89. PMID: 8323192
  9. 通过胰岛素受体底物 1 发出胰岛素和 IGF-I 信号。  |  Keller, SR., et al. 1993. Mol Reprod Dev. 35: 346-51; discussion 351-2. PMID: 8398112

订购信息

产品名称产品编号规格价格数量收藏夹

IGF-1 Receptor β/IGF1R 抗体 (F-1)

sc-390130
200 µg/ml
RMB2377.00

IGF-1 Receptor β/IGF1R (F-1): m-IgG Fc BP-HRP 套装

sc-530074
200 µg Ab; 10 µg BP
RMB2662.00

IGF-1 Receptor β/IGF1R (F-1): m-IgGκ BP-HRP 套装

sc-523419
200 µg Ab, 40 µg BP
RMB2662.00

IGF-1 Receptor β/IGF1R (F-1): m-IgG2a BP-HRP 套装

sc-547426
200 µg Ab; 10 µg BP
RMB2662.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) AC

sc-390130 AC
500 µg/ml, 25% agarose
RMB3129.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) HRP

sc-390130 HRP
200 µg/ml
RMB2377.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) FITC

sc-390130 FITC
200 µg/ml
RMB2482.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) PE

sc-390130 PE
200 µg/ml
RMB2580.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 488

sc-390130 AF488
200 µg/ml
RMB2685.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 546

sc-390130 AF546
200 µg/ml
RMB2685.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 594

sc-390130 AF594
200 µg/ml
RMB2685.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 647

sc-390130 AF647
200 µg/ml
RMB2685.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 680

sc-390130 AF680
200 µg/ml
RMB2685.00

IGF-1 Receptor β/IGF1R 抗体 (F-1) Alexa Fluor® 790

sc-390130 AF790
200 µg/ml
RMB2685.00

Can IGF-IRβ (F-1): sc-390130 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IGF-IRβ (F-1): sc-390130 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from IGF-IRβ Antibody (F-1) on mouse brain homogenategood antibody. No aspecific bands were present. I have used on mouse brain homogenates (20 micgograms of total proteins; 12% PAGE).
Date published: 2020-01-16
Rated 1 out of 5 by from Failed to work in WBs of mouse insulinoma cellsUsed the antibody at recommended minimal dilution and did not detect bands on WB using this antibody.
Date published: 2018-04-10
Rated 4 out of 5 by from Works fineIt's always good that we can get free samples to try from santa cruz and buy the one works better. This antibody worked for IGFR in my pilot experiments
Date published: 2017-11-06
Rated 5 out of 5 by from Great antibodyI have been using this antibody for several years and I am very happy with it
Date published: 2017-05-08
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
Rated 5 out of 5 by from good western blolt dataVery good Western blot data of IGF-IRβ expression in Hela cell, a little background,I use the dilution 1:800.
Date published: 2017-01-30
Rated 5 out of 5 by from highly recommendedThis antibody works in many different human cell lines such as MCF-7, Melanoma Cells, A431, RAW and A549 cells, we often use laboratory, the quality is good it is highly recommended
Date published: 2016-12-17
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~97 (beta-chain) and 200 kDa precursor forms of the target protein in an A431/HepG2/MCF7 cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
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IGF-1 Receptor β/IGF1R 抗体 (F-1) is rated 4.3 out of 5 by 9.
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