Date published: 2025-9-6
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IGF-1 Receptor α/IGF1R Antibody (2C8) - Western Blotting - Image 392586
IGF-IRα 抗体 (2C8) HRP: sc-463 HRP. 对 HeLa (A), A-431 (B), MCF7 (C), SK-BR-3 (D), NCI-H929 (E) 和 COLO 320 (F) 全细胞裂解液中 IGF-IRα 的表达进行直接 Western 印迹分析.
IGF-IRα 抗体 (2C8) HRP: sc-463 HRP. 对 HeLa (A), A-431 (B), MCF7 (C), SK-BR-3 (D), NCI-H929 (E) 和 COLO 320 (F) 全细胞裂解液中 IGF-IRα 的表达进行直接 Western 印迹分析.

IGF-1 Receptor α/IGF1R 抗体 (2C8): sc-463

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寫評論提問

说明书
  • IGF-1 Receptor α/IGF1R抗体(2C8)是小鼠单克隆IgG1 κ, 在34篇文献中引用，规格为200 µg/ml
  • 针对human来源的胎盘中纯化的IGF-I受体
  • 抗-IGF-1 Receptor α/IGF1R 抗体 (2C8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 IGF-IRα and complex
  • 抗IGF-1 Receptor α/IGF1R抗体（2C8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IGF-1 Receptor α/IGF1R (2C8): sc-463的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 IGF-1 Receptor α/IGF1R 抗体 (2C8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IGF-1 Receptor α/IGF1R 抗体 (2C8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 IGF Receptor 抗体包括 IGF-IR, IGF-IR α, IGF-IR β 和 IGF-IIR

IGF-IRα抗体（2C8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的IGF-IRα。IGF-IRα单克隆抗体（2C8）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。胰岛素样生长因子-I受体（IGF-IR）作为受体酪氨酸激酶发挥着关键作用，影响细胞生长、存活和分化等重要过程。IGF-IRα位于细胞膜上，对于胰岛素样生长因子-I（IGF-I）的作用至关重要，IGF-I可促进细胞增殖并抑制细胞凋亡，因此IGF-IRα在生长激素信号传导通路中扮演着重要角色。IGF-IR激活后，Shc、GRB2和Sos 1等适配蛋白被招募，启动下游信号级联，激活Ras和ERK激酶，最终影响转录因子的活性。此外，胰岛素受体底物（IRS）-1和-2蛋白与活性IGF-IR相互作用，触发磷脂酰肌醇3激酶依赖性信号通路，该通路对于细胞对生长因子的反应至关重要。人类胰岛素样生长因子受体基因位于15q26.3号染色体上，编码一种由1376个氨基酸组成的前体蛋白，该蛋白被切割成α和β亚基，这凸显了胰岛素样生长因子受体在细胞信号传导和癌症生物学中的复杂性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IGF-1 Receptor α/IGF1R 抗体 (2C8) 参考文献:

  1. Shc和胰岛素样生长因子1受体在介导雌激素受体α向质膜转位中的作用。  |  Song, RX., et al. 2004. Proc Natl Acad Sci U S A. 101: 2076-81. PMID: 14764897
  2. 将抑制胰岛素样生长因子受体-1 酪氨酸激酶活性作为多发性骨髓瘤, 其他血液系统恶性肿瘤和实体瘤的治疗策略。  |  Mitsiades, CS., et al. 2004. Cancer Cell. 5: 221-30. PMID: 15050914
  3. I 型胰岛素样生长因子受体 mRNA 的反义寡核苷酸对体内乳腺癌生长的抑制涉及 ErbBs, PI-3K/Akt 和 p42/p44 MAPK 信号通路的失活，但不涉及对孕酮受体活性的调节。  |  Salatino, M., et al. 2004. Oncogene. 23: 5161-74. PMID: 15122317
  4. IL-1beta 会损害胰岛素样生长因子 i 诱导的分化以及胰岛素样生长因子 i 受体在成肌细胞中的下游激活信号。  |  Broussard, SR., et al. 2004. J Immunol. 172: 7713-20. PMID: 15187154
  5. 胰岛素受体底物-1/SHP-2相互作用是血管平滑肌细胞中胰岛素样生长因子-I 信号转导的表型依赖性开关机制。  |  Hayashi, K., et al. 2004. J Biol Chem. 279: 40807-18. PMID: 15272025
  6. 原发性乳腺癌中胰岛素样生长因子 1 受体的表达：免疫组化分析。  |  Shimizu, C., et al. 2004. Hum Pathol. 35: 1537-42. PMID: 15619214
  7. 胰岛素和 IGF-I 受体的激活机制和信号转导特异性。受体二聚体交联, 钟形曲线以及持续信号转导与瞬时信号转导。  |  De Meyts, P., et al. 1995. Ann N Y Acad Sci. 766: 388-401. PMID: 7486684
  8. 胰岛素/IGF-1 受体跨膜信号的分子缺陷。  |  Frattali, AL. and Pessin, JE. 1993. Ann N Y Acad Sci. 687: 77-89. PMID: 8323192
  9. 通过胰岛素受体底物 1 发出胰岛素和 IGF-I 信号。  |  Keller, SR., et al. 1993. Mol Reprod Dev. 35: 346-51; discussion 351-2. PMID: 8398112

订购信息

产品名称产品编号规格价格数量收藏夹

IGF-1 Receptor α/IGF1R 抗体 (2C8)

sc-463
200 µg/ml
RMB2377.00

IGF-1 Receptor α/IGF1R (2C8): m-IgG Fc BP-HRP 套装

sc-528114
200 µg Ab; 10 µg BP
RMB2662.00

IGF-1 Receptor α/IGF1R (2C8): m-IgGκ BP-HRP 套装

sc-520414
200 µg Ab, 40 µg BP
RMB2662.00

IGF-1 Receptor α/IGF1R (2C8): m-IgG1 BP-HRP 套装

sc-542768
200 µg Ab; 20 µg BP
RMB2662.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) AC

sc-463 AC
500 µg/ml, 25% agarose
RMB3129.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) HRP

sc-463 HRP
200 µg/ml
RMB2377.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) FITC

sc-463 FITC
200 µg/ml
RMB2482.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) PE

sc-463 PE
200 µg/ml
RMB2580.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 488

sc-463 AF488
200 µg/ml
RMB2685.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 546

sc-463 AF546
200 µg/ml
RMB2685.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 594

sc-463 AF594
200 µg/ml
RMB2685.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 647

sc-463 AF647
200 µg/ml
RMB2685.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 680

sc-463 AF680
200 µg/ml
RMB2685.00

IGF-1 Receptor α/IGF1R 抗体 (2C8) Alexa Fluor® 790

sc-463 AF790
200 µg/ml
RMB2685.00

I am using IGF-IRα (2C8): sc-463 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibodyI try the sample antibody, works very well for detect in western blot.
Date published: 2017-09-21
Rated 5 out of 5 by from Western blotWe use this antibody analysis of signal transduction and apoptosis related proteins IGF-IRa in hippocampus by Western blot. The results indicated the target protein expression in rat brain tissue were obviously decreased. The antibody worked very well. According to the recommended concentration,the signal was strong in the controled sample group.
Date published: 2017-09-10
Rated 5 out of 5 by from great antibody that was easy to useWe used this antibody in a western blot experiment and got the correct band at the right molecular weight. Helpful cited publications
Date published: 2017-01-19
Rated 5 out of 5 by from Good specificity in WB in human AsPCGood specificity in WB in human AsPC-1, BxPC-3, and PANC-1 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Good publishable data in IP and WB in humanGood publishable data in IP and WB in human NIH/IGF-1R cells - transfected. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of 5 by from Strong pull down in IP in human MCFStrong pull down in IP in human MCF-7 & MDA-MB-231 cell lines. -SCBT Publication Review
Date published: 2015-03-20
Rated 4 out of 5 by from Robust signal in HeLa and ARobust signal in HeLa and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-01-09
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IGF-1 Receptor α/IGF1R 抗体 (2C8) is rated 4.9 out of 5 by 8.
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