Date published: 2025-9-6
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IGF-I Antibody (H-9) - Western Blotting - Image 389704
IGF-I 抗体 (H-9): sc-518040. 人重组 IGF-I 融合蛋白的 Western 印迹分析.
IGF-I Antibody (H-9) - Western Blotting - Image 389704
IGF-I 抗体 (H-9): sc-518040. 人重组 IGF-I 融合蛋白的 Western 印迹分析.
IGF-I Antibody (H-9) - Western Blotting - Image 390996
IGF-I 抗体 (H-9): sc-518040. 小鼠重组 IGF-I 的 Western 印迹分析.
IGF-I 抗体 (H-9): sc-518040. 人重组 IGF-I 融合蛋白的 Western 印迹分析.

IGF-I 抗体 (H-9): sc-518040

5.0(2)
寫評論提問

说明书
  • IGF-I 抗体 H-9 是小鼠单克隆 IgG2a κ，IGF-I抗体, 在18篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的的内部区域内的氨基酸75-94之间
  • IGF-I 抗体 (H-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 IGF-I
  • 抗IGF-I抗体（H-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IGF-I (H-9): sc-518040的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是IGF-I Antibody (H-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IGF-I Antibody (H-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

IGF-I抗体（H-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的IGF-I。IGF-I (H-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。胰岛素样生长因子I（IGF-I）是一种重要的多肽，在细胞生长和发育中发挥着至关重要的作用，它以自身分泌和旁分泌的方式刺激血管平滑肌细胞增殖。IGF-I主要存在于血清中，由肝脏分泌，对生长激素刺激反应，对生长和代谢的全身效应至关重要。IGF-I参与调节肾功能、骨骼形成和免疫反应，在各种生理过程中发挥着重要作用。IGF-I活性由六个IGF结合蛋白（IGFBP1-6）调节，这些蛋白可以增强或抑制作用，从而微调生物效应。IGF-I和IGF-II均通过IGF-IR受体发出信号，该受体与胰岛素受体结构相似，由两个细胞外α链和两个β链组成，负责将信号传递到细胞中，在生长调节和代谢过程中发挥着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IGF-I 参考文献:

  1. IGF-I刺激人类神经母细胞的趋化作用。1型IGF受体、IGF结合蛋白、磷脂酰肌醇-3激酶通路和纤溶酶系统的参与。  |  Puglianiello, A., et al. 2000. J Endocrinol. 165: 123-31. PMID: 10750042
  2. IGF-I介导高磷酸盐浓度对成骨细胞增殖的刺激作用。  |  Kanatani, M., et al. 2002. J Cell Physiol. 190: 306-12. PMID: 11857446
  3. 胰岛素样生长因子 IGF-I 和 IGF-II 及其受体在 K562 细胞生长和巨核细胞分化过程中的表达。  |  Aro, AL., et al. 2002. Leuk Res. 26: 831-7. PMID: 12127559
  4. 人类单克隆抗体片段与胰岛素样生长因子I和II结合，亲和力为皮摩尔级。  |  Zhao, Q., et al. 2011. Mol Cancer Ther. 10: 1677-85. PMID: 21750218
  5. 定量分析IGF-1与BI 836845的结合，BI 836845是一种针对IGF-1和IGF-2的候选治疗性抗体，以及该抗体对IGF-1:IGFBP-3复合物体外和雄性C57BL/6小鼠体内作用的影响。  |  Mireuta, M., et al. 2014. Endocrinology. 155: 703-15. PMID: 24424057
  6. 肿瘤坏死因子对胰岛素样生长因子-I（IGF-I）和IGF结合蛋白的调节。  |  Fan, J., et al. 1995. Am J Physiol. 269: R1204-12. PMID: 7503312
  7. IGF-I介导高钙浓度对成骨细胞增殖的刺激作用。  |  Sugimoto, T., et al. 1994. Am J Physiol. 266: E709-16. PMID: 8203509
  8. 胰岛素样生长因子（IGF）-I和IGF结合蛋白-1的结合促进成纤维细胞内胶原凝胶收缩。  |  Lee, YR., et al. 1996. Endocrinology. 137: 5278-83. PMID: 8940346
  9. 通过抗IGF-I单克隆抗体对胰岛素样生长因子I（IGF-I）的功能表位进行图谱分析。  |  Mañes, S., et al. 1997. Endocrinology. 138: 905-15. PMID: 9048589
  10. 针对人类IGF-I的单克隆抗体的物种和配体特异性的分子基础。  |  Van Wyk, JJ. and Bruton, ET. 1997. Endocrinology. 138: 4521-2. PMID: 9322977

订购信息

产品名称产品编号规格价格数量收藏夹

IGF-I 抗体 (H-9)

sc-518040
200 µg/ml
RMB2377.00

IGF-I (H-9): m-IgG Fc BP-HRP 套装

sc-531600
200 µg Ab; 10 µg BP
RMB2662.00

IGF-I (H-9): m-IgGκ BP-HRP 套装

sc-525335
200 µg Ab, 40 µg BP
RMB2662.00

IGF-I (H-9): m-IgG2a BP-HRP 套装

sc-547827
200 µg Ab; 10 µg BP
RMB2662.00

IGF-I 抗体 (H-9) AC

sc-518040 AC
500 µg/ml, 25% agarose
RMB3129.00

IGF-I 抗体 (H-9) HRP

sc-518040 HRP
200 µg/ml
RMB2377.00

IGF-I 抗体 (H-9) FITC

sc-518040 FITC
200 µg/ml
RMB2482.00

IGF-I 抗体 (H-9) PE

sc-518040 PE
200 µg/ml
RMB2580.00

IGF-I 抗体 (H-9) Alexa Fluor® 488

sc-518040 AF488
200 µg/ml
RMB2685.00

IGF-I 抗体 (H-9) Alexa Fluor® 546

sc-518040 AF546
200 µg/ml
RMB2685.00

IGF-I 抗体 (H-9) Alexa Fluor® 594

sc-518040 AF594
200 µg/ml
RMB2685.00

IGF-I 抗体 (H-9) Alexa Fluor® 647

sc-518040 AF647
200 µg/ml
RMB2685.00

IGF-I 抗体 (H-9) Alexa Fluor® 680

sc-518040 AF680
200 µg/ml
RMB2685.00

IGF-I 抗体 (H-9) Alexa Fluor® 790

sc-518040 AF790
200 µg/ml
RMB2685.00

Dear Sir/Madam Can we use this antibody for detecting zebrafish IGF-1.

Asked by: Momon
Thank you for your question. We have not validated the antibody for zebrafish. The antibody is recommended for mouse,rat and human.
Answered by: BlakeJ
Date published: 2025-01-21

Could I possibly get a sample of your IGF1 Antibody (sc-518040) to test out! We are testing IGF-I from equine samples, so reactivity is very possible but not confirmed. I would be more than happy to send you pictures confirming the antibody's use for WBs!

Asked by: Jaiden
Thank you for your question. Please email technical service on how to receive a sample. Please email [email protected]
Answered by: BlakeJ
Date published: 2024-03-28

Hi, can we use this (sc-518040) for IF and WB to detect IGF-1 in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody? Couldn't find any myself. Thanks!

Asked by: Jenn12
Thank you for your question . We do not have any publications for rat hippocampus. Here is a publication for rat in western blot . https://pubmed.ncbi.nlm.nih.gov/31037585/
Answered by: BlakeJ
Date published: 2023-02-15

dose it work with cryosectioning slice when do immunofluresence? Many many thanks for you! btw, my brain sample didn't fixed

Asked by: Wz_HH
Thank you for your question. This antibody is suitable for immunofluorescence experiment of frozen sections,If you have any further questions, please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Jiajun Z
Date published: 2022-11-22
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Rated 5 out of 5 by from Antibody recognizes IGF-I from mouse originAntibody works very well with a recombinant protein representing the 70 amino acid mature, mouse IGF-I protein -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Work well!1:100 diluted for wb of rat heart protein,the band is strong and special
Date published: 2019-10-27
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IGF-I 抗体 (H-9) is rated 5.0 out of 5 by 2.
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