Date published: 2025-9-6

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HXK I 抗体 (G-1): sc-46695

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说明书
  • HXK I 抗体 G-1 是小鼠单克隆 IgG1 κ,HXK I抗体, 在27篇文献中引用,规格为200 µg/ml
  • 免疫human物种的HXK I的氨基酸316-410
  • HXK I 抗体 (G-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 HXK I
  • 抗HXK I抗体(G-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HXK I (G-1): sc-46695免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 HXK I 抗体 (G-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与HXK I 抗体 (G-1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

HXK I 抗体(G-1)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 HXK I。HXK I (G-1) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。HXK I在葡萄糖代谢中起着至关重要的作用,催化葡萄糖转化为葡萄糖-6-磷酸,这是糖酵解途径的第一步。这种酶促活性对于细胞能量产生和代谢调节至关重要,特别是在能量需求高的组织中,如大脑、心脏和肾脏,这些组织中HXK I的表达量最高。HXK I对葡萄糖代谢的调节对于维持葡萄糖稳态至关重要,功能失调会导致代谢紊乱,包括糖尿病。此外,HXK I是四种己糖激酶同工酶之一,每种同工酶具有不同的调节特性和组织分布,这凸显了HXK I在更广泛的葡萄糖代谢及其对健康和疾病的影响中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HXK I 抗体 (G-1) 参考文献:

  1. 人类葡萄糖激酶基因:与早发非胰岛素依赖型(2 型)糖尿病有关的两个错义突变的分离, 特征和鉴定。  |  Stoffel, M., et al. 1992. Proc Natl Acad Sci U S A. 89: 7698-702. PMID: 1502186
  2. 肿瘤细胞中的葡萄糖磷酸化。对编码线粒体结合型己糖激酶的全长 cDNA 进行克隆, 测序和活性过表达。  |  Arora, KK., et al. 1990. J Biol Chem. 265: 6481-8. PMID: 2318862
  3. 针对六磷酸酶 1 基因缺失/六磷酸酶 2 基因阳性癌症的肿瘤不可知治疗策略  |  Xu, S. and Herschman, HR. 2019. Cancer Res. 79: 5907-5914. PMID: 31434645
  4. Hexokinase 1 的细胞定位调节葡萄糖的代谢命运。  |  De Jesus, A., et al. 2022. Mol Cell. 82: 1261-1277.e9. PMID: 35305311
  5. 来自肝星状细胞源性细胞外囊泡的 HK1 会促进肝细胞癌的发展。  |  Chen, QT., et al. 2022. Nat Metab. 4: 1306-1321. PMID: 36192599
  6. 一种新的己糖激酶 1(HK1)变异体表现为布歇-诺伊豪瑟综合征。  |  Peretz, RH., et al. 2023. Am J Med Genet A. 191: 624-629. PMID: 36541585
  7. 靶向己糖激酶 1 可减轻 NLRP3 介导的根尖牙周炎炎症:实验室研究  |  Qian, Y., et al. 2023. Int Endod J. 56: 734-747. PMID: 36938709
  8. 导致非血红蛋白溶血性贫血的双叶己糖激酶 1 (HK1) 变体:以 HK1 启动子变异为重点的病例系列和文献综述。  |  Ukonmaanaho, EM., et al. 2024. Br J Haematol. 204: 2040-2048. PMID: 38415930
  9. Hexokinase 1 能在能量应激时形成调节线粒体裂变的环。  |  Pilic, J., et al. 2024. Mol Cell. 84: 2732-2746.e5. PMID: 38981483
  10. 大鼠体内多种形式的己糖激酶:组织分布, 年龄依赖性和特性。  |  Katzen, HM. and Schimke, RT. 1965. Proc Natl Acad Sci U S A. 54: 1218-25. PMID: 5219826
  11. 人类己糖激酶 II:序列和与其他己糖激酶的同源性。  |  Deeb, SS., et al. 1993. Biochem Biophys Res Commun. 197: 68-74. PMID: 8250948
  12. 以融合蛋白形式表达的人III型己糖激酶羧基域的纯化和特征。  |  Palma, F., et al. 1996. Mol Cell Biochem. 155: 23-9. PMID: 8717435

订购信息

产品名称产品编号规格价格数量收藏夹

HXK I 抗体 (G-1)

sc-46695
200 µg/ml
$316.00

HXK I (G-1): m-IgGκ BP-HRP 套装

sc-520895
200 µg Ab, 40 µg BP
$354.00

HXK I 抗体 (G-1) AC

sc-46695 AC
500 µg/ml, 25% agarose
$416.00

HXK I 抗体 (G-1) HRP

sc-46695 HRP
200 µg/ml
$316.00

HXK I 抗体 (G-1) FITC

sc-46695 FITC
200 µg/ml
$330.00

HXK I 抗体 (G-1) PE

sc-46695 PE
200 µg/ml
$343.00

HXK I 抗体 (G-1) Alexa Fluor® 488

sc-46695 AF488
200 µg/ml
$357.00

HXK I 抗体 (G-1) Alexa Fluor® 546

sc-46695 AF546
200 µg/ml
$357.00

HXK I 抗体 (G-1) Alexa Fluor® 594

sc-46695 AF594
200 µg/ml
$357.00

HXK I 抗体 (G-1) Alexa Fluor® 647

sc-46695 AF647
200 µg/ml
$357.00

HXK I 抗体 (G-1) Alexa Fluor® 680

sc-46695 AF680
200 µg/ml
$357.00

HXK I 抗体 (G-1) Alexa Fluor® 790

sc-46695 AF790
200 µg/ml
$357.00

I am using HXK I (G-1): sc-46695 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works on human tissue samplesWe tried it on various human tissue samples and got satisfactory WB results.
Date published: 2020-09-21
Rated 5 out of 5 by from HXK I (G-1)HXK I (G-1) worked for murine samples. Highly recommended
Date published: 2019-12-04
Rated 4 out of 5 by from 1:5000 dilution gave great resultThis antibody worked fine with my cells. I did not fiddle around and 1:5000 dilution, overnight incubation gave great results
Date published: 2018-03-20
Rated 5 out of 5 by from It does work!It can be used in both human and mouse cell lines. The diluted is 1:1000.
Date published: 2018-02-06
Rated 5 out of 5 by from Good antibodyThis one gives very strong band, I would recommend to use.
Date published: 2017-08-24
Rated 5 out of 5 by from WB publishable data with human COSWB publishable data with human COS-7 cells - SCBT Publication Review
Date published: 2015-05-07
Rated 4 out of 5 by from Strong bands seen in WB using humanStrong bands seen in WB using human, rat and mouse brain tissue extracts. -SCBT QC
Date published: 2015-03-05
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-02-28
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HXK I 抗体 (G-1) is rated 4.6 out of 5 by 9.
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