Date published: 2025-9-7
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HuR/ELAV1 Antibody (3A2) - Western Blotting - Image 391092
HuR 抗体 (3A2) Alexa Fluor® 488: sc-5261 AF488. Jurkat (A), MOLT-4 (B), Ramos (C) 和 K-562 (D) 全细胞裂解液中 HuR 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
HuR 抗体 (3A2) Alexa Fluor® 488: sc-5261 AF488. Jurkat (A), MOLT-4 (B), Ramos (C) 和 K-562 (D) 全细胞裂解液中 HuR 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

HuR/ELAV1 抗体 (3A2): sc-5261

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寫評論提問

说明书
  • HuR/ELAV1 抗体 3A2 是小鼠单克隆 IgG1 κ，HuR/ELAV1抗体, 在526篇文献中引用，规格为200 µg/ml
  • 针对全长HuR，human起源
  • HuR/ELAV1 抗体 (3A2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 Xenopus来源的HuR, HuB, HuC and HuD
  • 抗HuR/ELAV1抗体（3A2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HuR/ELAV1 (3A2): sc-5261的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HuR/ELAV1 抗体 (3A2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HuR/ELAV1 抗体 (3A2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Hu 抗体 包括 HuC, HuD和HuR

HuR抗体（3A2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HuR蛋白。HuR (3A2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。HuR蛋白（也称为ELAV样RNA结合蛋白1）通过与目标mRNA 3'-非翻译区中的AU富集元素结合，在基因表达的转录后调控中发挥着关键作用。这种相互作用可稳定mRNA转录本，使其免于快速降解，从而影响参与细胞关键过程（如生长、分化和对应激的反应）的基因的表达水平。HuR蛋白普遍表达，在多种肿瘤中通常过度表达，这凸显了其在癌症生物学中的重要性。HuR包含三个RNA识别结构域，它们对于结合活性至关重要，而核和细胞质之间的穿梭则由核出口信号调控，从而允许根据细胞信号调节mRNA的稳定性和翻译。这种动态调控凸显了HuR在维持细胞稳态中的重要性，以及作为癌症和其他疾病治疗靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HuR/ELAV1 抗体 (3A2) 参考文献:

  1. 哺乳动物ELAV样蛋白HuD诱导神经元分化需要细胞质定位。  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
  2. 基于TRIM21的生物PROTAC突出了HuR降解的治疗益处。  |  Wu, X. and Xu, L. 2022. Adv Drug Deliv Rev. 184: 114179. PMID: 35248670
  3. CHIP通过介导eIF2α的泛素化和肿瘤抑制剂RBM5的上调来抑制A549细胞的增殖和迁移。  |  Ma, Q., et al. 2023. Clin Transl Oncol. 25: 1999-2014. PMID: 36947360
  4. HuR与肿瘤耐药性之间的关系。  |  Finan, JM., et al. 2023. Cancer Res. 83: 3507-3516. PMID: 37683260
  5. eIF2α磷酸化可引发肌张力障碍样运动，伴有D2受体和胆碱能起源以及异常神经元连接。  |  Fletcher, A., et al. 2023. Nat Commun. 14: 7093. PMID: 37925433
  6. ELAV 类 RNA 结合蛋白在人类神经母细胞瘤中的不同活性。  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
  7. Elav 样蛋白与 GAP-43 mRNA 3'- 非翻译区的保守调控元件结合。  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
  8. 鉴定 HuR 蛋白与 AUUUA 介导的 mRNA 衰变有关。  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
  9. 神经内分泌基因 Hel-N1 和 HuD 在小细胞肺癌中的差异表达：HuD 在变异表型中下调的证据。  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
  10. 神经发生过程中 Hu RNA 结合蛋白的顺序表达和作用  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
  11. 富含 AU 元素的结合蛋白 HuR（一种 ELAV 蛋白）稳定 RNA。  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
  12. 神经元特异性 hel-N1 和 HuD 作为神经母细胞瘤的新型分子标记物：HuD 信使 RNA 水平与良好预后特征的相关性。  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

订购信息

产品名称产品编号规格价格数量收藏夹

HuR/ELAV1 抗体 (3A2)

sc-5261
200 µg/ml
RMB2377.00

HuR/ELAV1 (3A2): m-IgG Fc BP-HRP 套装

sc-528135
200 µg Ab; 10 µg BP
RMB2662.00

HuR/ELAV1 (3A2): m-IgGκ BP-HRP 套装

sc-520439
200 µg Ab, 40 µg BP
RMB2662.00

HuR/ELAV1 (3A2): m-IgG1 BP-HRP 套装

sc-542781
200 µg Ab; 20 µg BP
RMB2662.00

HuR/ELAV1 抗体 (3A2) AC

sc-5261 AC
500 µg/ml, 25% agarose
RMB3129.00

HuR/ELAV1 抗体 (3A2) HRP

sc-5261 HRP
200 µg/ml
RMB2377.00

HuR/ELAV1 抗体 (3A2) FITC

sc-5261 FITC
200 µg/ml
RMB2482.00

HuR/ELAV1 抗体 (3A2) PE

sc-5261 PE
200 µg/ml
RMB2580.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 488

sc-5261 AF488
200 µg/ml
RMB2685.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 546

sc-5261 AF546
200 µg/ml
RMB2685.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 594

sc-5261 AF594
200 µg/ml
RMB2685.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 647

sc-5261 AF647
200 µg/ml
RMB2685.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 680

sc-5261 AF680
200 µg/ml
RMB2685.00

HuR/ELAV1 抗体 (3A2) Alexa Fluor® 790

sc-5261 AF790
200 µg/ml
RMB2685.00

Have you identified the exact epitope the Hur (3A2) antibody binds to now?

Asked by: SarahYC
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

HELLO，I want to do RIP and WB experiment in mouse，which antibody should I choose？HuR Antibody (3A2) or HuR Antibody (3A2) AC.？

Asked by: ncestt
Thank you for your question. If you need to use the same antibody for both Western blotting and immunoprecipitation, then we would recommend purchasing the unconjugated primary antibody, sc-5261: HuR (3A2). The ‘AC’ represents the primary antibody directly conjugated to agarose beads, and is only suitable for immunoprecipitation experiments.
Answered by: Technical Support
Date published: 2019-07-13

Is this antibody works well for detecting use flow cytometry?

Asked by: Yongxia
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-17

I HAVE A QUESTION ABOUT THIS ANTIBODY. IT IS POSSIBLE TO REACT POSITIVELY AGAINST GST-FUSION PROTEINS?.

Asked by: FLOPITZOTSOA
Thank you for your question. This monoclonal antibody, HuR (3A2): sc-5261, is recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and xenopus origin. The antibody is not recommended for detection of GST. However, the antibody is suitable for detection of GST-HuR, HuB, HuC or HuD fusion proteins.
Answered by: Technical Support
Date published: 2017-11-15

Hello, for research only I need to know whether this antibody (HuR (3A2): sc-5261) binds C-terminal or N-terminal HuR?

Asked by: Theresa Liebe
Thank you for your question. HuR Antibody (3A2): sc-5261 is raised against full length recombinant HuR of human origin, and we have not determined the exact epitope recognized by this mouse monoclonal antibody. According to the literature (PMID: 10737787), “the 3A2 epitope is located in RRM 1 (RNA Recognition Motif 1) of HuR, which is highly conserved among all ELAV proteins [82% identity between HuR, HuD, and Hel-N1/N2, and 72% identity between HuR and HuC].”
Answered by: Technical Support
Date published: 2017-06-08

Does this antibody work well with CLIP?

Asked by: beachbum
Thank you for your question. We have not tested this antibody for CLIP, and are not aware of any publications citing use of the antibody for that application. HuR Antibody (3A2): sc-5261 is a mouse monoclonal that has been cited in over 25 publications for immunoprecipitation (IP). Based on the antibody's performance in that application and the similarity with CLIP, it may also be suitable for that application, but that would have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-03-10

What is the epitope of HuR Antibody (3A2): sc-5261?

Asked by: Dr Ninau Qelp
HuR Antibody (3A2): sc-5261 was raised against full length HuR of human origin. We have not mapped the epitope.
Answered by: Tech Service 2
Date published: 2016-12-15

Can HuR Antibody (3A2): sc-5261 be used for bovine samples?

Asked by: Dr Ninau Qelp
Yes. We have several publications that cite using HuR Antibody (3A2): sc-5261 in bovine samples. Please see PMID: 18206386, 20018854, and 22879735
Answered by: Tech Service 2
Date published: 2016-12-15
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Rated 5 out of 5 by from Detection of HuR that contains L-AzidohomoalanineAntibody sc-5261 was used successfully to detect HuR that was synthesized in the presence of L-Azidohomoalanine (AHA), an analog of L-methionine. Using click-chemistry, AHA-conaining HuR was biotinylated, affinity-purified with magnetic streptavidin-beads, and subjected to Western blot analysis.
Date published: 2025-08-16
Rated 5 out of 5 by from Good antibodyThe antibody worked perfectly as predicted. Recommended.
Date published: 2024-12-04
Rated 5 out of 5 by from 效果很好一直用santa的HuR货号为 SC-5261的抗体，无论是WB一抗,还是免疫共沉淀又或者是免疫荧光实验，效果都很好。值得购买。
Date published: 2024-06-11
Rated 5 out of 5 by from HuRdling SenescenceImmunohistochemical staining was performed using an anti-HuR antibody (sc-5261, Santa Cruz, TX, USA; 1:50). Western blotting was performed using the anti-HuR antibody (1:4000). IF (1:500) IH (1:50)
Date published: 2021-03-24
Rated 5 out of 5 by from 效果非常好我们一直在用这只抗体，可以做蛋白免疫印迹，蛋白免疫共沉淀，免疫荧光，特异性很高，效果非常好用，物有所值
Date published: 2020-08-17
Rated 1 out of 5 by from This review is for C23 Antibody (MS-3).C23 Antibody (MS-3) (Lot #H0818), which I purchased Nov. 2018, is not detecting any signal in BT20 breast and Hey ovarian cancer cells. I did IB multiple times with no success.
Date published: 2019-02-06
Rated 4 out of 5 by from review of sc-5261The anti-HuR 3A2 works very well in immunofluorescence
Date published: 2018-07-27
Rated 5 out of 5 by from HuR Antibody (3A2)HuR Antibody (3A2) works good in western blot from Cytosol fractions.
Date published: 2018-03-08
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HuR/ELAV1 抗体 (3A2) is rated 4.7 out of 5 by 28.
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