Date published: 2025-9-11
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HSV-1 gD Antibody (DL6) - Western Blotting - Image 6518
HSV gD (DL6): sc-21719. 对 HSV-1 感染的 Vero 全细胞裂解液中 HSV gD 的表达进行 Western 印迹分析.
HSV-1 gD Antibody (DL6) - Western Blotting - Image 6518
HSV gD (DL6): sc-21719. 对 HSV-1 感染的 Vero 全细胞裂解液中 HSV gD 的表达进行 Western 印迹分析.
HSV gD (DL6): sc-21719. 对 HSV-1 感染的 Vero 全细胞裂解液中 HSV gD 的表达进行 Western 印迹分析.

HSV-1 gD 抗体 (DL6): sc-21719

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寫評論提問

说明书
  • HSV-1 gD 抗体 DL6 是小鼠单克隆 IgG2a κ，HSV-1 gD抗体, 在75篇文献中引用，规格为200 µg/ml
  • 表位图谱 HSV gD的氨基酸272-279
  • HSV-1 gD 抗体 (DL6) 推荐用于WB, IP 和 FCM，检测HSV-1 来源的 HSV-1 glycoprotein D
  • 抗HSV-1 gD抗体（DL6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSV-1 gD (DL6): sc-21719的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是HSV-1 gD Antibody (DL6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HSV-1 gD Antibody (DL6) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外HSV Protein抗体，包括HSV-1 gD, HSV-1, HSV-1 Envelope Protein, HSV-1 gB, HSV-1 gC, HSV-1 gC Envelope Protein, HSV-1 gE, HSV-1 gE Envelope Protein, HSV-1 gG, HSV-1 gG Envelope Protein, HSV-1 Glycoprotein B, HSV-1 ICP0, HSV-1 ICP27, HSV-1 ICP35, HSV-1 ICP4和HSV-1 ICP4 Immediate Early Protein

HSV-1 gD 抗体（DL6）是一种鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和流式细胞术（FCM）检测源自单纯疱疹病毒 1 型（HSV-1）的 gD 蛋白。HSV-1 gD (DL6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。糖蛋白D（gD）在单纯疱疹病毒进入宿主细胞的过程中起着关键作用，因为gD对于病毒包膜与宿主细胞膜的融合至关重要。这一过程对于病毒感染和繁殖至关重要，因为糖蛋白D与目标细胞表面的特定受体相互作用，促进病毒进入。值得注意的是，糖蛋白D与细胞受体结合的能力对于病毒的感染性至关重要，因为gD会引发一系列构象变化，导致膜融合。糖蛋白D与gH/gL异二聚体的相互作用进一步增强了这一过程，凸显了gD在病毒生命周期中的重要性。了解糖蛋白D的功能对于制定针对单纯疱疹病毒感染的治疗策略至关重要，因为靶向gD可以抑制病毒的进入和传播。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSV-1 gD 抗体 (DL6) 参考文献:

  1. 适用于通过快速培养试验识别单纯疱疹病毒特异类型的单克隆抗体。  |  Bystrická, M., et al. 1999. Acta Virol. 43: 399-402. PMID: 10825932
  2. 转染细胞中由单纯疱疹病毒 2 糖蛋白 gB, gD, gH 和 gL 介导的细胞-细胞融合的特征。  |  Muggeridge, MI. 2000. J Gen Virol. 81: 2017-2027. PMID: 10900041
  3. 缺乏单个糖蛋白的 1 型单纯疱疹病毒颗粒中糖蛋白 B, C, D 和 H 的组装和组织：没有证据表明这些分子形成了复合体。  |  Rodger, G., et al. 2001. J Virol. 75: 710-6. PMID: 11134284
  4. 1型单纯疱疹病毒糖蛋白gB, gD, gH和gL诱导细胞融合的质膜要求。  |  Browne, H., et al. 2001. J Gen Virol. 82: 1419-1422. PMID: 11369886
  5. 针对 1 型和 2 型单纯疱疹病毒的常见类型和特异类型单克隆抗体。  |  Bystrická, M., et al. 1991. Acta Virol. 35: 152-64. PMID: 1681713
  6. 1 型单纯疱疹病毒 B 型糖蛋白在病毒进入和细胞融合中的作用。  |  Cai, WH., et al. 1988. J Virol. 62: 2596-604. PMID: 2839688
  7. 生殖器疱疹的血清流行病学与控制：单纯疱疹病毒特异性抗体的价值。  |  Slomka, MJ. 1996. Commun Dis Rep CDR Rev. 6: R41-5. PMID: 8820192
  8. 针对糖蛋白 C 和 B 上不同抗原位点的单克隆抗体及其在单纯疱疹病毒感染中的保护能力。  |  Bystrická, M., et al. 1997. Acta Virol. 41: 5-12. PMID: 9199708
  9. 在 Cos 细胞转染系统中，1 型单纯疱疹病毒的糖蛋白 gB, gD 和 gHgL 是介导膜融合的必要和充分条件。  |  Turner, A., et al. 1998. J Virol. 72: 873-5. PMID: 9420303

订购信息

产品名称产品编号规格价格数量收藏夹

HSV-1 gD 抗体 (DL6)

sc-21719
200 µg/ml
RMB2377.00

HSV-1 gD (DL6): m-IgG Fc BP-HRP 套装

sc-528362
200 µg Ab; 10 µg BP
RMB2662.00

HSV-1 gD (DL6): m-IgGκ BP-HRP 套装

sc-520723
200 µg Ab, 40 µg BP
RMB2662.00

HSV-1 gD (DL6): m-IgG2a BP-HRP 套装

sc-547077
200 µg Ab; 10 µg BP
RMB2662.00

HSV-1 gD 抗体 (DL6) AC

sc-21719 AC
500 µg/ml, 25% agarose
RMB3129.00

HSV-1 gD 抗体 (DL6) HRP

sc-21719 HRP
200 µg/ml
RMB2377.00

HSV-1 gD 抗体 (DL6) FITC

sc-21719 FITC
200 µg/ml
RMB2482.00

HSV-1 gD 抗体 (DL6) PE

sc-21719 PE
200 µg/ml
RMB2580.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 488

sc-21719 AF488
200 µg/ml
RMB2685.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 546

sc-21719 AF546
200 µg/ml
RMB2685.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 594

sc-21719 AF594
200 µg/ml
RMB2685.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 647

sc-21719 AF647
200 µg/ml
RMB2685.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 680

sc-21719 AF680
200 µg/ml
RMB2685.00

HSV-1 gD 抗体 (DL6) Alexa Fluor® 790

sc-21719 AF790
200 µg/ml
RMB2685.00

Can this antibody used for Immunofluorescence staining or ICC? What is the fixation and antigen retrieval condition? Thank you.

Asked by: risa
Thank you for your question. We have not yet tested this antibody, HSV-1 gD Antibody (DL6): sc-21719, by IF or ICC and so the antibody is not recommended for IF or ICC at this time. 感谢您的提问。我们还没有验证过HSV-1 gD Antibody (DL6): sc-21719在IHC和ICC中的应用，所以该产品暂时不质保IF和ICC应用。
Answered by: Yanru Tao
Date published: 2023-12-25

Hello, does this antibody react against HSV2 GD? Thank you

Asked by: giulia
Thank you for your question. HSV-1 gD Antibody (DL6): sc-21719 detects its target protein based on an epitope mapping to amino acids 272-279 of HSV gD. No data on the antibody's biological function is available, it has only been evaluated for detection purposes in WB, IP and FCM applications.
Answered by: Tech Support Europe
Date published: 2023-09-18

where is the country of origin for Anti-HSV-1gD Antibody(DL6) HRP product.

Asked by: Stew
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-02-08

Do you have an anti HSV-1 glycoprotein D antibody that binds in the region AA 1-269? Conjugated for Western blotting, monoclonal or polyclonal, either works. thanks

Asked by: Lkeller11
Thank you for your question. No, unfortunately we do not currently offer an antibody with that epitope mapping. Please check back later!
Answered by: Technical Support
Date published: 2018-12-11

Can this antibody be used to stain HSV-1 gD infected mouse samples without non-specific staining?

Asked by: AbPolly
Thank you for your question. To avoid non-specific staining with anti-mouse secondaries, we recommend using directly conjugated primaries. This HSV-1 gD antibody is available conjugated to HRP, FITC, PE, AF488 and AF647.
Answered by: Technical Support 7
Date published: 2017-01-18
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Rated 5 out of 5 by from It works wellWe are pleased to get statisfactory results in both WB and IF exeperiments with this antibody.
Date published: 2020-07-29
Rated 5 out of 5 by from This antibody works great!!I have used this antibody for IP and western blot. It works great!
Date published: 2018-09-24
Rated 4 out of 5 by from Great featureI bought it about a year ago and make an experiment with it recently.
Date published: 2017-10-16
Rated 4 out of 5 by from 仍然信任这个品牌The products was applied not mentioned in the instruction. Unfortunately, it is not available in our test.
Date published: 2017-10-12
Rated 4 out of 5 by from great!HeLa cellにmoi of 1, 2でHSV-1(HF株)を感染させたが、moi of 1では6時間から、moi of 2では感染直後からダブレットのバンドが確認できた。
Date published: 2017-10-05
Rated 5 out of 5 by from western blot resultThe HSV-1 gD antibody analyisised by Western blot, It detects distinct bands at 61kda. The result shows no background with 5% non-fat milk blocking at 4℃ overnight.
Date published: 2017-07-29
Rated 5 out of 5 by from single bandHela cell was blotted with this antibodies at concentration 1:500. The result shows single and specific band.
Date published: 2017-07-08
Rated 5 out of 5 by from Great Antibody for WBHSV-1 gD Antibody (DL6) works well, especially after overnight incubation. I used at 1:1000 dilution in 293T cell line
Date published: 2017-06-30
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HSV-1 gD 抗体 (DL6) is rated 4.8 out of 5 by 19.
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