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HSP 47 Antibody (E-4) - Western Blotting - Image 356342 — HSP 47 抗体 (E-4): sc-398579. 在 Sol8 (A), LADMAC (B), A-10 (C) 和 L6 (D) 全细胞裂解液中 HSP 47 表达的 Western 印迹分析.

HSP 47 抗体 (E-4): sc-398579. 在 Sol8 (A), LADMAC (B), A-10 (C) 和 L6 (D) 全细胞裂解液中 HSP 47 表达的 Western 印迹分析.

HSP 47 抗体 (E-4): sc-398579

Name: HSP 47抗体 (E-4)
Brand: SCBIO
SKU: sc-398579
Price: 2377.0 CNY
Availability: InStock

4.0(1)

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说明书

HSP 47抗体(E-4)是小鼠单克隆IgG₁ λ, 在1篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的HSP 47的N-terminus的氨基酸18-36之间
推荐用于 mouse, rat 和 human 来源的HSP 47 WB, IP, IF 和 ELISA检测
m-IgGλ BP-HRP（小鼠 IgGλ 结合蛋白-HRP）是HSP 47 Antibody (E-4) 适用于 WB 应用。的首选辅助检测试剂。该试剂现可与HSP 47 Antibody (E-4) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGλ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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HSP 47 抗体 (E-4) 参考文献:

Hsp47是一种新型的胶原蛋白结合蛋白抑制酶伴侣、自身抗原和治疗靶点。 | Sauk, JJ., et al. 2005. Front Biosci. 10: 107-18. PMID: 15574354
热休克蛋白47 kDa（HSP47/SERPINH1）的起源和分子进化分析。 | Kumar, A., et al. 2017. Sci Rep. 7: 10394. PMID: 28871169
HSP47的突变热点及其在癌症和骨骼疾病中的潜在作用。 | Parveen, A., et al. 2020. Genomics. 112: 552-566. PMID: 30986427
细胞特异性消融 Hsp47 确定了受伤心脏中的胶原蛋白生成细胞。 | Khalil, H., et al. 2019. JCI Insight. 4: e128722. PMID: 31393098
热休克蛋白 47（HSP47）与含盘状蛋白结构域的受体 2（DDR2）结合，并调节其蛋白质的稳定性。 | Chen, J., et al. 2019. J Biol Chem. 294: 16846-16854. PMID: 31570520
ER-高尔基体转运过程中原胶原和HSP47的分类机制。 | Omari, S., et al. 2020. Matrix Biol. 93: 79-94. PMID: 32562852
胶原蛋白亚家族中新的特定HSP47功能。 | Köhler, A., et al. 2020. FASEB J. 34: 12040-12052. PMID: 32716577
新冠病毒（COVID-19）的纤维化亚型在心脏肌成纤维细胞中表现出增强的局部ER依赖性HSP47+表达。 | Jacobs, ER., et al. 2023. J Mol Cell Cardiol. 185: 1-12. PMID: 37839656
含有hERG-1和Hsp47的小胞外囊泡水平作为心血管疾病的潜在生物标志物。 | Osorio, LA., et al. 2024. Int J Mol Sci. 25: PMID: 38732154
胶原特异性分子伴侣HSP47的调控和功能。 | Nagata, K. and Hosokawa, N. 1996. Cell Struct Funct. 21: 425-30. PMID: 9118251

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
HSP 47 抗体 (E-4)	sc-398579	200 µg/ml	RMB2377.00
HSP 47 (E-4): m-IgGλ BP-HRP 套装	sc-524400	200 µg Ab, 40 µg BP	RMB2662.00
HSP 47 (E-4) 中和勝肽	sc-398579 P	100 µg/0.5 ml	RMB511.00

(1) In the web page, you show the example of Western Blot using the heat shocked various cells. Can you tell how was "heat shock", temperature and hours? (2) In lane C, D, there are two bands, not only 47kDa. Could you explain what is another band?

Asked by: H Kadokawa

Thank you for your question. We heat shocked samples at 39ºC for 5 hours. According to online sources, HSP47 does have a signal peptide, which may be cleaved. In addition, the protein is further processed by post-translational modifications. For these reason, multiple bands may be observed for this target. If you have additional questions, please contact our Asia Technical Support team for further assistance. You may reach them by phone at (86 21) 6093-6350, by email at [email protected] or by live chat directly on our website, www.scbt.com. Alternatively, you can contact one of our authorized distributors in your region, which can be found in the following link: https://www.scbt.com/scbt/resources/distributors

Answered by: Blake J

Date published: 2021-07-12

What application is the blocking peptide sc-398579 P appropriate for?

Asked by: DefinitelyNotMatt

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-25

Hi  What's is the antibody light chain type? Thanks 

Asked by: Sarah1

Thank you for your question. HSP 47 Antibody (E-4) is a mouse monoclonal IgG1 with lambda light chain. This antibody (sc-398579) is a brand new product and this information will be updated on our website and datasheet shortly.

Answered by: Technical Support

Date published: 2017-01-05

Rated 4 out of 5 by HF from Good specificity and signal strength forGood specificity and signal strength for WB in L8 and NIH/3T3 whole cell lysates. -SCBT QC

Date published: 2015-04-06

HSP 47 抗体 (E-4) is rated 4.0 out of 5 by 1.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	SERPINH1	Entrez Gene ID: 871	11q13.5	XM_011545327, NM_001207014, NM_001235	UniProt ID: P50454	610504
Mouse	Serpinh1	Entrez Gene ID: 12406	7 E2	XM_006507278, XM_006507279, NM_001111043, NM_001111044, NM_001285776, NM_009825	UniProt ID: P19324	N/A

HSP 47 抗体 (E-4): sc-398579

HSP 47 抗体 (E-4) 参考文献:

優選的 HSP 47 抗體是 HSP 47 抗體 (E-1): sc-13150.

HSP 47 抗体 (E-4)

HSP 47 (E-4): m-IgGλ BP-HRP 套装

HSP 47 (E-4) 中和勝肽

(1) In the web page, you show the example of Western Blot using the heat shocked various cells. Can you tell how was &quot;heat shock&quot;, temperature and hours? (2) In lane C, D, there are two bands, not only 47kDa. Could you explain what is another band?

What application is the blocking peptide sc-398579 P appropriate for?

Hi&nbsp; What's is the antibody light chain type? Thanks&nbsp;

(1) In the web page, you show the example of Western Blot using the heat shocked various cells. Can you tell how was "heat shock", temperature and hours? (2) In lane C, D, there are two bands, not only 47kDa. Could you explain what is another band?

Hi What's is the antibody light chain type? Thanks