Date published: 2025-9-7
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HSP 27 Antibody (F-4) - Western Blotting - Image 384034
HSP 27 抗体 (F-4) HRP: sc-13132 HRP. 在 A-431 (A), MCF7 (B), A-10 (C), MDA-MB-231 (D) 和 HeLa (E) 全细胞裂解液以及大鼠心脏组织提取物 (F) 中直接进行 HSP 27 表达的 western 印迹分析.
HSP 27 抗体 (F-4) HRP: sc-13132 HRP. 在 A-431 (A), MCF7 (B), A-10 (C), MDA-MB-231 (D) 和 HeLa (E) 全细胞裂解液以及大鼠心脏组织提取物 (F) 中直接进行 HSP 27 表达的 western 印迹分析.

HSP 27 抗体 (F-4): sc-13132

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寫評論提問

说明书
  • HSP 27 抗体 F-4 是小鼠单克隆 IgG2a κ，HSP 27抗体, 在136篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HSP 27的氨基酸32-108
  • HSP 27 抗体 (F-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HSP 27
  • 抗HSP 27抗体（F-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSP 27 (F-4): sc-13132的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 HSP 27 抗体 (F-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HSP 27 抗体 (F-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HSP 抗体 包括 HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60和HSP 65

HSP 27 抗体（F-4）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 HSP 27（HSPB1）蛋白。HSP 27 (F-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。HSP 27在细胞应激反应中起着至关重要的作用，它充当分子伴侣，通过防止聚集和促进蛋白质正确折叠来帮助维持蛋白质的稳态。在应激条件下（如热休克或氧化应激），这一功能对于保护细胞免受损伤尤为重要，否则可能导致细胞死亡。HSP 27与信号通路中的各种蛋白质相互作用，包括与细胞凋亡和细胞存活相关的蛋白质，从而影响关键的细胞过程。激素和细胞因子对HSP 27的调节进一步强调了HSP 27在健康和疾病中的重要性，包括在癌症生物学中的潜在作用，特别是在乳腺癌中，其表达水平可能与肿瘤进展和患者预后相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 27 抗体 (F-4) 参考文献:

  1. HspB1（Hsp 27）在视网膜中的表达和神经保护作用。  |  O'Reilly, AM., et al. 2010. Mol Neurobiol. 42: 124-32. PMID: 20514530
  2. 葡萄膜黑色素瘤中的HSP-27蛋白表达：与预测存活率的相关性。  |  Jmor, F., et al. 2012. Acta Ophthalmol. 90: 534-9. PMID: 21114636
  3. Hsp-27水平与血栓负荷与ST段抬高型心肌梗死患者的临床结果相关。  |  Tian, M., et al. 2017. Oncotarget. 8: 73733-73744. PMID: 29088740
  4. 温度变化后大肠杆菌中单个多肽的瞬时合成率。  |  Lemaux, PG., et al. 1978. Cell. 13: 427-34. PMID: 350413
  5. HSP-27和HSP-70对分枝杆菌感染期间巨噬细胞的保护性防御反应具有负向调节作用。  |  Saraswati, SSK., et al. 2023. Microbes Infect. 25: 105126. PMID: 36931492
  6. 氨基酸类似物和热休克对鸡胚成纤维细胞基因表达的影响。  |  Kelley, PM. and Schlesinger, MJ. 1978. Cell. 15: 1277-86. PMID: 569556
  7. 细胞因子和生长因子诱导人类星形胶质细胞中的 HSP27 磷酸化。  |  Satoh, J. and Kim, SU. 1995. J Neuropathol Exp Neurol. 54: 504-12. PMID: 7541446
  8. 肿瘤坏死因子-α诱导人类 hsp27 的磷酸化, 细胞定位和寡聚化发生变化，而 hsp27 是一种应激蛋白，可使细胞对这种细胞因子产生抵抗力。  |  Mehlen, P., et al. 1995. J Cell Biochem. 58: 248-59. PMID: 7673331
  9. 伴侣素 ATP 酶循环的动力学：对促进蛋白质折叠的影响。  |  Todd, MJ., et al. 1994. Science. 265: 659-66. PMID: 7913555
  10. 白细胞介素-1激活了一种新型蛋白激酶级联，导致Hsp27磷酸化。  |  Freshney, NW., et al. 1994. Cell. 78: 1039-49. PMID: 7923354
  11. 热休克蛋白 27,000 (Hsp27) 的生物学和临床意义：综述。  |  Ciocca, DR., et al. 1993. J Natl Cancer Inst. 85: 1558-70. PMID: 8411230

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 27 抗体 (F-4)

sc-13132
200 µg/ml
RMB2377.00

HSP 27 (F-4): m-IgG Fc BP-HRP 套装

sc-528267
200 µg Ab; 10 µg BP
RMB2662.00

HSP 27 (F-4): m-IgGκ BP-HRP 套装

sc-520602
200 µg Ab, 40 µg BP
RMB2662.00

HSP 27 抗体 (F-4) AC

sc-13132 AC
500 µg/ml, 25% agarose
RMB3129.00

HSP 27 抗体 (F-4) HRP

sc-13132 HRP
200 µg/ml
RMB2377.00

HSP 27 抗体 (F-4) FITC

sc-13132 FITC
200 µg/ml
RMB2482.00

HSP 27 抗体 (F-4) PE

sc-13132 PE
200 µg/ml
RMB2580.00

HSP 27 抗体 (F-4) Alexa Fluor® 488

sc-13132 AF488
200 µg/ml
RMB2685.00

HSP 27 抗体 (F-4) Alexa Fluor® 546

sc-13132 AF546
200 µg/ml
RMB2685.00

HSP 27 抗体 (F-4) Alexa Fluor® 594

sc-13132 AF594
200 µg/ml
RMB2685.00

HSP 27 抗体 (F-4) Alexa Fluor® 647

sc-13132 AF647
200 µg/ml
RMB2685.00

HSP 27 抗体 (F-4) Alexa Fluor® 680

sc-13132 AF680
200 µg/ml
RMB2685.00

HSP 27 抗体 (F-4) Alexa Fluor® 790

sc-13132 AF790
200 µg/ml
RMB2685.00

Can the HSP27 antibody detect HSP27 in 293 cells by western blot?

Asked by: Anonymous
Thank you for your question. sc-74414 E4BP4 (F-1) is recommended for human samples .The antibody should work for 293 cells .
Answered by: BlakeJ
Date published: 2025-08-06

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2018-03-15
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Rated 5 out of 5 by from very good antibodymouse gut tissues, 1:1000 dilution, very strong and clear bands were explored.
Date published: 2024-01-10
Rated 5 out of 5 by from Specific detection by western blottingThis antibody is useful for detection of mouse HSP27 in mammary epithelial cells by western blotting.
Date published: 2021-11-15
Rated 2 out of 5 by from Funzionamento discretoAnticorpo testato. Riconosce la proteina over-espressa, meno bene quella endogena
Date published: 2020-07-09
Rated 4 out of 5 by from recommended for WBWorks very well for whole cell lysates of HEp-2 and RKO cells when the antibody is diluted 1:500 in BSA/PBS-T. For IF (cells were fixed in 2% formaldehyde) we had to dilute the antibody 1:50 to achieve a good cytoplasmic staining.
Date published: 2020-01-09
Rated 5 out of 5 by from SC-13132The antibody shows sensitive and specific in IHC.
Date published: 2019-11-21
Rated 5 out of 5 by from perfettoho provato l'anticorpo come campione gratuito. L'ho usato in western blot su campioni di cellule di ratto, diluito 1:1000 in BSA 5%.
Date published: 2019-10-31
Rated 5 out of 5 by from western blottingWorked well with western blotting at 1:600 dilution in HepG2 Cells.
Date published: 2018-12-24
Rated 3 out of 5 by from WB in HeLa, KM-12 and LIM1215Works fine with HeLa (50 µg) but no signal in other tested cell lines, Tested both 1:1000 and 1:500 dilutions in 5% Milk/TBST giving a little bit of background.
Date published: 2018-11-26
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HSP 27 抗体 (F-4) is rated 4.8 out of 5 by 37.
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