Date published: 2025-9-8

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HSL 抗体 (G-7): sc-74489

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说明书
  • HSL 抗体 G-7 是小鼠单克隆 IgG2b κ,HSL抗体, 在30篇文献中引用,规格为200 µg/ml
  • HSL 抗体 (G-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 HSL
  • HSL抗体(G-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSL (G-7): sc-74489免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 HSL 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HSL 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

HSL抗体(G-7)是一种鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类来源的HSL蛋白。抗HSL抗体(G-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。HSL蛋白(即激素敏感脂肪酶)在脂质代谢中起着至关重要的作用,它催化甘油三酯水解为游离脂肪酸,而游离脂肪酸对于能量产生至关重要,尤其是在脂肪组织和心脏组织中。HSL参与类固醇生成组织(如小肠)中胆固醇酯向游离胆固醇的转化,从而促进类固醇激素的合成。 HSL蛋白主要位于细胞质中,具有独特的表达模式,由可替代的翻译起始密码子产生两种主要的同工型;短同工型主要存在于脂肪组织中,而长同工型(通常称为睾丸HSL)则存在于类固醇生成组织(如睾丸)中。长异构体包含一个额外的N端区域,约有300个氨基酸,而短异构体则没有,这突出了HSL在不同组织中的结构多样性和功能专一性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSL 抗体 (G-7) 参考文献:

  1. 激素敏感脂肪酶以寡聚体的形式发挥作用。  |  Shen, WJ., et al. 2000. Biochemistry. 39: 2392-8. PMID: 10694408
  2. 豚鼠出生后发育过程中睾丸激素敏感脂肪酶的表达, 活性和亚细胞定位。  |  Kabbaj, O., et al. 2001. Biol Reprod. 65: 601-12. PMID: 11466232
  3. 激素敏感脂肪酶是肠粘膜的一种胆固醇酯化酶。  |  Grober, J., et al. 2003. J Biol Chem. 278: 6510-5. PMID: 12482847
  4. 激素敏感性脂肪酶 HSLtes 的睾丸形式可挽救 HSL 缺陷小鼠的雄性不育症。  |  Vallet-Erdtmann, V., et al. 2004. J Biol Chem. 279: 42875-80. PMID: 15292223
  5. 睾酮会影响左心室中激素敏感脂肪酶(HSL)的活性和脂质代谢。  |  Langfort, J., et al. 2010. Biochem Biophys Res Commun. 399: 670-6. PMID: 20691154
  6. 激素敏感脂肪酶(HSL)在哺乳动物脂肪分解过程中的复苏。  |  Lampidonis, AD., et al. 2011. Gene. 477: 1-11. PMID: 21241784
  7. 细胞外巨噬细胞迁移抑制因子(MIF)可下调脂肪荷尔蒙敏感性脂肪酶(HSL)并导致肥胖。  |  Chen, L., et al. 2024. Mol Metab. 79: 101834. PMID: 37935315
  8. p21 激活激酶 4 通过磷酸化 FABP4 和 HSL 来抵消 PKA 依赖性脂肪分解。  |  Yu, HC., et al. 2024. Nat Metab. 6: 94-112. PMID: 38216738
  9. 人类荷尔蒙敏感脂肪酶的基因组织和一级结构:与南极细菌莫拉菌 TA144 的脂肪酶序列同源性的可能意义。  |  Langin, D., et al. 1993. Proc Natl Acad Sci U S A. 90: 4897-901. PMID: 8506334
  10. 激素敏感脂肪酶睾丸同工型的分子克隆, 基因组组织和表达。  |  Holst, LS., et al. 1996. Genomics. 35: 441-7. PMID: 8812477
  11. 骨骼肌中对激素敏感的脂肪酶(HSL)的表达和调节。  |  Langfort, J., et al. 1998. Adv Exp Med Biol. 441: 219-28. PMID: 9781328
  12. 人类脂肪细胞激素敏感脂肪酶启动子的特征。  |  Grober, J., et al. 1997. Biochem J. 328 (Pt 2): 453-61. PMID: 9371701

订购信息

产品名称产品编号规格价格数量收藏夹

HSL 抗体 (G-7)

sc-74489
200 µg/ml
RMB2377.00

HSL (G-7): m-IgG Fc BP-HRP 套装

sc-525721
200 µg Ab; 10 µg BP
RMB2662.00

HSL (G-7): m-IgG2b BP-HRP 套装

sc-549629
200 µg Ab; 10 µg BP
RMB2662.00

HSL 抗体 (G-7) AC

sc-74489 AC
500 µg/ml, 25% agarose
RMB3129.00

HSL 抗体 (G-7) HRP

sc-74489 HRP
200 µg/ml
RMB2377.00

HSL 抗体 (G-7) FITC

sc-74489 FITC
200 µg/ml
RMB2482.00

HSL 抗体 (G-7) PE

sc-74489 PE
200 µg/ml
RMB2580.00

HSL 抗体 (G-7) Alexa Fluor® 488

sc-74489 AF488
200 µg/ml
RMB2685.00

HSL 抗体 (G-7) Alexa Fluor® 546

sc-74489 AF546
200 µg/ml
RMB2685.00

HSL 抗体 (G-7) Alexa Fluor® 594

sc-74489 AF594
200 µg/ml
RMB2685.00

HSL 抗体 (G-7) Alexa Fluor® 647

sc-74489 AF647
200 µg/ml
RMB2685.00

HSL 抗体 (G-7) Alexa Fluor® 680

sc-74489 AF680
200 µg/ml
RMB2685.00

HSL 抗体 (G-7) Alexa Fluor® 790

sc-74489 AF790
200 µg/ml
RMB2685.00

I see that there are some papers used this HSL Antibody (G-7): sc-74489 to mice western blot, but this antibody just for human HSL, so I want to know is it work to mice HSL?

Asked by: YEYUYANG
Thank you for your question. Based on our tests with this antibody, we can only recommend it for detection of HSL of human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2021-06-25

Do you have clear evidence that sc-74489 reacts with rat HSL? Thank you

Asked by: CMartin
Thank you for your question. HSL Antibody (G-7): sc-74489 is only recommended for detection of HSL of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our tests with this antibody it is not suitable for detection of rat HSL.
Answered by: Tech Support Europe
Date published: 2020-11-27

I need to evaluate phosphorylated HSL. Please, what are the catalog number?

Asked by: jose tarcisio
Thank you for your question. Currently, we do not offer an anti-p-HSL at this time. sc-74489:HSL (G-7), however, will detect total protein.
Answered by: Technical Support
Date published: 2018-12-05

I am using HSL (G-7): sc-74489 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Buon anticorpoCaricati su gel 20µg di estratto proteico totale da adipociti umani differenziati. L'anticorpo è stato usato a una diluizione 1:1000 in latte al 5%.
Date published: 2021-04-23
Rated 4 out of 5 by from Good featuresI bought this two weeks ago, I did Western Blot(1:100), there were two bands
Date published: 2017-07-18
Rated 5 out of 5 by from Good WB data with Rexwe bought it recently,tested in WB with Rax Heart,got better good data,very well thanks
Date published: 2016-12-27
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from Nice bands seen in WB using BTNice bands seen in WB using BT-20 and SK-BR-3 whole cell lysates. -SCBT QC
Date published: 2015-02-08
Rated 4 out of 5 by from Publishable data in WB using rat epididymalPublishable data in WB using rat epididymal adipose tissue -SCBT Publication Review
Date published: 2015-01-23
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HSL 抗体 (G-7) is rated 4.5 out of 5 by 6.
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