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HSF1 Antibody (10H8) - Western Blotting - Image 360973
HSF1 抗体 (10H8): sc-13516. HeLa (A), MCF7 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 HSF1 表达的 Western 印迹分析.
HSF1 抗体 (10H8): sc-13516. HeLa (A), MCF7 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 HSF1 表达的 Western 印迹分析.

HSF1 抗体 (10H8): sc-13516

4.6(11)
寫評論提問

说明书
  • HSF1 抗体 10H8 是大鼠单克隆 IgG1, 在18篇文献中引用，提供 200 µg/ml
  • 针对mouse起源的重组HSF1，其表位与氨基酸378-395对应
  • 抗-HSF1 抗体 (10H8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 HSF1
  • 抗HSF1抗体（10H8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13516 X, 200 µg/0.1 ml)
  • 关于如何获取HSF1 (10H8): sc-13516的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成HSF1 抗体 (10H8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HSF 抗体 包括 HSF1, HSF2, HSF4和HSF2BP

HSF1抗体（10H8）是一种鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）等方法检测小鼠、大鼠和人类样本中的HSF1。HSF1 (10H8) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。HSF1（即热休克因子1）通过调节热休克蛋白的表达，在细胞应激反应中发挥着至关重要的作用。热休克蛋白对于蛋白质折叠和防止细胞损伤至关重要。在正常情况下，HSF1主要位于细胞质中，以单体形式存在；然而，在应激条件下，HSF1会发生构象变化，从而形成三聚体并转移至细胞核。这种转移使HSF1能够与目标基因启动子区域中的热休克元件结合，从而启动热休克蛋白的转录，帮助在压力条件下维持细胞内环境稳定。HSF1的调控受翻译后修饰的影响，例如磷酸化，磷酸化可以激活或抑制转录活性，这凸显了精确控制HSF1在细胞应激反应中的功能的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

HSF1 抗体 (10H8)

sc-13516
200 µg/ml
RMB2377.00

HSF1 抗体 (10H8) X

sc-13516 X
200 µg/0.1 ml
RMB2377.00

HSF1 抗体 (10H8) AC

sc-13516 AC
500 µg/ml, 25% agarose
RMB3129.00

HSF1 抗体 (10H8) HRP

sc-13516 HRP
200 µg/ml
RMB2377.00

HSF1 抗体 (10H8) FITC

sc-13516 FITC
200 µg/ml
RMB2482.00

HSF1 抗体 (10H8) PE

sc-13516 PE
200 µg/ml
RMB2580.00

HSF1 抗体 (10H8) Alexa Fluor® 488

sc-13516 AF488
200 µg/ml
RMB2685.00

HSF1 抗体 (10H8) Alexa Fluor® 546

sc-13516 AF546
200 µg/ml
RMB2685.00

HSF1 抗体 (10H8) Alexa Fluor® 594

sc-13516 AF594
200 µg/ml
RMB2685.00

HSF1 抗体 (10H8) Alexa Fluor® 647

sc-13516 AF647
200 µg/ml
RMB2685.00

HSF1 抗体 (10H8) Alexa Fluor® 680

sc-13516 AF680
200 µg/ml
RMB2685.00

HSF1 抗体 (10H8) Alexa Fluor® 790

sc-13516 AF790
200 µg/ml
RMB2685.00

To whom it may concern, I have used this antibody in previous experiments and would just like to know if you can provide any information as to what each of the three bands revealed through Western Blot correspond to in reference to the HSF1 protein?

Asked by: rejimenez3
Thank you for your question. Unfortunately, we have not further characterized the identity of separate bands of similar molecular weight that we observed when testing this antibody in different cell lines. Representative Western blot data is shown on our website for this HSF1 antibody. According to Uniprot, there are 2 isoforms described with predicted molecular weights of ~ 57 and 53 kDa, however due to post-translational modifications the observed molecular weight for HSF1 protein is higher and typically greater than 90 kDa.
Answered by: Technical Support
Date published: 2020-04-15

When should the TransCruz reagent sc-13516 X be used instead of the standard form sc-13516?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Ok en IF et IHC cortex embryonnaireJ'ai testé cet anticorps et il fonctionne en IHC (1/50) CTX E11/E16 et en IF
Date published: 2018-06-08
Rated 4 out of 5 by from work well for wb1:200 diluted for wb of rat protein,the band is strong
Date published: 2018-04-21
Rated 4 out of 5 by from Immunofluorescence OKOK sur en IF coupe de cortex embryonnaire de souris.
Date published: 2018-04-16
Rated 5 out of 5 by from Great stainingExcellent choice for IHC or IF, does well with HRP and fluorescent secondaries and a variety of fixations including methanol and FFPE.
Date published: 2017-01-27
Rated 5 out of 5 by from Good publishable data in WB in rabbit aortaGood publishable data in WB in rabbit aorta. -SCBT Publication Review
Date published: 2015-05-16
Rated 5 out of 5 by from Strong band in WB in human HeLa and SCC7 cellsStrong band in WB in human HeLa and SCC7 cells. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of 5 by from Worked well in WB in human transfected 293TWorked well in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-02
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HSF1 抗体 (10H8) is rated 4.6 out of 5 by 11.
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