Date published: 2025-9-8
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HRI Antibody (D-12) - Western Blotting - Image 356989
HRI 抗体 (D-12): sc-365239. CCRF-CEM (A), HeLa (B), RAW 264. 7 (C), WEHI-231 (D), KNRK (E) 和 C6 (F) 全细胞裂解液中 HRI 表达的 Western 印迹分析.
HRI 抗体 (D-12): sc-365239. CCRF-CEM (A), HeLa (B), RAW 264. 7 (C), WEHI-231 (D), KNRK (E) 和 C6 (F) 全细胞裂解液中 HRI 表达的 Western 印迹分析.

HRI 抗体 (D-12): sc-365239

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说明书
  • HRI 抗体 D-12 是小鼠单克隆 IgG2a κ，HRI抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列27-165位于human物种的HRI的N-terminus附近
  • HRI 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HRI
  • 抗HRI抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HRI (D-12): sc-365239的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是HRI Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HRI Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

HRI抗体（D-12）是一种IgG2a κ小鼠单克隆HRI抗体（也称为HRI抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的HRI蛋白。HRI抗体（D-12）既有非偶联抗HRI抗体形式，也有多种偶联形式的抗HRI抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HRI（血红素调节抑制激酶）使eIF2alpha激酶的α亚基磷酸化，这在血红素缺乏时的翻译调控中起重要作用。HRI在多种环境条件下被激活，包括血红素缺乏、热休克和氧化应激。自磷酸化对于HRI的激活至关重要，这会导致蛋白质合成的起始受阻。HSP 90和HSC 70对于所有应激诱导的HRI激活都是必需的。此外，HSC 70对于将HRI折叠并转化为活性激酶是必需的，随后需要负向衰减转化HRI的激活。HRI特有的N末端和激酶插入结构域都参与血红素的结合和HRI的血红素调节。人类HRI基因位于7p22染色体上，编码一种主要在红系细胞中表达的630个氨基酸的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HRI 抗体 (D-12) 参考文献:

  1. Hsc70 在真核翻译起始因子 2 α 亚基血红素调节激酶的生物形成和调控中的双重作用。  |  Uma, S., et al. 1999. Mol Cell Biol. 19: 5861-71. PMID: 10454533
  2. 血红素调控真核生物启动因子-2α激酶的两个血红素结合域。N 末端和激酶插入。  |  Rafie-Kolpin, M., et al. 2000. J Biol Chem. 275: 5171-8. PMID: 10671563
  3. 克隆人类血红素调节真核细胞启动因子 2α 激酶 hHRI：在上皮性卵巢癌中下调。  |  Hwang, SY., et al. 2000. Mol Cells. 10: 584-91. PMID: 11101152
  4. 红细胞在细胞质压力下由血红素调控的真核生物起始因子 2α 激酶控制翻译起始。  |  Lu, L., et al. 2001. Mol Cell Biol. 21: 7971-80. PMID: 11689689
  5. 缺铁时红细胞前体的翻译调节和存活需要血红素调节的 eIF2alpha 激酶（HRI）。  |  Han, AP., et al. 2001. EMBO J. 20: 6909-18. PMID: 11726526
  6. 小鼠血红素调控的 eIF2alpha 激酶启动子的分子克隆和表征。  |  Lu, L. and Chen, JJ. 2002. Biochim Biophys Acta. 1574: 193-9. PMID: 11955629
  7. 有毒重金属离子通过抑制补充了血红素的网状细胞裂解液还原二硫键的能力，激活血红素调控的真核生物启动因子-2α激酶。  |  Matts, RL., et al. 1991. J Biol Chem. 266: 12695-702. PMID: 1676400
  8. 线粒体压力通过 OMA1-DELE1-HRI 途径传递到细胞质。  |  Guo, X., et al. 2020. Nature. 579: 427-432. PMID: 32132707
  9. 先天性免疫、蛋白稳态和线粒体应激中的 eIF2α 激酶 HRI。  |  Girardin, SE., et al. 2021. FEBS J. 288: 3094-3107. PMID: 32892501
  10. 综合应激反应的 HRI 分支可选择性地触发有丝分裂。  |  Chakrabarty, Y., et al. 2024. Mol Cell. 84: 1090-1100.e6. PMID: 38340717
  11. 线粒体蛋白导入应激过程中 HtrA2 的细胞膜滞留会触发 DELE1-HRI 通路。  |  Bi, PY., et al. 2024. Commun Biol. 7: 391. PMID: 38555279
  12. 血红素调节的真核生物启动因子 2 alpha 激酶的磷酸化与活性之间的关系。  |  Fagard, R. and London, IM. 1981. Proc Natl Acad Sci U S A. 78: 866-70. PMID: 6940153

订购信息

产品名称产品编号规格价格数量收藏夹

HRI 抗体 (D-12)

sc-365239
200 µg/ml
RMB2377.00

HRI (D-12): m-IgGκ BP-HRP 套装

sc-522173
200 µg Ab, 40 µg BP
RMB2662.00

HRI (D-12): m-IgG2a BP-HRP 套装

sc-546112
200 µg Ab; 10 µg BP
RMB2662.00

HRI 抗体 (D-12) AC

sc-365239 AC
500 µg/ml, 25% agarose
RMB3129.00

HRI 抗体 (D-12) HRP

sc-365239 HRP
200 µg/ml
RMB2377.00

HRI 抗体 (D-12) FITC

sc-365239 FITC
200 µg/ml
RMB2482.00

HRI 抗体 (D-12) PE

sc-365239 PE
200 µg/ml
RMB2580.00

HRI 抗体 (D-12) Alexa Fluor® 488

sc-365239 AF488
200 µg/ml
RMB2685.00

HRI 抗体 (D-12) Alexa Fluor® 546

sc-365239 AF546
200 µg/ml
RMB2685.00

HRI 抗体 (D-12) Alexa Fluor® 594

sc-365239 AF594
200 µg/ml
RMB2685.00

HRI 抗体 (D-12) Alexa Fluor® 647

sc-365239 AF647
200 µg/ml
RMB2685.00

HRI 抗体 (D-12) Alexa Fluor® 680

sc-365239 AF680
200 µg/ml
RMB2685.00

HRI 抗体 (D-12) Alexa Fluor® 790

sc-365239 AF790
200 µg/ml
RMB2685.00

HRI (D-12): sc-365239の無料の10 µgサンプルを頂きたいです。また、できればポジティブコントロールとなるようなデータシートでも使われているCCRF-CEM (A), HeLa (B), RAW 264.7 (C), WEHI-231 (D), KNRK (E) and C6 (F) whole cell lysatesの細胞サンプルも送って頂けますと幸いです。

Asked by: hayashi
ご質問ありがとうございます。抗体の購入とサンプル品の申請は日本のディーラーにお尋ねお願いします。宜しくお願い致します。 Thank you for your question. Please ask your Japanese dealer to purchase the antibodies and apply for the sample products. Thank you.
Answered by: Jiajun Z
Date published: 2024-08-26

What is the recommended fixation method for immunofluorescence staining with HRI (D-12): sc-365239 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2024-11-03
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Rated 1 out of 5 by from disappointedIt didn’t work for Western Blotting at the dilution of 1:500 overnight in the NB69 neuroblastoma cell line.
Date published: 2021-04-22
Rated 5 out of 5 by from Very good in WBWB on 10µg of total mouse heart protein extract. Gel 10%, single band detected at 1/1000 and 1/10000 for secondary Ab
Date published: 2017-10-21
Rated 3 out of 5 by from Western BlottingDetects a single band but the background is too high.
Date published: 2017-02-06
Rated 5 out of 5 by from Great for IF studiesThe HRI Antibody (D-12): sc-365239 works very well for IF, showing cytoplasmic localized staining pattern.
Date published: 2017-01-19
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. The 71 kDa target protein was apparently detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Great Western Blot data of HRI expressionGreat Western Blot data of HRI expression in Hep G2 and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-06-11
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HRI 抗体 (D-12) is rated 4.0 out of 5 by 7.
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