Date published: 2025-9-10
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
HPV16 E6/18 E6 Antibody (C1P5) - Western Blotting - Image 61230
用 HPV16 E6/18 E6 抗体 (C1P5): sc-460 免疫沉淀的 RKO E6 (A) 和 Ca Ski (B) 全细胞裂解液中 HPV18 E6 表达的 Western 印迹分析, 并用 HPV18 E6 (N-17): sc-1586 检测.
用 HPV16 E6/18 E6 抗体 (C1P5): sc-460 免疫沉淀的 RKO E6 (A) 和 Ca Ski (B) 全细胞裂解液中 HPV18 E6 表达的 Western 印迹分析, 并用 HPV18 E6 (N-17): sc-1586 检测.

HPV16 E6/18 E6 抗体 (C1P5): sc-460

3.7(27)
寫評論提問

说明书
  • HPV16 E6/18 E6 抗体 C1P5 是小鼠单克隆 IgG1 κ，HPV16 E6/18 E6抗体, 在104篇文献中引用，规格为200 µg/ml
  • 针对HPV E6
  • HPV16 E6/18 E6 抗体 (C1P5) 推荐用于 WB, IP, IF 和 IHC(P)，检测HPV 16 和HPV 18 来源的 the early protein E6 of Human Papillomavirus (HPV) types 16 and 18
  • 抗HPV16 E6/18 E6抗体（C1P5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HPV16 E6/18 E6 (C1P5): sc-460的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HPV16 E6/18 E6 抗体 (C1P5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HPV16 E6/18 E6 抗体 (C1P5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
说明书与实验方案
研究信息

関連項目

此外 HPV 抗体包括 HPV11 E7, HPV16 E1, HPV16 E18, HPV16 E2, HPV16 E4, HPV16 E6, HPV16 E7, HPV16 L1, HPV16 L2 和 HPV18 E2

HPV16 E6/18 E6抗体(C1P5)是一种IgG1 κ小鼠单克隆HPV16 E6/18 E6抗体(也称为人乳头瘤病毒16型转化蛋白E6抗体、HPV16 E6抗体、人乳头瘤病毒18型转化蛋白E6抗体、HPV18 E6抗体、α乳头瘤病毒7 (HPV18) E6蛋白抗体、α乳头瘤病毒9 (HPV16) E6蛋白抗体、HpV16gp1 (E6)抗体或HpV18gp1 (E8)抗体)，可通过WB、IP、IF和IHC(P)检测HPV 16和HPV 18来源的HPV16 E6/18 E6蛋白。HPV16 E6/18 E6抗体(C1P5)既可作为非偶联抗HPV16 E6/18 E6抗体形式，也可作为多种偶联形式的抗HPV16 E6/18 E6抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。人类乳头瘤病毒(HPVs)根据其与癌症的关联可分为高危型和低危型。HPV16和HPV18是高危组中最常见的，而HPV6和HPV11是低危组中的。大约90%的宫颈癌含有高危型HPV DNA。突变分析表明，高危型HPVs的E6和E7基因对于HPV转化功能是必要且充分的。E6和E7蛋白分别与p53和pRB的特异性相互作用与HPV高危和低危分类相关。高危型HPV E7蛋白与pRB的结合亲和力高于低危型HPV蛋白，且只有高危型HPV E6蛋白在体外与p53形成可检测复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HPV16 E6/18 E6 抗体 (C1P5) 参考文献:

  1. 早期浸润性宫颈癌的不良预后与未检测到 HPV DNA 之间的缔合作用。  |  Riou, G., et al. 1990. Lancet. 335: 1171-4. PMID: 1971033
  2. 人类乳头瘤病毒 16 型和 18 型 E6 蛋白与 p53 的关联。  |  Werness, BA., et al. 1990. Science. 248: 76-9. PMID: 2157286
  3. 人类乳头瘤病毒 16 型的 E6 和 E7 基因共同作用，是原代人类角质细胞转化的必要条件和充分条件。  |  Münger, K., et al. 1989. J Virol. 63: 4417-21. PMID: 2476573
  4. HPV16 E6 和 E6AP 在刺激或增强 Wnt 信号转导方面存在不同程度的合作。  |  Sominsky, S., et al. 2014. Virology. 468-470: 510-523. PMID: 25262469
  5. HPV16 E6 和 E7 蛋白合作使人类包皮角质细胞永生。  |  Hawley-Nelson, P., et al. 1989. EMBO J. 8: 3905-10. PMID: 2555178
  6. 人类乳头瘤病毒 E7 蛋白与视网膜母细胞瘤抑癌基因产物形成复合物。  |  Münger, K., et al. 1989. EMBO J. 8: 4099-105. PMID: 2556261
  7. 无癌症男性中口腔、肛门或生殖器 HPV16 感染的 HPV16 E6 血清阳性率。  |  Beachler, DC., et al. 2016. Papillomavirus Res. 2: 141-144. PMID: 28239675
  8. HPV16 E6 mRNA或E7 mRNA定量表达对宫颈细胞不典型增生和癌变的诊断作用。  |  Wu, MZ., et al. 2018. Cell Transplant. 27: 1401-1406. PMID: 30056761
  9. 人类MHC-I HPV18 E6/E7特异性CD8+ T细胞表位的鉴定以及HLA-A2转基因小鼠中HPV18 E6/E7表达腺鳞癌的生成。  |  Peng, S., et al. 2022. J Biomed Sci. 29: 80. PMID: 36224625
  10. HPV16 E6诱导染色体不稳定性，这是由于E6AP依赖性有丝分裂驱动蛋白CENP-E降解引起的极性染色体。  |  Cosper, PF., et al. 2023. Proc Natl Acad Sci U S A. 120: e2216700120. PMID: 36989302
  11. 有两种不同的机制可以调节细胞肿瘤抗原 p53 的水平。  |  Reich, NC., et al. 1983. Mol Cell Biol. 3: 2143-50. PMID: 6318085
  12. 克隆和表达 E6-AP 的 cDNA，E6-AP 是一种介导人类乳头瘤病毒 E6 肿瘤蛋白与 p53 相互作用的蛋白质。  |  Huibregtse, JM., et al. 1993. Mol Cell Biol. 13: 775-84. PMID: 8380895

订购信息

产品名称产品编号规格价格数量收藏夹

HPV16 E6/18 E6 抗体 (C1P5)

sc-460
200 µg/ml
RMB2377.00

HPV16 E6/18 E6 (C1P5): m-IgG Fc BP-HRP 套装

sc-528112
200 µg Ab; 10 µg BP
RMB2662.00

HPV16 E6/18 E6 (C1P5): m-IgGκ BP-HRP 套装

sc-520412
200 µg Ab, 40 µg BP
RMB2662.00

HPV16 E6/18 E6 (C1P5): m-IgG1 BP-HRP 套装

sc-542766
200 µg Ab; 20 µg BP
RMB2662.00

HPV16 E6/18 E6 抗体 (C1P5) AC

sc-460 AC
500 µg/ml, 25% agarose
RMB3129.00

HPV16 E6/18 E6 抗体 (C1P5) HRP

sc-460 HRP
200 µg/ml
RMB2377.00

HPV16 E6/18 E6 抗体 (C1P5) FITC

sc-460 FITC
200 µg/ml
RMB2482.00

HPV16 E6/18 E6 抗体 (C1P5) PE

sc-460 PE
200 µg/ml
RMB2580.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 488

sc-460 AF488
200 µg/ml
RMB2685.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 546

sc-460 AF546
200 µg/ml
RMB2685.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 594

sc-460 AF594
200 µg/ml
RMB2685.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 647

sc-460 AF647
200 µg/ml
RMB2685.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 680

sc-460 AF680
200 µg/ml
RMB2685.00

HPV16 E6/18 E6 抗体 (C1P5) Alexa Fluor® 790

sc-460 AF790
200 µg/ml
RMB2685.00

Kindly suggest me the positive control for HPV 16/18 E6 ANTIBODY in IHC

Asked by: RAJESH
Thank you for your question. We recommend RKO E6 Whole Cell Lysate or Ca Ski Whole Cell Lysate.
Answered by: BlakeJ
Date published: 2024-06-13

I am a doctor in FL and would like to order HPV 16/18 E6 antibodies on my patients. Suggestions?

Asked by: JBDDOPA
Santa Cruz antibodies are research grade and not intended for diagnostic or therapeutic use.
Answered by: Technical Service
Date published: 2023-03-01

Is it going to stain HPV16/18 indiferently or is it possible to identify each one (HPV16 and HPV18)?

Asked by: murilo
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

I have run 3 times' western blot using this antibody. There is no 16/17 KD size show out. Instead, I have band show at the size about 70KD.

Asked by: Yifan
We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: Tech Service
Date published: 2022-10-05

It didn't detect at 16/17kDa size on HeLa, SiHa and Caski whole cell lysate for western blot (1:500 + 2nd 1:3000). This antibody I tried at this time is new one. (I've got same antibody, but also It didn't work for western blot (1:100~1:500).)

Asked by: YK N
Thank you for your question. Please contact your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2020-02-13

HPV16 E6 항체 (N-17): sc-1584 대체 상품으로 본 제품을 보고 있는데요, 항원으로 사용한 amino acid sequence 궁금합니다.

Asked by: keanu323
문의주셔서 감사합니다. 이 항체의 항원의 구체적인 amino acid sequence 정보는 제공할 수 없습니다.raised against HPV E6.Thanks for your question ,the antigen information we can provide is on Web,it is raised against HPV E6.You can also contact us at email : [email protected] or by live chat for pther questions..
Answered by: Technical Service 11
Date published: 2020-01-03

Recently I have bought HPV16/18E6 antibody sc-460. Western blot was performed for this antibody various dilution ranging from 1:100 to 1:500. But I could not get any band at 17kDa. But there were non specific bands from 35kda till 63kDa. Please guide me.

Asked by: Reshma
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-05-20

Hello, Could we know the AA sequence used to elaborate the antibody ? Or perhaps the epitope location in the sequence (N-ter or C-ter ?)

Asked by: carcinoHPVFR
Thank you for your question. This antibody was raised against HPV E6. For additional information, please contact our Technical Service https://www.scbt.com/scbt/resources/technical-service
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_10
  • loc_zh_CN, sid_460, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from It doesn't work on Hela lysatesI have tried more than 5 times to detect E6 with the HeLa extract, I have tried with different dilutions, and any specific band is detected. There are a lot of unspecificities.
Date published: 2023-06-21
Rated 1 out of 5 by from no target bandsThere was no target band between 15-25kda marker for Hela cell lysate. I have repeated it four times, but it still does not work.
Date published: 2021-11-20
Rated 1 out of 5 by from 전혀 반응하지 않음밴드 전혀 안나옵니다. 고생만 많이 하고. 이제 보니 최근 2년간 후기에는 밴드가 안보였다는 것만 있네요
Date published: 2021-10-08
Rated 1 out of 5 by from No band detectedUnfortunately, I do not detect any bands using this antibody. I have tested it 3x already, using Hela lysates (HPV18) and TC-1 lysates (HPV16), and no band is visible.
Date published: 2021-03-28
Rated 1 out of 5 by from 真是糟糕透了Hela和TC-1跑不出来，上样量30μg-200μg，稀释比例1：100-1:1000，都是只有在60Kd以上有杂带，但纯化后的蛋白上样量50ng能够跑出来，为此我还RT-PCR鉴定了我手里的细胞，测序结果没有任何问题，浪费的时间太多了，该抗体效价怎么能这么低。
Date published: 2020-06-18
Rated 1 out of 5 by from Does not work on Ca SkiI was looking for an antibody with which to detect E6 on both Ca Ski and HeLa cells. I tried it on Ca Ski whole cell lysates 3 times and it does not work.
Date published: 2020-01-16
Rated 5 out of 5 by from Great antibodyGreat antibody with clear bands and no background,very nice performance wih 1:400 to 1:800 diluted.
Date published: 2018-05-26
Rated 5 out of 5 by from 功能很出色我一个月前购买了此产品，实验很成功，图像很清晰，并为此感到很高兴，还会再来这儿购买其他产品的，继续努力
Date published: 2018-05-22
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_zh_CN, sid_460, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
HPV16 E6/18 E6 抗体 (C1P5) is rated 3.7 out of 5 by 27.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_zh_CN, sid_460, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。