Date published: 2025-9-7
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HP1γ Antibody (F-1) - Western Blotting - Image 305788
HP1γ 抗体 (F-1): sc-398562. HeLa (A) 和 K-562 (B) 核提取物以及 K-562 (C), HeLa (D) 和 MCF7 (E) 全细胞裂解液中 HP1γ 表达的 Western 印迹分析.
HP1γ 抗体 (F-1): sc-398562. HeLa (A) 和 K-562 (B) 核提取物以及 K-562 (C), HeLa (D) 和 MCF7 (E) 全细胞裂解液中 HP1γ 表达的 Western 印迹分析.

HP1γ 抗体 (F-1): sc-398562

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说明书
  • HP1 gamma 抗体 F-1 是小鼠单克隆 IgG2a κ，HP1γ抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-104位于human物种的HP1γ的N-terminus
  • HP1 gamma 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HP1γ
  • 抗HP1 gamma抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HP1γ (F-1): sc-398562的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 是HP1γ 抗体 (F-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与HP1γ 抗体 (F-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HP1 抗体 包括 HP1 α, HP1 β和HP1 γ

HP1γ抗体（F-1）是一种IgG2a κ型小鼠单克隆HP1 gamma抗体（也称为Chromobox 3抗体、Heterochromatin Protein 1 Homolog Gamma抗体、Chromobox Protein Homolog 3抗体、Modifier 2 Protein抗体、HP1Hs-Gamma抗体、HECH抗体、Heterochromatin Protein HP1 Gamma抗体、HP1 Gamma Homolog (Drosophila)抗体、Heterochromatin-Like Protein 1抗体、Chromobox Homolog 3抗体、HP1 Gamma Homolog抗体或HP1-GAMMA抗体），它可以通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附试验（ELISA）检测到源自小鼠、大鼠和人类的HP1 gamma蛋白。HP1γ抗体（F-1）既有非偶联型抗HP1 gamma抗体形式，也有多种偶联型抗HP1 gamma抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。染色质组装因子-1（CAF-1）是一个由三个多肽亚基（称为p150、p60和p48）组成的多亚基蛋白复合物。CAF-1是一种核小体组装因子，在DNA复制过程中将新合成的乙酰化组蛋白H3/H4沉积到新生的染色质中。CAF-1的p150亚基还支持异染色质的维持，这需要新组蛋白和异染色质蛋白的合成及其在DNA复制过程中的有序组装。异染色质的特征是密集卷曲的染色质，通常在S期晚期复制，基因密度低，含有大量相对不易被DNA修饰试剂接触的重复DNA。在S期晚期，p150直接与异染色质相关蛋白1（HP1α、HP1β和HP1γ）结合。当细胞准备进行有丝分裂时，CAF-1 p150和一些HP1逐渐从异染色质中解离，这与组蛋白H3的磷酸化同时发生。HP1蛋白在有丝分裂结束时与染色质重新结合，此时组蛋白H3被去磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HP1γ 抗体 (F-1) 参考文献:

  1. 哺乳动物细胞中 HP1 蛋白在细胞周期中的定位和磷酸化。  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
  2. 小鼠细胞中的异染色质动力学：染色质组装因子1和HP1蛋白之间的相互作用。  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
  3. 异染色质结构域的复制和维护  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
  4. 鉴定异染色质蛋白1（HP1）作为Pim-1激酶的磷酸化靶标以及磷酸化对HP1(1)转录抑制功能的影响。  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
  5. DNA 复制过程中染色质组装所需的体外人类细胞因子 CAF-I 的纯化和特征描述。  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
  6. 染色质组装因子 I 的 p150 和 p60 亚基：新合成组蛋白与 DNA 复制之间的分子联系。  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
  7. 通过 CAF-1 和乙酰化组蛋白 H3/H4 复合物组装核小体。  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

订购信息

产品名称产品编号规格价格数量收藏夹

HP1γ 抗体 (F-1)

sc-398562
200 µg/ml
RMB2377.00

HP1γ (F-1): m-IgG2a BP-HRP 套装

sc-545973
200 µg Ab; 10 µg BP
RMB2662.00

HP1γ 抗体 (F-1) AC

sc-398562 AC
500 µg/ml, 25% agarose
RMB3129.00

HP1γ 抗体 (F-1) HRP

sc-398562 HRP
200 µg/ml
RMB2377.00

HP1γ 抗体 (F-1) FITC

sc-398562 FITC
200 µg/ml
RMB2482.00

HP1γ 抗体 (F-1) PE

sc-398562 PE
200 µg/ml
RMB2580.00

HP1γ 抗体 (F-1) Alexa Fluor® 488

sc-398562 AF488
200 µg/ml
RMB2685.00

HP1γ 抗体 (F-1) Alexa Fluor® 546

sc-398562 AF546
200 µg/ml
RMB2685.00

HP1γ 抗体 (F-1) Alexa Fluor® 594

sc-398562 AF594
200 µg/ml
RMB2685.00

HP1γ 抗体 (F-1) Alexa Fluor® 647

sc-398562 AF647
200 µg/ml
RMB2685.00

HP1γ 抗体 (F-1) Alexa Fluor® 680

sc-398562 AF680
200 µg/ml
RMB2685.00

HP1γ 抗体 (F-1) Alexa Fluor® 790

sc-398562 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with HP1γ (F-1): sc-398562 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from worked for ARPE-19worked well for immune fluorescent staining with ARPE-19 and mouse retina
Date published: 2022-09-01
Rated 5 out of 5 by from worked well for IF in PFA fixed cellsI received the Ab as sample. Used at 1:100. Bright labeling confined to the nucleus as expected.
Date published: 2019-05-04
Rated 5 out of 5 by from Great antibody for IFI recently received the trial size of this antibody. It worked great for IF in my mouse cell line. Will post the images later.
Date published: 2018-01-31
Rated 5 out of 5 by from excellentThis secondary antibody works well when we use 1:2000 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-09-20
Rated 5 out of 5 by from an excellent antibody for both WB and IFThis antibody generates a clean and strong signal at the expected molecular weight (1:500 dilution, 40 ug of HeLa lysate). It also works well for IF in formaldehyde-fixed HeLa cells (1:200 dilution).
Date published: 2017-08-03
Rated 4 out of 5 by from Great immunohistochemical nuclear stainingGreat immunohistochemical nuclear staining in mouse glioma paraffin section.
Date published: 2017-01-20
Rated 5 out of 5 by from Worked great for WB !Worked great for WB with my cell line! It worked with concentration at 1:1000.
Date published: 2016-12-01
Rated 5 out of 5 by from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-19
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HP1γ 抗体 (F-1) is rated 4.8 out of 5 by 9.
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