Date published: 2025-9-6
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HP1α Antibody (GA-62) - Western Blotting - Image 149037
HP1α (GA-62): sc-130446. K-562 (A), MCF7 (B) 和 Jurkat (C) 核提取物中 HP1α 表达的 Western 印迹分析.
HP1α (GA-62): sc-130446. K-562 (A), MCF7 (B) 和 Jurkat (C) 核提取物中 HP1α 表达的 Western 印迹分析.

HP1α 抗体 (GA-62): sc-130446

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说明书
  • HP1 alpha 抗体 GA-62 是小鼠单克隆 IgG1 κ，HP1α抗体, 在5篇文献中引用，规格为100 µg/ml
  • 针对全长重组HP1α，human起源
  • 推荐用于 mouse, rat 和 human 来源的HP1α WB, IP, IF 和 ELISA检测
  • 关于如何获取HP1α (GA-62): sc-130446的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是HP1α Antibody (GA-62) for WB applications. 的首选辅助检测试剂，这些试剂现与HP1α Antibody (GA-62) 打包提供（请参阅下面的订购信息）。

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関連項目

额外HP1抗体，包括HP1 α, HP1 β和HP1 γ

HP1α 抗体（GA-62）是一种 IgG1 κ 小鼠单克隆 HP1 α 抗体（也称为 Chromobox 5 抗体、Chromobox Homolog 5 (HP1 Alpha Homolog Drosophila) 抗体、Heterochromatin Protein 1 Homolog Alpha 抗体、Antigen P25 抗体、HP1-ALPHA 抗体、HP1A 抗体、HP1 抗体、Chromobox Homolog 5 (Drosophila HP1 Alpha) 抗体、Heterochromatin Protein 1-Alpha 抗体、HP1Hs-Alpha 抗体或 HP1 Alpha 抗体），它通过WB, IP, IF和ELISA检测小鼠、大鼠和人的 HP1 α 蛋白。HP1α 抗体（GA-62）可作为非偶联的抗 HP1 α 抗体形式提供。染色质组装因子-1（CAF-1）是一个多亚基蛋白质复合物，由三个被称为 p150、p60 和 p48 的多肽亚基组成。CAF-1 是一种核小体组装因子，在 DNA 复制过程中将新合成和乙酰化的组蛋白 H3/H4 沉积到新生的染色质中。CAF-1 的 p150 亚基还支持异染色质的维持，这需要合成新的组蛋白和异染色质蛋白及其在 DNA 复制过程中的有序组装。异染色质的特征是紧密盘绕的染色质，通常在 S 期晚期复制，基因密度低，并含有大量相对不易被 DNA 修饰试剂接近的重复 DNA 片段。在 S 期晚期，p150 直接与异染色质相关蛋白 1（HP1α、HP1β 和 HP1γ）结合。随着细胞准备进行有丝分裂，CAF-1 p150 和一些 HP1 逐渐从异染色质中解离，与组蛋白 H3 的磷酸化同时发生。在有丝分裂结束时，随着组蛋白 H3 的去磷酸化，HP1 蛋白重新与染色质结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HP1α 抗体 (GA-62) 参考文献:

  1. 哺乳动物细胞中 HP1 蛋白在细胞周期中的定位和磷酸化。  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
  2. 小鼠细胞中的异染色质动力学：染色质组装因子1和HP1蛋白之间的相互作用。  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
  3. 异染色质结构域的复制和维护  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
  4. 鉴定异染色质蛋白1（HP1）作为Pim-1激酶的磷酸化靶标以及磷酸化对HP1(1)转录抑制功能的影响。  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
  5. DNA 复制过程中染色质组装所需的体外人类细胞因子 CAF-I 的纯化和特征描述。  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
  6. HP1&α形成液滴；表明相分离在异染色质中的作用。  |  Larson, AG., et al. 2017. Nature. 547: 236-240. PMID: 28636604
  7. MCF7细胞中HP1&α;耗竭时膜连接特性的变化。  |  Pradhan, S., et al. 2022. Biochem Biophys Res Commun. 587: 126-130. PMID: 34872000
  8. 磷酸化 HP1α- 核小体在相分离环境中的相互作用  |  Elathram, N., et al. 2023. J Am Chem Soc. 145: 23994-24004. PMID: 37870432
  9. 揭示 HP1α-HDAC1-STAT1 轴作为 HP1α- 阳性肝内胆管癌治疗靶点的作用。  |  Xiong, F., et al. 2024. J Exp Clin Cancer Res. 43: 152. PMID: 38812060
  10. HP1α/Swi6 的凝结增强了核硬度。  |  Williams, JF., et al. 2024. Cell Rep. 43: 114373. PMID: 38900638
  11. 染色质组装因子 I 的 p150 和 p60 亚基：新合成组蛋白与 DNA 复制之间的分子联系。  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
  12. 通过 CAF-1 和乙酰化组蛋白 H3/H4 复合物组装核小体。  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

订购信息

产品名称产品编号规格价格数量收藏夹

HP1α 抗体 (GA-62)

sc-130446
100 µg/ml
RMB2504.00

HP1α (GA-62): m-IgG Fc BP-HRP 套装

sc-539824
100 µg Ab; 10 µg BP
RMB2662.00

HP1α (GA-62): m-IgG1 BP-HRP 套装

sc-541731
100 µg Ab; 20 µg BP
RMB2662.00

I am using HP1α (GA-62): sc-130446 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from not very good for ARPE-19I tried to get good images but failed to get signal.
Date published: 2022-09-01
Rated 5 out of 5 by from Very good antibodyThis product works very well without aspecific bands for WB, with 1:500 dilution.
Date published: 2021-01-26
Rated 5 out of 5 by from an excellent antibody for WBThis antibody produces a strong and clean signal on WB (1:500 dilution, 40 ug of HeLa lysate). It may not work for IF in formaldehyde-fixed HeLa cells. We did not try methanol-fixed cells.
Date published: 2017-08-08
Rated 2 out of 5 by from not that goodI used this antibody for my CB/IP experiment. When I did the IB with this antibody I didn't get a band, tried using the same and different species as an IP. Didn't work for immunofluorescence.
Date published: 2017-03-09
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Strong bands seen in WB using KStrong bands seen in WB using K-562, MCF7 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-04-25
Rated 4 out of 5 by from Band detected in WB using HeLa nuclear extractBand detected in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-03-25
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-13
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HP1α 抗体 (GA-62) is rated 3.8 out of 5 by 8.
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