Date published: 2025-9-6
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hnRNP M1-4 Antibody (1D8) - Western Blotting - Image 373592
hnRNP M1-4 抗体 (1D8): sc-20002. HeLa (A), Jurkat (B) 和 K-562 (C) 全细胞裂解液及 NIH/3T3 核提取物 (D) 中 hnRNP M1-4 表达的 Western 印迹分析.
hnRNP M1-4 抗体 (1D8): sc-20002. HeLa (A), Jurkat (B) 和 K-562 (C) 全细胞裂解液及 NIH/3T3 核提取物 (D) 中 hnRNP M1-4 表达的 Western 印迹分析.

hnRNP M1-4 抗体 (1D8): sc-20002

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说明书
  • hnRNP M1-4抗体(1D8)是小鼠单克隆IgG1 κ, 在26篇文献中引用，规格为200 µg/ml
  • 源自Balb/c淋巴细胞，骨髓瘤亲本SP2/O（免疫原为融合蛋白），mouse来源
  • 抗-hnRNP M1-4 抗体 (1D8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 hnRNP M1, hnRNP M2, hnRNP M3 and hnRNP M4; 也和以下物种反应，包括： 和 rabbit, bovine and porcine
  • 抗hnRNP M1-4抗体（1D8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP M1-4 (1D8): sc-20002的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 hnRNP M1-4 抗体 (1D8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP M1-4 抗体 (1D8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 hnRNP 抗体 包括 hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2和hnRNP F

hnRNP M1-4抗体（1D8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）等方法检测小鼠、大鼠和人类样本中的hnRNP M1-4。（IHC）。hnRNP M1-4 (1D8) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor® 结合物。异质核糖核蛋白（hnRNPs）在基因表达调控中发挥着关键作用，特别是在mRNA处理和运输方面。特别是hnRNP M蛋白，它参与前mRNA剪接，并影响mRNA的稳定性和翻译 。已知hnRNP M1-4蛋白与其他剪接因子和RNA结合蛋白相互作用，这对于剪接体（负责从前mRNA中去除内含子的复合体）的正确组装至关重要。这种相互作用对于维持基因表达的准确性至关重要，因为剪接错误会导致功能失调的蛋白质的产生，并导致各种疾病。 hnRNP M蛋白能够在细胞核和细胞质之间穿梭的能力进一步凸显了它们在转录后调节中的重要性，这使得抗hnRNP M1-4单克隆抗体（1D8）成为研究人员研究RNA生物学及其对健康和疾病的影响的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP M1-4 抗体 (1D8) 参考文献:

  1. 核前 mRNA 区隔：将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  3. 70-110 S 大型异质核糖核蛋白复合体（LH-nRNP）的 72/74 kDa 多肽代表了 hnRNP M 蛋白家族的一个离散子集。  |  Kafasla, P., et al. 2000. Biochem J. 350 Pt 2: 495-503. PMID: 10947964
  4. 小鼠胚胎发育过程中 hnRNP M 和 2H9 基因表达的时空调控。  |  Mahé, D., et al. 2000. Biochim Biophys Acta. 1492: 414-24. PMID: 11004512
  5. 替代剪接抑制因子 hnRNP A1/A2 和 PTB 影响丙酮酸激酶同工酶的表达和细胞代谢。  |  Clower, CV., et al. 2010. Proc Natl Acad Sci U S A. 107: 1894-9. PMID: 20133837
  6. hnRNP M 同工型与 TET 蛋白 TAF15 和 TLS/FUS 的选择性相互作用。  |  Marko, M., et al. 2014. Mol Biol Rep. 41: 2687-95. PMID: 24474660
  7. LONRF2 是一种蛋白质质量控制泛素连接酶，其缺乏会导致晚发性神经系统缺陷。  |  Li, D., et al. 2023. Nat Aging. 3: 1001-1019. PMID: 37474791
  8. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  9. 人类 hnRNP M 蛋白：核糖核蛋白中富含蛋氨酸/精氨酸的重复图案的鉴定。  |  Datar, KV., et al. 1993. Nucleic Acids Res. 21: 439-46. PMID: 8441656
  10. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  11. 人类 hnRNP-M 蛋白：结构及与早期热休克诱导的剪接停滞和染色体图谱的关系。  |  Gattoni, R., et al. 1996. Nucleic Acids Res. 24: 2535-42. PMID: 8692693
  12. 红细胞分化过程中的 mRNA 沉默：hnRNP K 和 hnRNP E1 从 3' 端调控 15 脂氧合酶的翻译。  |  Ostareck, DH., et al. 1997. Cell. 89: 597-606. PMID: 9160751

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP M1-4 抗体 (1D8)

sc-20002
200 µg/ml
RMB2377.00

hnRNP M1-4 (1D8): m-IgG Fc BP-HRP 套装

sc-528347
200 µg Ab; 10 µg BP
RMB2662.00

hnRNP M1-4 (1D8): m-IgGκ BP-HRP 套装

sc-520701
200 µg Ab, 40 µg BP
RMB2662.00

hnRNP M1-4 (1D8): m-IgG1 BP-HRP 套装

sc-542882
200 µg Ab; 20 µg BP
RMB2662.00

hnRNP M1-4 抗体 (1D8) AC

sc-20002 AC
500 µg/ml, 25% agarose
RMB3129.00

hnRNP M1-4 抗体 (1D8) HRP

sc-20002 HRP
200 µg/ml
RMB2377.00

hnRNP M1-4 抗体 (1D8) FITC

sc-20002 FITC
200 µg/ml
RMB2482.00

hnRNP M1-4 抗体 (1D8) PE

sc-20002 PE
200 µg/ml
RMB2580.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 488

sc-20002 AF488
200 µg/ml
RMB2685.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 546

sc-20002 AF546
200 µg/ml
RMB2685.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 594

sc-20002 AF594
200 µg/ml
RMB2685.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 647

sc-20002 AF647
200 µg/ml
RMB2685.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 680

sc-20002 AF680
200 µg/ml
RMB2685.00

hnRNP M1-4 抗体 (1D8) Alexa Fluor® 790

sc-20002 AF790
200 µg/ml
RMB2685.00

Hi, may I know what is the epitope (i.e. which amino acid residues) on hnRNPM that this antibody binds to/was raised against? Thanks!

Asked by: Faye12345
Derived from lymphoid cell obtained from Balb/c and with myeloma parent SP2/O (immunogen is a fusion protein) of mouse origin.
Answered by: Technical Service
Date published: 2023-09-14

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 5 out of 5 by from Good for WBI used this ab for WB in my paper. It gives good result. I would like to recommend this product to the community.
Date published: 2020-11-13
Rated 5 out of 5 by from Excellent antibody for WB20ug of whole cell lysates made from MDA-MB231 cells, probed at 1/200X dilution. Signal was clean and minimal background.
Date published: 2020-08-14
Rated 5 out of 5 by from Great antibodyThe antibody works very well, I am very satisfied.
Date published: 2018-09-25
Rated 5 out of 5 by from Good signal for Western Blot and IPI used this antibody for western blot on whole cell lysate as well as nuclear fractions in MDAMB231 cells and it works very well. block 1 hr in 5% milk primary antibody: 1:1000 in 5% milk overnight secondary antibody: 1:5000 in 5% milk 1 hr Works well for IP as well. I collected the nuclear fraction of MDAMB231 ce using 25ul of antibody for 1 15cm dish ~90% confluent.
Date published: 2018-09-12
Rated 5 out of 5 by from excellenWe used this product in WB assay, It is very clear in western blot, very good!
Date published: 2017-05-13
Rated 5 out of 5 by from The antibody is wonderfulGood quality for western blotting with NIH/3T3 nuclear extract detection, dilution with 1:300
Date published: 2017-04-24
Rated 5 out of 5 by from Great IF stainingWe used this antibody for IF staining and got great intensity and a clear image from nuclear regions
Date published: 2017-01-09
Rated 5 out of 5 by from Good publishable data in WB in human HEK 293 cellsGood publishable data in WB in human HEK 293 cells. -SCBT Publication Review
Date published: 2015-03-26
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hnRNP M1-4 抗体 (1D8) is rated 5.0 out of 5 by 12.
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