Date published: 2025-9-6
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hnRNP F/H Antibody (1G11) - Immunohistochemistry - Image 284776
hnRNP F/H 抗体 (1G11): sc-32310. 福尔马林固定, 石蜡包埋的人十二指肠组织的免疫过氧化物酶染色显示腺细胞的核染色.
hnRNP F/H 抗体 (1G11): sc-32310. 福尔马林固定, 石蜡包埋的人十二指肠组织的免疫过氧化物酶染色显示腺细胞的核染色.

hnRNP F/H 抗体 (1G11): sc-32310

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寫評論提問

说明书
  • hnRNP F/H抗体(1G11)是小鼠单克隆IgG1 λ hnRNP F/H抗体, 在18篇文献中引用，规格为200 µg/ml
  • 针对human来源的纯化重组hnRNP F/H
  • hnRNP F/H 抗体 (1G11) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的hnRNP F and hnRNP H
  • 抗hnRNP F/H抗体（1G11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP F/H (1G11): sc-32310的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 hnRNP F/H 抗体 (1G11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与hnRNP F/H 抗体 (1G11) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品，请参阅我们的 小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 hnRNP 抗体 包括 hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2和hnRNP F

hnRNP F/H抗体（1G11）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的hnRNP F/H蛋白。抗hnRNP 抗体（1G11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。异质核糖核蛋白（hnRNPs）在预mRNA处理和运输中起着至关重要的作用，与RNA聚合酶II产生的异质核RNA（hnRNA）结合。其中，hnRNP F和H尤为重要，因为它们优先与RNA上的聚（rG）区域结合，影响基因表达的调节。hnRNP F和H蛋白参与hnRNP复合物的形成， 是剪接体的重要组成部分，并与其他剪接因子相互作用，以调节前体mRNA上的剪接位点选择。hnRNP F和H经过翻译后修饰，包括磷酸化作用和甲基化作用，这些修饰会影响其功能以及与其他蛋白的相互作用。了解hnRNP F和H的动态变化，包括相互作用和修饰，对于阐明其在RNA代谢和基因表达调控中的作用至关重要，这使得hnRNP F/H（1G11）单克隆抗体成为无价的研究工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP F/H 抗体 (1G11) 参考文献:

  1. 核前 mRNA 区隔：将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  3. hnRNP A/B 和 hnRNP F/H 蛋白的内联结合位点刺激前 mRNA 剪接。  |  Martinez-Contreras, R., et al. 2006. PLoS Biol. 4: e21. PMID: 16396608
  4. hnRNP H 和 hnRNP F 与 Fox2 复合，使成纤维细胞生长因子受体 2 外显子 IIIc 沉默。  |  Mauger, DM., et al. 2008. Mol Cell Biol. 28: 5403-19. PMID: 18573884
  5. 布氏锥虫的 hnRNP F/H 同源物在寄生虫的两个生命周期阶段中表达不同，并调控剪接和 mRNA 的稳定性。  |  Gupta, SK., et al. 2013. Nucleic Acids Res. 41: 6577-94. PMID: 23666624
  6. 细胞质震颤 I 同工型调节脊髓神经胶质细胞中 hnRNP F/H 依赖性替代剪接途径。  |  Mandler, MD., et al. 2014. Nucleic Acids Res. 42: 7319-29. PMID: 24792162
  7. HnRNP F/H 与 hTERC 和端粒酶全酶结合，调节端粒酶功能，促进细胞增殖。  |  Xu, C., et al. 2020. Cell Death Differ. 27: 1998-2013. PMID: 31863069
  8. 一种新型低丰度 hnRNP 蛋白的分离与鉴定：A0.  |  Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
  9. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  10. 章鱼 RNP 蛋白家族的三个新成员。  |  Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
  11. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  12. 颉颃物前核糖核酸剪接因子的组织特异性调控表达。  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP F/H 抗体 (1G11)

sc-32310
200 µg/ml
RMB2377.00

hnRNP F/H (1G11): m-IgGλ BP-HRP 套装

sc-520839
200 µg Ab, 40 µg BP
RMB2662.00

hnRNP F/H 抗体 (1G11) AC

sc-32310 AC
500 µg/ml, 25% agarose
RMB3129.00

hnRNP F/H 抗体 (1G11) HRP

sc-32310 HRP
200 µg/ml
RMB2377.00

hnRNP F/H 抗体 (1G11) FITC

sc-32310 FITC
200 µg/ml
RMB2482.00

hnRNP F/H 抗体 (1G11) PE

sc-32310 PE
200 µg/ml
RMB2580.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 488

sc-32310 AF488
200 µg/ml
RMB2685.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 546

sc-32310 AF546
200 µg/ml
RMB2685.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 594

sc-32310 AF594
200 µg/ml
RMB2685.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 647

sc-32310 AF647
200 µg/ml
RMB2685.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 680

sc-32310 AF680
200 µg/ml
RMB2685.00

hnRNP F/H 抗体 (1G11) Alexa Fluor® 790

sc-32310 AF790
200 µg/ml
RMB2685.00

Hi. I'm expressing an N-term fragment of hn-RNPH1, residues 1-187. I wanted to know if your antibody (sc-32310) can attach this fragment.

Asked by: Naomita613
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

hello. i purified recombinant hnRNP-H, and reacted it with your antibody. but i always see 3 bends (~55kDa, ~40kDa, ~25kDa). i wanted to ask what is the epitope of the antibody, it may help me to understand the results. thank you Naomi

Asked by: Naomita613
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-03-03

I am using hnRNP F/H (1G11): sc-32310 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBに使用WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of 5 by from Excellent Antibody!I received the free sample, and used for WB on DU145 lysate. I obtained a very satisfatory result, the bands were very strong, specific ! Love it so much :) Highly recommend to you!
Date published: 2018-02-02
Rated 4 out of 5 by from Good for WBit works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Wonderful detection by WBThis antibody works in WB using human MSC lysate from different conditions.It detects both F/H isoforms clearly.
Date published: 2017-05-17
Rated 5 out of 5 by from Published data for WB in human epidermoidPublished data for WB in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Robust signal in WB in Jurkat and KNRK nuclearRobust signal in WB in Jurkat and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Published data for WB in rat PC12 cellsPublished data for WB in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-28
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hnRNP F/H 抗体 (1G11) is rated 4.9 out of 5 by 8.
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