Date published: 2025-9-6

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hnRNP E2 抗体 (23-G): sc-101136

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说明书
  • hnRNP E2抗体(23-G)是小鼠单克隆IgG2a κ, 在12篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组hnRNP E2
  • 推荐用于 mouse, rat 和 human 来源的hnRNP E2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取hnRNP E2 (23-G): sc-101136免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 hnRNP E2 抗体 (23-G) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP E2 抗体 (23-G) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

hnRNP E2抗体(23-G)是一种IgG2a κ小鼠单克隆hnRNP E2抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源hnRNP E2。hnRNP E2抗体(23-G)可作为非偶联抗hnRNP E2抗体形式提供。核糖核蛋白异质体(hnRNP)是一组多肽,它们参与mRNA转录、mRNA前体加工以及成熟mRNA向细胞质的转运和翻译。它们还与核糖核蛋白异质体RNA(hnRNA)结合,hnRNA是由RNA聚合酶II产生的转录本。已知有大约20种hnRNP蛋白质,它们的复合物是剪接体的主要成分。大多数hnRNP蛋白质成分定位于细胞核;然而,一些在细胞核和细胞质之间穿梭,如hnRNP E1和E2。hnRNP E1可能在细胞质中作为翻译调节蛋白发挥作用,而hnRNP E2稳定mRNA以增强脊髓灰质炎病毒mRNA翻译。hnRNP M参与mRNA前体剪接和应激诱导的瞬时剪接阻滞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP E2 抗体 (23-G) 参考文献:

  1. 核前 mRNA 区隔:将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  3. 小鼠胚胎发育过程中 hnRNP M 和 2H9 基因表达的时空调控。  |  Mahé, D., et al. 2000. Biochim Biophys Acta. 1492: 414-24. PMID: 11004512
  4. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  5. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  6. 人类 hnRNP-M 蛋白:结构及与早期热休克诱导的剪接停滞和染色体图谱的关系。  |  Gattoni, R., et al. 1996. Nucleic Acids Res. 24: 2535-42. PMID: 8692693
  7. 红细胞分化过程中的 mRNA 沉默:hnRNP K 和 hnRNP E1 从 3' 端调控 15 脂氧合酶的翻译。  |  Ostareck, DH., et al. 1997. Cell. 89: 597-606. PMID: 9160751

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP E2 抗体 (23-G)

sc-101136
100 µg/ml
$333.00

hnRNP E2 (23-G): m-IgG Fc BP-HRP 套装

sc-539733
100 µg Ab; 10 µg BP
$354.00

hnRNP E2 (23-G): m-IgG2a BP-HRP 套装

sc-546775
100 µg Ab; 10 µg BP
$354.00

why does your PCBP2 western blot result have 2 bands? is the bottom band non-specific band?

Asked by: hrchang
Thank you for your question.The bands are most likely due to some post-translational modifications. pH of the gel buffers and/or running buffers can also sometimes cause slight doublets on Western blots.
Answered by: Tech Service
Date published: 2020-01-14

Can sc-101136: hnRNP E2 (23-G) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-101136: hnRNP E2 (23-G) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from WBにて使用しました。WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of 5 by from hnRNA E2 cellular locationnucleolar location of hnRNPE2, very good to stain.
Date published: 2018-02-19
Rated 5 out of 5 by from good bandIt gives strong signal bands in mouse brain, an intense band of expected size can be seperated,1:500 diluted.
Date published: 2017-08-26
Rated 5 out of 5 by from western blot analysisDetection of hnRNP E2 expression in K562 cells by hnRNP this antibody,we got specific band at 40kda,I suggest use this sample as a positive control.
Date published: 2017-07-15
Rated 5 out of 5 by from Well characterized hnRNP ab for WB, IF & IHCThis is a solid choice for the detection of hnRNP when using WB, IF or IHC(P) There are several citations in the primary literature supporting its use as well as strong WB band in K-562 whole cell lysate.
Date published: 2017-01-18
Rated 4 out of 5 by from Used in WB in human samplesUsed in WB in human samples, worked fine.
Date published: 2015-07-17
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclearProduced robust immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, works perfect for K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-21
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hnRNP E2抗体 (23-G) is rated 4.6 out of 5 by 10.
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