Date published: 2025-9-6
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hnRNP C1/C2 Antibody (4F4) - Western Blotting - Image 378870
hnRNP C1/C2 抗体 (4F4): sc-32308. HeLa (A), Jurkat (B), THP-1 (C), A-431 (D), K-562 (E) 和 MEG-01 (F) 全细胞裂解液中 hnRNP C1/C2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
hnRNP C1/C2 抗体 (4F4): sc-32308. HeLa (A), Jurkat (B), THP-1 (C), A-431 (D), K-562 (E) 和 MEG-01 (F) 全细胞裂解液中 hnRNP C1/C2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

hnRNP C1/C2 抗体 (4F4): sc-32308

4.4(13)
寫評論提問

说明书
  • hnRNP C1/C2 抗体 4F4 是小鼠单克隆 IgG1 κ，hnRNP C1/C2抗体, 在83篇文献中引用，规格为200 µg/ml
  • 针对从寡核苷酸（dT）纤维素柱中洗脱的RNPs
  • hnRNP C1/C2 抗体 (4F4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 hnRNP C1 and hnRNP C2
  • 抗hnRNP C1/C2抗体（4F4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP C1/C2 (4F4): sc-32308的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 hnRNP C1/C2 抗体 (4F4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP C1/C2 抗体 (4F4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 hnRNP 抗体 包括 hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2和hnRNP F

hnRNP C1/C2抗体（4F4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的hnRNP C1/C2蛋白。抗hnRNP C1/C2抗体（4F4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。异质核糖核蛋白（hnRNPs）在预mRNA处理和运输中起着至关重要的作用，与RNA聚合酶II产生的异质核RNA（hnRNA）结合。hnRNP C1和C2蛋白通过与剪接因子相互作用并影响剪接位点选择，对预mRNA剪接调节至关重要。hnRNP C1和C2含有RNA 结合域，有助于这些蛋白质在主要存在的细胞核中发挥作用，尽管一些hnRNP蛋白可以在细胞核和细胞质之间穿梭。hnRNP C1和C2与其他蛋白质（如hnRNP A1和A2/B1）的相互作用对于前mRNA处理调节至关重要，因为这些蛋白质可以对抗剪接因子并影响整体剪接机制。翻译后修饰，包括磷酸化与甲基化，可进一步影响hnRNP蛋白的活性与相互作用，使这些蛋白成为基因表达调控的关键参与者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP C1/C2 抗体 (4F4) 参考文献:

  1. 核前 mRNA 区隔：将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 有丝分裂过程中 hnRNP C1/C2 和 Unr 在内部启动翻译中的作用  |  Schepens, B., et al. 2007. EMBO J. 26: 158-69. PMID: 17159903
  3. hnRNP C1/C2 和 Pur-beta 蛋白介导与端粒悬空同源的寡核苷酸诱导衰老。  |  Mulnix, RE., et al. 2013. Onco Targets Ther. 7: 23-32. PMID: 24379680
  4. 鉴定作为白塞氏病患者自身抗原的 hnRNP C1/C2。  |  Hussain, M., et al. 2018. Iran J Immunol. 15: 133-141. PMID: 29947342
  5. 人核核糖核蛋白颗粒 C 蛋白的一级结构：异质核 RNA, mRNA 和前 rRNA 结合蛋白的序列和结构域的保护。  |  Swanson, MS., et al. 1987. Mol Cell Biol. 7: 1731-9. PMID: 3110598
  6. Ron 第 11 号外显子前 mRNA 的剪接调节需要 hnRNP C1/C2 的 RRM 而非 Asp/Glu 结构域。  |  Moon, H., et al. 2019. BMB Rep. 52: 641-646. PMID: 31401978
  7. 培养少突胶质细胞中 hnRNP C1/C2 相互作用网络结构一瞥。  |  Fioramonte, M., et al. 2021. Biochim Biophys Acta Proteins Proteom. 1869: 140711. PMID: 34403818
  8. 异质核核糖核蛋白 H, H'和 F 是一个普遍表达的亚家族成员，它们是由不同染色体上的基因编码的相关但不同的蛋白质。  |  Honoré, B., et al. 1995. J Biol Chem. 270: 28780-9. PMID: 7499401
  9. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  10. 异质核糖核蛋白 C 蛋白 RNA 结合特异性的决定因素。  |  Görlach, M., et al. 1994. J Biol Chem. 269: 23074-8. PMID: 8083209
  11. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  12. 颉颃物前核糖核酸剪接因子的组织特异性调控表达。  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP C1/C2 抗体 (4F4)

sc-32308
200 µg/ml
RMB2377.00

hnRNP C1/C2 (4F4): m-IgG Fc BP-HRP 套装

sc-528457
200 µg Ab; 10 µg BP
RMB2662.00

hnRNP C1/C2 (4F4): m-IgGκ BP-HRP 套装

sc-520838
200 µg Ab, 40 µg BP
RMB2662.00

hnRNP C1/C2 抗体 (4F4) AC

sc-32308 AC
500 µg/ml, 25% agarose
RMB3129.00

hnRNP C1/C2 抗体 (4F4) HRP

sc-32308 HRP
200 µg/ml
RMB2377.00

hnRNP C1/C2 抗体 (4F4) FITC

sc-32308 FITC
200 µg/ml
RMB2482.00

hnRNP C1/C2 抗体 (4F4) PE

sc-32308 PE
200 µg/ml
RMB2580.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 488

sc-32308 AF488
200 µg/ml
RMB2685.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 546

sc-32308 AF546
200 µg/ml
RMB2685.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 594

sc-32308 AF594
200 µg/ml
RMB2685.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 647

sc-32308 AF647
200 µg/ml
RMB2685.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 680

sc-32308 AF680
200 µg/ml
RMB2685.00

hnRNP C1/C2 抗体 (4F4) Alexa Fluor® 790

sc-32308 AF790
200 µg/ml
RMB2685.00

这个抗体检测的序列是哪一段呢？hnRNPC1还是C2？

Asked by: lihonying
感谢您的提问。 hnRNP C1/C2 Antibody (4F4) 推荐用WB, IP, IF 和 IHC(P)检测小鼠、大鼠和人源的hnRNP C1 和 hnRNP C2。 Thank you for your question. hnRNP C1/C2 Antibody (4F4) is recommended for detection of hnRNP C1 and hnRNP C2 of mouse, rat and human origin by WB, IP, IF and IHC(P).
Answered by: Technical Service SH
Date published: 2023-09-01

how many uL is provided in a purchase of #sc40093?

Asked by: Mahdi Assari
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

Can I use hnRNP C1/C2: sc-32308 for RIP assay of cultured rat cells as in PMID: # 28264987 ?

Asked by: kiwai
Thank you for your question. We have not tested this antibody for RIP assay. However, this publication does indicate that this specific antibody was successfully used for IP, RIP and Western blot.
Answered by: Technical Support
Date published: 2018-12-17

Can I use hnRNP C1/C2 (4F4): sc-32308 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. AQP1 (B-11): sc-25287 monoclonal antibody is available directly conjugated to either HRP (sc-32308 HRP), phycoerythrin (sc-32308 PE), fluorescein (sc-32308 FITC), Alexa Fluor® 488 (sc-32308 AF488) or Alexa Fluor® 647 (sc-32308 AF647).
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from excellent in WBWB in cell lysate of MDA dilution 1:1000 Clear and well detection of a band at the correct molecular weight
Date published: 2019-04-24
Rated 2 out of 5 by from can not be detectedcan not be detected by wetern blot.quite disappointing.
Date published: 2017-10-10
Rated 5 out of 5 by from user commentI used this antibody to do immunofluorescence at 1:500 the previous month,colon cancer cells.Fluorescence intensity is very strong.This antibody is great.
Date published: 2017-08-14
Rated 5 out of 5 by from 非常好用的抗体该抗体功能较多，很实用，做过IP，很好拉相互作用蛋白，200ul体积的包装基本整个实验都够了。表达很好，特异性较好，使用时wb抗体浓度可以1:1000就能有较好的条带
Date published: 2017-05-09
Rated 5 out of 5 by from Great for western blot and IPUsed at 1:400 for Western blot and yielded nice, specific staining. Successfully IPed the endogenous protein at 0.4ug antibody per 200ug lysate.
Date published: 2017-02-11
Rated 5 out of 5 by from Strong WB and IHCGreat antibody; strong western blot and IHC staining data
Date published: 2017-01-11
Rated 5 out of 5 by from Very Strong Signal in WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background.
Date published: 2017-01-04
Rated 4 out of 5 by from Produced wonderful immunoperoxidase nuclearProduced wonderful immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-10
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hnRNP C1/C2 抗体 (4F4) is rated 4.4 out of 5 by 13.
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