Date published: 2025-9-6
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hnRNP A2/B1 Antibody (EF-67) - Western Blotting - Image 356735
hnRNP A2/B1 抗体 (EF-67): sc-53531. A549 (A), MEG-01 (B) 和 NIH/3T3 (C) 全细胞裂解液以及 HL-60 (D), MCF7 (E) 和 K-562 (F) 核提取物中 hnRNP A2/B1 表达的 Western 印迹分析.
hnRNP A2/B1 抗体 (EF-67): sc-53531. A549 (A), MEG-01 (B) 和 NIH/3T3 (C) 全细胞裂解液以及 HL-60 (D), MCF7 (E) 和 K-562 (F) 核提取物中 hnRNP A2/B1 表达的 Western 印迹分析.

hnRNP A2/B1 抗体 (EF-67): sc-53531

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寫評論提問

说明书
  • hnRNP A2/B1抗体(EF-67)是小鼠单克隆IgG1 κ, 在38篇文献中引用，规格为200 µg/ml
  • 针对hnRNP A2的human起源的C-terminus的
  • 抗-hnRNP A2/B1 抗体 (EF-67) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 hnRNP A2/B1; 不会与 hnRNP A1产生交联反应
  • 抗hnRNP A2/B1抗体（EF-67）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP A2/B1 (EF-67): sc-53531的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 hnRNP A2/B1 抗体 (EF-67) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP A2/B1 抗体 (EF-67) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 hnRNP 抗体包括 hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2 和 hnRNP F

hnRNP A2/B1 抗体（EF-67）是一种小鼠单克隆 IgG1 kappa 轻链抗体，它能通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组化 (IHCP) 等应用检测小鼠、大鼠和人样本中的 hnRNP A2/B1。抗 hnRNP A2/B1 抗体 (EF-67) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。hnRNP A2/B1 主要位于细胞核中，与前核糖核酸和剪接因子的相互作用影响剪接位点的选择，确保 mRNA 转录本的正常成熟。hnRNP A2/B1 的核-胞质穿梭可根据细胞信号对基因表达进行动态调控。hnRNP 的 A/B 亚家族（包括 hnRNP A2/B1）对于剪接体复合物的形成至关重要，因此这些蛋白对于准确处理前 mRNA 至关重要。了解 hnRNP A2/B1 的功能和定位有助于阐明基因调控机制以及对癌症和神经退行性疾病的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP A2/B1 抗体 (EF-67) 参考文献:

  1. 核前 mRNA 区隔：将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  3. hnRNP A2/B1与单链脊椎动物端粒重复序列TTAGGGn特异性结合。  |  McKay, SJ. and Cooke, H. 1992. Nucleic Acids Res. 20: 6461-4. PMID: 1282701
  4. 小气道上皮细胞中hnRNP A2/B1蛋白的表达。  |  Satoh, H., et al. 2004. Int J Mol Med. 14: 605-8. PMID: 15375589
  5. hnRNP A2/B1可调节肺癌细胞系中的上皮-间质转化。  |  Tauler, J., et al. 2010. Cancer Res. 70: 7137-47. PMID: 20807810
  6. hnRNP A2/B1与甲型流感病毒蛋白NS1相互作用，可能通过抑制NS1 RNA/蛋白水平和NS1 mRNA核出口来抑制病毒复制。  |  Wang, Y., et al. 2014. Virology. 449: 53-61. PMID: 24418537
  7. 西洛他唑抑制人真皮微血管内皮细胞中hnRNP A2/B1和细胞因子的表达。  |  An, Y., et al. 2017. Clin Exp Rheumatol. 35 Suppl 108: 60-66. PMID: 28850024
  8. 一种新型低丰度 hnRNP 蛋白的分离与鉴定：A0.  |  Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
  9. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  10. 章鱼 RNP 蛋白家族的三个新成员。  |  Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
  11. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  12. 颉颃物前核糖核酸剪接因子的组织特异性调控表达。  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP A2/B1 抗体 (EF-67)

sc-53531
200 µg/ml
RMB2377.00

hnRNP A2/B1 (EF-67): m-IgG Fc BP-HRP 套装

sc-526823
200 µg Ab; 10 µg BP
RMB2662.00

hnRNP A2/B1 (EF-67): m-IgG1 BP-HRP 套装

sc-532196
200 µg Ab; 20 µg BP
RMB2662.00

hnRNP A2/B1 抗体 (EF-67) AC

sc-53531 AC
500 µg/ml, 25% agarose
RMB3129.00

hnRNP A2/B1 抗体 (EF-67) HRP

sc-53531 HRP
200 µg/ml
RMB2377.00

hnRNP A2/B1 抗体 (EF-67) FITC

sc-53531 FITC
200 µg/ml
RMB2482.00

hnRNP A2/B1 抗体 (EF-67) PE

sc-53531 PE
200 µg/ml
RMB2580.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 488

sc-53531 AF488
200 µg/ml
RMB2685.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 546

sc-53531 AF546
200 µg/ml
RMB2685.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 594

sc-53531 AF594
200 µg/ml
RMB2685.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 647

sc-53531 AF647
200 µg/ml
RMB2685.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 680

sc-53531 AF680
200 µg/ml
RMB2685.00

hnRNP A2/B1 抗体 (EF-67) Alexa Fluor® 790

sc-53531 AF790
200 µg/ml
RMB2685.00

你好，抗体后面括号里的内容代表什么意思

Asked by: Alinna
感谢您的提问。括号中的内容是克隆号，没有实际意义。谢谢。 Thank you for your question. The brackets are clone numbers and have no real meaning. Thank you.
Answered by: Jiajun Z
Date published: 2024-03-18

Is this antibody cross reactive with hnRNP A1?

Asked by: AbPolly
Thank you for your question. No, our hnRNP A2/B1 sc-53531 is not cross-reactive with hnRNP A1.
Answered by: Technical Support 9
Date published: 2017-01-11

What fixation methods can be used with this antibody in IF or IHC?

Asked by: AbPolly
Thank you for your question. This antibody has been validated with multiple fixation methods including formalin fixed, paraffin-embedded sections. For complete protocol information, please contact our Technical Service Department or refer to the protocols available online: https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from It works for our murine samplesWe got good signals from this antibody. Our samples derived from murine tumor.
Date published: 2018-02-06
Rated 5 out of 5 by from Great productStrong, clear western data; also great for IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Great antibody!The hnRNP A2/B1 Antibody (EF-67): sc-53531 provides excellent IHC data when working with formalin fixed, paraffin-embedded tissue sections.
Date published: 2017-01-16
Rated 5 out of 5 by from Published data for WB in human brain samplePublished data for WB in human brain sample. -SCBT Publication Review
Date published: 2015-05-13
Rated 5 out of 5 by from Published data for WB in human HuhPublished data for WB in human Huh-7/3-1 cells. -SCBT Publication Review
Date published: 2015-03-20
Rated 5 out of 5 by from Published data for WB in human normal renalPublished data for WB in human normal renal and tumor samples.
Date published: 2015-03-19
Rated 5 out of 5 by from Superb immunoperoxidase nuclear stainingSuperb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Good band in WB in KGood band in WB in K-562, HeLa, MEG-01 and Jurkat nuclear extracts and THP-1 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-07
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hnRNP A2/B1 抗体 (EF-67) is rated 4.9 out of 5 by 9.
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