Date published: 2025-9-8
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HNF-3γ Antibody (A-2) - Western Blotting - Image 15616
HNF-3γ (A-2): sc-74424. Hep G2 全细胞裂解液 (A) 和 Hep G2 核提取物 (B) 中 HNF-3γ 表达的 Western 印迹分析.
HNF-3γ (A-2): sc-74424. Hep G2 全细胞裂解液 (A) 和 Hep G2 核提取物 (B) 中 HNF-3γ 表达的 Western 印迹分析.

HNF-3γ 抗体 (A-2): sc-74424

3.8(4)
寫評論提問

说明书
  • HNF-3 gamma 抗体 A-2 是小鼠单克隆 IgG1 κ，HNF-3γ抗体, 在6篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HNF-3γ的氨基酸1-115
  • HNF-3 gamma 抗体 (A-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HNF-3γ
  • 抗HNF-3 gamma抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-74424 X, 200 µg/0.1 ml)
  • 关于如何获取HNF-3γ (A-2): sc-74424的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是HNF-3γ Antibody (A-2) for WB applications. 的首选辅助检测试剂，这些试剂现与HNF-3γ Antibody (A-2) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加HNF抗体，包括HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α和HNF-6

HNF-3γ抗体（A-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HNF-3γ蛋白。HNF-3γ (A-2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。HNF-3γ蛋白在调节肝脏和其他组织中的基因表达方面发挥着至关重要的作用，是肝细胞核因子家族的一部分，该家族包括HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）和HNF-6。这些蛋白质对于代谢过程和器官发育相关基因的转录调控至关重要。具体而言，HNF-3γ与其他转录因子（如HNF-1和HNF-4）相互作用，协调对肝功能和发育至关重要的基因的表达。HNF-3γ的正常功能至关重要，因为突变或失调会导致代谢紊乱和发育异常。了解HNF-3γ的相互作用和调节机制对于阐明其在健康和疾病中的作用非常重要，这使得抗HNF-3γ抗体（A-2）成为研究人员研究这些过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-3γ 抗体 (A-2) 参考文献:

  1. 腺病毒转导的 HNF-3gamma 基因在 CCl(4) 引起的急性肝损伤中的保肝作用  |  Nakamura, T., et al. 1999. FEBS Lett. 459: 1-4. PMID: 10508906
  2. Foxa3（HNF-3-gamma）能结合并激活大鼠胰高血糖素基因启动子，但对胰高血糖素基因的表达并不重要。  |  Liu, Y., et al. 2002. Biochem J. 366: 633-41. PMID: 12000309
  3. 肝细胞核因子在男性特异性 CYP2A2 转录调控中的作用  |  Wiwi, CA. and Waxman, DJ. 2005. J Biol Chem. 280: 3259-68. PMID: 15539409
  4. 生长激素调节牛肝中肝细胞核因子-3 γ和其他富含肝脏的转录因子的表达。  |  Eleswarapu, S. and Jiang, H. 2005. J Endocrinol. 184: 95-105. PMID: 15642787
  5. 内质网压力下的 FOXA3 诱导是导致非酒精性脂肪肝的原因之一。  |  Liu, C., et al. 2021. J Hepatol. 75: 150-162. PMID: 33548387
  6. 通过 CRISPRa 激活内源性 Gata4 和 Foxa3，将成纤维细胞直接重编程为功能性肝细胞。  |  Li, J., et al. 2024. Chin Med J (Engl). 137: 1351-1359. PMID: 38721807
  7. FOXA3 调节胆固醇代谢，以补偿肺腺癌发展过程中的低吸收率。  |  Wang, D., et al. 2024. PLoS Biol. 22: e3002621. PMID: 38805565
  8. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  9. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  10. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  11. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657
  12. 腺病毒转移的 HNF-3 γ 能保护成年大鼠原代培养肝细胞的某些肝功能。  |  Nakamura, T., et al. 1998. Biochem Biophys Res Commun. 253: 352-7. PMID: 9878541

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-3γ 抗体 (A-2)

sc-74424
200 µg/ml
RMB2377.00

HNF-3γ (A-2): m-IgG Fc BP-HRP 套装

sc-527019
200 µg Ab; 10 µg BP
RMB2662.00

HNF-3γ (A-2): m-IgG1 BP-HRP 套装

sc-532392
200 µg Ab; 20 µg BP
RMB2662.00

HNF-3γ 抗体 (A-2) X

sc-74424 X
200 µg/0.1 ml
RMB2377.00

HNF-3γ 抗体 (A-2) AC

sc-74424 AC
500 µg/ml, 25% agarose
RMB3129.00

HNF-3γ 抗体 (A-2) HRP

sc-74424 HRP
200 µg/ml
RMB2377.00

HNF-3γ 抗体 (A-2) FITC

sc-74424 FITC
200 µg/ml
RMB2482.00

HNF-3γ 抗体 (A-2) PE

sc-74424 PE
200 µg/ml
RMB2580.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 488

sc-74424 AF488
200 µg/ml
RMB2685.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 546

sc-74424 AF546
200 µg/ml
RMB2685.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 594

sc-74424 AF594
200 µg/ml
RMB2685.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 647

sc-74424 AF647
200 µg/ml
RMB2685.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 680

sc-74424 AF680
200 µg/ml
RMB2685.00

HNF-3γ 抗体 (A-2) Alexa Fluor® 790

sc-74424 AF790
200 µg/ml
RMB2685.00

What’s the concentration of protein Lysate loaded for the hepg2 wb and the dilution of primary ab used? Thanks

Asked by: Kp233
Thank you for the question. We suggest to follow our WB protocol https://www.scbt.com/scbt/de/resources/protocols/western-immuno-blotting and prior to optimization start with the antibody concentration at 1:200 and load 50µg protein HepG2 lysate.
Answered by: Technical Support Europe
Date published: 2018-02-05

When should the TransCruz reagent sc-74424 X be used instead of the standard form sc-74424?

Asked by: Randy McDonald
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Quite good antibodyThe antibody works, with a good band detected at the predicted molecular weight - with RIPA lysis buffer. Though, some aspecific signals are detected as well.
Date published: 2025-05-19
Rated 1 out of 5 by from HNF3g antibody does not workAfter postive controls were included, we concluded that this antibody did not work with mouse or human HNF3g. You may see bands around ~ 50 kDa, but they are not true HNF3g bands.
Date published: 2022-08-18
Rated 5 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Specific and strong band detected in HepSpecific and strong band detected in Hep G2 whole cell lysate and Hep G2 nuclear extract. - SCBT QC
Date published: 2015-02-19
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HNF-3γ 抗体 (A-2) is rated 3.8 out of 5 by 4.
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