Date published: 2025-9-7
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HNF-3α Antibody (Q-6) - Western Blotting - Image 70521
HNF-3α (Q-6): sc-101058. 未转染: sc-117752 (A) 和人 HNF-3α 转染: sc-128813 (B) 293T 全细胞裂解液和 HeLa 核提取物 (C) 中 HNF-3α 表达的 Western 印迹分析.
HNF-3α Antibody (Q-6) - Western Blotting - Image 70521
HNF-3α (Q-6): sc-101058. 未转染: sc-117752 (A) 和人 HNF-3α 转染: sc-128813 (B) 293T 全细胞裂解液和 HeLa 核提取物 (C) 中 HNF-3α 表达的 Western 印迹分析.
HNF-3α Antibody (Q-6) - Immunohistochemistry - Image 36274
HNF-3α (Q-6): sc-101058. 福尔马林固定, 石蜡包埋的人前列腺组织的免疫过氧化物酶染色显示核定位.
HNF-3α Antibody (Q-6) - Western Blotting - Image 34425
HNF-3a (Q-6): sc-101058. Hep G2 全细胞裂解液中 HNF-3a 表达的 Western 印迹分析.
HNF-3α (Q-6): sc-101058. 未转染: sc-117752 (A) 和人 HNF-3α 转染: sc-128813 (B) 293T 全细胞裂解液和 HeLa 核提取物 (C) 中 HNF-3α 表达的 Western 印迹分析.

HNF-3α 抗体 (Q-6): sc-101058

4.4(7)
寫評論提問

说明书
  • HNF-3α抗体(Q-6)是小鼠单克隆IgG2a κ, 在34篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组HNF-3α
  • 推荐用于 mouse, rat 和 human 来源的HNF-3α WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 HNF-3α (A-3): sc-514695，可查看有标记的 HNF-3α 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取HNF-3α (Q-6): sc-101058的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 HNF-3α 抗体 (Q-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HNF-3α 抗体 (Q-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HNF 抗体包括 HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α 和 HNF-6

HNF-3α 抗体（Q-6）是一种 IgG2a κ 小鼠单克隆 HNF-3α 抗体（也称为 HNF-3α 抗体或 FOXA1 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类来源的 HNF-3α。HNF-3α 抗体（Q-6）为非偶联的抗 HNF-3α 抗体形式。HNF-1（α 和 β）、HNF-3（α、β 和 γ）、HNF-4（α 和 γ）以及 HNF-6 部分组成了一个称为肝细胞核因子家族的同源蛋白家族。各种 HNF-1 亚型在肝脏以及其他组织（如肾脏、小肠和胸腺）中调节基因的转录。HNF-3α、HNF-3β 和 HNF-3γ 在成年肝脏中调节众多肝细胞基因的转录。HNF-3α 和 HNF-3β 还参与原肠胚形成事件，如体轴形成。HNF-4α 和 HNF-4γ 对于早期胚胎发育很重要。HNF-4α 在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；HNF-4γ 在除肝脏外的这些组织中均有表达。已显示 HNF-6 可与 HNF-3β 的启动子结合，这表明 HNF-6 在肠内胚层上皮细胞分化中的潜在作用。有证据表明，HNF-6 也可能是至少 22 个其他肝细胞富集基因的转录激活因子，包括细胞色素 P450 2C13 和 α-1 抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-3α 抗体 (Q-6) 参考文献:

  1. 肝核因子-3（HNF-3 或 Foxa2）通过与 G1 和 G2 启动子元件结合来调节胰高血糖素基因的转录。  |  Gauthier, BR., et al. 2002. Mol Endocrinol. 16: 170-83. PMID: 11773447
  2. 腺病毒介导的 HNF-3beta 或 HNF-3alpha 的增加在肝糖原水平和基因表达方面显示出差异。  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
  3. HNF-3beta是Jaagsiekte绵羊逆转录病毒长末端重复序列在II型肺细胞中表达的关键因素，而不是在克拉拉细胞中表达的关键因素。  |  McGee-Estrada, K., et al. 2002. Virology. 292: 87-97. PMID: 11878911
  4. 肝细胞中叉头盒 A2 (HNF-3beta) 转录因子水平升高会导致出生后脂肪变性和线粒体损伤。  |  Hughes, DE., et al. 2003. Hepatology. 37: 1414-24. PMID: 12774021
  5. 转录因子HNF-6/OC-1可抑制TGF-beta对小鼠肝脏中HNF-3α/Foxa1基因的刺激。  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
  6. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  7. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  8. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  9. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-3α 抗体 (Q-6)

sc-101058
100 µg/ml
RMB2504.00

HNF-3α (Q-6): m-IgG Fc BP-HRP 套装

sc-537297
100 µg Ab; 10 µg BP
RMB2662.00

HNF-3α (Q-6): m-IgGκ BP-HRP 套装

sc-534529
100 µg Ab; 40 µg BP
RMB2662.00

HNF-3α (Q-6): m-IgG2a BP-HRP 套装

sc-547967
100 µg Ab; 10 µg BP
RMB2662.00

What is the recommended secondary reagent for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 10
Date published: 2017-01-16

I would like to use HNF-3α (Q-6): sc-101058 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101058 is not available directly conjugated, but we have an alternative HNF-3α antibody (sc-514695) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2017-01-16
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Rated 5 out of 5 by from GOOD FOR WBgood signal for FOXA1 (HNF3a) in WB on A549 with silencing validation
Date published: 2025-06-11
Rated 4 out of 5 by from Good productIt is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
Rated 5 out of 5 by from gute Western Blot ErgebnisseTarget ist mit HNF3a-Antikörper ist sehr gut und sauber nachweisbar Ansatz mit 1:250 Verdünnung hat gut geklappt sehr zufrieden!
Date published: 2017-10-17
Rated 5 out of 5 by from Superb data !!Very strong clean western blot data; also great for IHC
Date published: 2017-01-14
Rated 5 out of 5 by from Specific staining in IHC(P)Great nuclear localization of staining when used in IHC with paraffin-embedded sections.
Date published: 2016-12-22
Rated 4 out of 5 by from Utilized this antibody in IHC and visualizedUtilized this antibody in IHC and visualized good stains
Date published: 2015-03-31
Rated 4 out of 5 by from Works well in IHC(P) with human prostate tissueWorks well in IHC(P) with human prostate tissue -SCBT QC
Date published: 2015-01-18
Rated 4 out of 5 by from Good detection of HNFGood detection of HNF-3 alpha in transfected lysates and nuclear extracts in Western Blot -SCBT QC
Date published: 2013-07-26
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HNF-3α 抗体 (Q-6) is rated 4.5 out of 5 by 8.
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