Date published: 2025-10-23
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HNF-1α Antibody (F-7) - Western Blotting - Image 380689
HNF-1α 抗体 (F-7): sc-393925. Jurkat (A), Hep G2 (B), HUV-EC-C (C), c4 (D), WEHI-231 (E) 和 PC-12 (F) 全细胞裂解液中 HNF-1α 表达的 Western 印迹分析.
HNF-1α 抗体 (F-7): sc-393925. Jurkat (A), Hep G2 (B), HUV-EC-C (C), c4 (D), WEHI-231 (E) 和 PC-12 (F) 全细胞裂解液中 HNF-1α 表达的 Western 印迹分析.

HNF-1α 抗体 (F-7): sc-393925

4.1(8)
寫評論提問

说明书
  • HNF-1 alpha 抗体 F-7 是小鼠单克隆 IgG2a κ，HNF-1α抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HNF-1α的氨基酸80-284
  • HNF-1 alpha 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HNF-1α; 也和以下物种反应，包括： 和 canine and porcine
  • 抗HNF-1 alpha抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-393925 X, 200 µg/0.1 ml)
  • m-IgGκ BP-HRP是HNF-1α Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与HNF-1α Antibody (F-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加HNF抗体，包括HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α和HNF-6

HNF-1α抗体（F-7）是一种IgG2a κ型小鼠单克隆HNF-1α抗体（也称为HNF-1α抗体），能够通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的HNF-1α蛋白。HNF-1α抗体（F-7）既可以是非偶联的抗HNF-1α抗体形式，也可以是多种偶联形式的抗HNF-1α抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）以及HNF-6部分地组成了一个称为肝细胞核因子家族的同源蛋白家族。不同的HNF-1同种型调控肝脏以及其他组织（如肾脏、小肠和胸腺）中的基因转录。HNF-3α、HNF-3β和HNF-3γ调控成年肝脏中众多肝细胞基因的转录。此外，HNF-3α和HNF-3β已被证明参与体轴形成等原肠作用事件。HNF-4α和HNF-4γ对于早期胚胎发育至关重要。HNF-4α在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；而HNF-4γ则在这些组织中表达，除了肝脏。研究表明，HNF-6能够结合到HNF-3β的启动子上，这表明HNF-6在肠道内胚层上皮细胞分化中可能发挥潜在作用。证据表明，HNF-6也可能是至少22种肝细胞富集基因的转录激活剂，包括细胞色素P450 2C13和α-1抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-1α 抗体 (F-7) 参考文献:

  1. HNF-1 alpha 的常见变异与 2 型糖尿病风险。  |  Holmkvist, J., et al. 2006. Diabetologia. 49: 2882-91. PMID: 17033837
  2. 驱动β1,3-半乳糖基转移酶β3Gal-T5在人和小鼠组织中表达的逆转录病毒启动子和原生启动子的比较分析  |  Mare, L. and Trinchera, M. 2007. J Biol Chem. 282: 49-57. PMID: 17107959
  3. 杂合子 HNF-1beta 基因缺失导致的肾囊肿和糖尿病。  |  Eller, P., et al. 2007. Nephrol Dial Transplant. 22: 1271-2. PMID: 17127696
  4. 人体肝脏切片中胆汁酸诱导的 FXR 靶基因调控分析。  |  Jung, D., et al. 2007. Liver Int. 27: 137-44. PMID: 17241392
  5. 肝细胞核因子-1α和-1β的双基因遗传与成熟期发病的年轻糖尿病, 多囊甲状腺和泌尿生殖系统畸形有关。  |  Karges, B., et al. 2007. Diabetes Care. 30: 1613-4. PMID: 17337496
  6. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  7. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  8. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  9. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-1α 抗体 (F-7)

sc-393925
200 µg/ml
RMB2377.00

HNF-1α (F-7): m-IgGκ BP-HRP 套装

sc-551112
200 µg Ab; 40 µg BP
RMB2662.00

HNF-1α 抗体 (F-7) X

sc-393925 X
200 µg/0.1 ml
RMB2377.00

HNF-1α 抗体 (F-7) AC

sc-393925 AC
500 µg/ml, 25% agarose
RMB3129.00

HNF-1α 抗体 (F-7) HRP

sc-393925 HRP
200 µg/ml
RMB2377.00

HNF-1α 抗体 (F-7) FITC

sc-393925 FITC
200 µg/ml
RMB2482.00

HNF-1α 抗体 (F-7) PE

sc-393925 PE
200 µg/ml
RMB2580.00

HNF-1α 抗体 (F-7) Alexa Fluor® 488

sc-393925 AF488
200 µg/ml
RMB2685.00

HNF-1α 抗体 (F-7) Alexa Fluor® 546

sc-393925 AF546
200 µg/ml
RMB2685.00

HNF-1α 抗体 (F-7) Alexa Fluor® 594

sc-393925 AF594
200 µg/ml
RMB2685.00

HNF-1α 抗体 (F-7) Alexa Fluor® 647

sc-393925 AF647
200 µg/ml
RMB2685.00

HNF-1α 抗体 (F-7) Alexa Fluor® 680

sc-393925 AF680
200 µg/ml
RMB2685.00

HNF-1α 抗体 (F-7) Alexa Fluor® 790

sc-393925 AF790
200 µg/ml
RMB2685.00

Can HNF-1α (F-7): sc-393925 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HNF-1α (F-7): sc-393925 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from It works fineIt worked for western blot applicationdiluted at 1:500. However in my cell line (OE19) the antibody detects a stronger band between 45 and 55 KDa.
Date published: 2022-03-23
Rated 5 out of 5 by from 抗体不错本人半月前申请的HNF-1a小样试用装，跑了蛋白，条带不错，推荐使用，另外，有现货的话，基本一天到货，赞
Date published: 2017-09-22
Rated 4 out of 5 by from GoodThe antibody works well, especially after overnight incubation. I used at 1:1000 dilution.The band is strong and clear,the background is also clear.
Date published: 2017-07-14
Rated 5 out of 5 by from Great has been the antibody, than we have to buy tSalesman to apply for HBF1a antibodies, used, and very good use of this protein before the Abcam has not been used to do the experiment, although it is a trial installation, but better than before.
Date published: 2017-01-07
Rated 1 out of 5 by from Unsuitable for IHC(P)Unsuitable for IHC(P). Unspecifically stains confirmed HNF1A negative samples at various dilutions, no difference between HNF1A positive and negative samples. Cytoplasmic instead of nuclear staining. Not an alternative for sc-8986 for this application.
Date published: 2016-08-16
Rated 5 out of 5 by from I usedI used, it is good to use, than before to buy some of the antibodies are actually better use
Date published: 2016-08-02
Rated 5 out of 5 by from Very good quality on Rat Liver tissue in WBVery good quality on Rat Liver tissue in WB.we tried 1:500 to 1:100. The optimize dilution is 1:300. I use lot:B0514. I give full score of this antibody. I'll order again.
Date published: 2015-09-09
Rated 5 out of 5 by from Great results in Western Blot with Hep G2Great results in Western Blot with Hep G2 whole cell lysate and human liver and rat liver tissue extracts -SCBT QC
Date published: 2014-02-15
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HNF-1α 抗体 (F-7) is rated 4.1 out of 5 by 8.
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