Date published: 2025-9-7
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HMG-I/HMG-Y Antibody (D-12) - Immunohistochemistry - Image 375230
HMG-I/HMG-Y 抗体 (D-12): sc-393213. 福尔马林固定, 石蜡包埋的人上胃组织的免疫过氧化物酶染色, 显示腺细胞的核和胞浆染色.
HMG-I/HMG-Y 抗体 (D-12): sc-393213. 福尔马林固定, 石蜡包埋的人上胃组织的免疫过氧化物酶染色, 显示腺细胞的核和胞浆染色.

HMG-I/HMG-Y 抗体 (D-12): sc-393213

4.7(9)
寫評論提問

说明书
  • HMG-I/HMG-Y 抗体 D-12 是小鼠单克隆 IgG2b κ，HMG-I/HMG-Y抗体, 在8篇文献中引用，规格为200 µg/ml
  • 与氨基酸反应 1-95 代表human起源的全长HMG Y
  • HMG-I/HMG-Y 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HMG-l and HMG-Y
  • 抗HMG-I/HMG-Y抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-393213 X, 200 µg/0.1 ml)
  • 关于如何获取HMG-I/HMG-Y (D-12): sc-393213的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 HMG-I/HMG-Y 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HMG-I/HMG-Y 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HMG 抗体包括 HMG-1, HMG-14, HMG-2, HMG-20A, HMG-2L1, HMG-I, HMG-Y 和 HMGI-C

HMG-I/HMG-Y抗体（D-12）是一种IgG2b κ小鼠单克隆HMG-I/HMG-Y抗体（也被指定为HMGA1抗体，或HMGIY抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组化法（IHC(P)）和酶联免疫吸附测定法（ELISA）检测来自小鼠、大鼠和人类的HMG-I/HMG-Y蛋白质。HMG-I/HMG-Y抗体（D-12）既有非偶联抗HMG-I/HMG-Y抗体形式，也有多种偶联形式的抗HMG-I/HMG-Y抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。高迁移率族（HMG）I（Y）染色质蛋白与富含AT的DNA序列的小沟具有高亲和力。有证据表明，HMG蛋白与DNA的结合会诱导DNA结构发生变化，包括DNA弯曲和解旋。HMG蛋白与Oct-2、NFkB家族成员、ATF-2和c-Jun协同作用以激活转录。其他研究表明，HMG蛋白的磷酸化是刺激该蛋白转录活性所必需的。人类HMG I（Y）含有两个DNA结合域，称为HMG盒。HMG蛋白结合单链DNA，但仅诱导双链DNA的构象变化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HMG-I/HMG-Y 抗体 (D-12) 参考文献:

  1. HMG I(Y) 在 IFN-beta 增强体的组装和功能中的作用。  |  Yie, J., et al. 1999. EMBO J. 18: 3074-89. PMID: 10357819
  2. HMG-I/Y，一种新的 c-Myc 目的基因和潜在的癌基因。  |  Wood, LJ., et al. 2000. Mol Cell Biol. 20: 5490-502. PMID: 10891489
  3. HMG I 蛋白：在基因激活, 发育和肿瘤发生中的动态作用。  |  Liu, F., et al. 2001. Immunol Res. 24: 13-29. PMID: 11485207
  4. 人类乳腺癌细胞系中的 HMG-I/Y。  |  Dolde, CE., et al. 2002. Breast Cancer Res Treat. 71: 181-91. PMID: 12002338
  5. 人类 HMG2 蛋白编码基因的结构。  |  Shirakawa, H. and Yoshida, M. 1992. J Biol Chem. 267: 6641-5. PMID: 1551873
  6. HMG 染色体蛋白及其基因的结构特征。  |  Bustin, M., et al. 1990. Biochim Biophys Acta. 1049: 231-43. PMID: 2200521
  7. 编码非组蛋白染色体蛋白 HMG-1 的人类胎盘 cDNA 克隆。  |  Wen, L., et al. 1989. Nucleic Acids Res. 17: 1197-214. PMID: 2922262
  8. 除 NF-kappa B 外，HMGI(Y) 和 Sp1 还调控 MGSA/GRO alpha 基因的转录。  |  Wood, LD., et al. 1995. Nucleic Acids Res. 23: 4210-9. PMID: 7479086
  9. 雷帕霉素可抑制白细胞介素 4 诱导的 HMG-I（Y）磷酸化。  |  Wang, DZ., et al. 1995. J Biol Chem. 270: 22924-32. PMID: 7559428
  10. 建筑转录因子 LEF-1 弯曲 DNA 的结构基础  |  Love, JJ., et al. 1995. Nature. 376: 791-5. PMID: 7651541
  11. 通过与高迁移率基团蛋白 I/Y 结合，封闭的环状 DNA 中出现了超重性变化。  |  Nissen, MS. and Reeves, R. 1995. J Biol Chem. 270: 4355-60. PMID: 7876198
  12. 转录因子和结构蛋白 HMG I(Y)可逆转 IFN beta 基因增强子中固有的 DNA 弯曲。  |  Falvo, JV., et al. 1995. Cell. 83: 1101-11. PMID: 8548798

订购信息

产品名称产品编号规格价格数量收藏夹

HMG-I/HMG-Y 抗体 (D-12)

sc-393213
200 µg/ml
RMB2377.00

HMG-I/HMG-Y (D-12): m-IgG Fc BP-HRP 套装

sc-526227
200 µg Ab; 10 µg BP
RMB2662.00

HMG-I/HMG-Y (D-12): m-IgG2b BP-HRP 套装

sc-549696
200 µg Ab; 10 µg BP
RMB2662.00

HMG-I/HMG-Y 抗体 (D-12) X

sc-393213 X
200 µg/0.1 ml
RMB2377.00

HMG-I/HMG-Y 抗体 (D-12) AC

sc-393213 AC
500 µg/ml, 25% agarose
RMB3129.00

HMG-I/HMG-Y 抗体 (D-12) HRP

sc-393213 HRP
200 µg/ml
RMB2377.00

HMG-I/HMG-Y 抗体 (D-12) FITC

sc-393213 FITC
200 µg/ml
RMB2482.00

HMG-I/HMG-Y 抗体 (D-12) PE

sc-393213 PE
200 µg/ml
RMB2580.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 488

sc-393213 AF488
200 µg/ml
RMB2685.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 546

sc-393213 AF546
200 µg/ml
RMB2685.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 594

sc-393213 AF594
200 µg/ml
RMB2685.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 647

sc-393213 AF647
200 µg/ml
RMB2685.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 680

sc-393213 AF680
200 µg/ml
RMB2685.00

HMG-I/HMG-Y 抗体 (D-12) Alexa Fluor® 790

sc-393213 AF790
200 µg/ml
RMB2685.00

Good afternoon, can this antibody be used as a neutralizing/blocking antibody for an in vivo experiment?

Asked by: Aleflower
Thank you for your question. HMG-I/HMG-Y Antibody (D-12): sc-393213 has only been tested for detection of HMG-l and HMG-Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. We do not have any data on this antibody's ability to block its target protein in vivo.
Answered by: Tech Support Europe
Date published: 2023-03-27

Can this antibody be used to detect HMGA1?

Asked by: Chang Xing
Thank you for your question. HMG-I/HMG-Y Antibody (D-12): sc-393213 has been raised against full length HMG Y of human origin and recognizes HMG-I/HMG-Y, which is the protein that the HMGA1 gene encodes.
Answered by: Tech Support Europe
Date published: 2020-08-04

Can HMG-I/HMG-Y (D-12): sc-393213 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, HMG-I/HMG-Y (D-12): sc-393213 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from ottimo funzionamento!ho comprato questo anticorpo un mese fa e sono molto soddisfatto
Date published: 2023-12-06
Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from HMG-I/HMG-Y is goodWorks well for WB(in 1/1000), the antibody is high in potency and cear distinct bands, I think this antibody is very helpful to the experiment
Date published: 2017-10-18
Rated 5 out of 5 by from GOODI used this antibody to detect MCF-7 WB (1:500), obtained clear bands
Date published: 2017-09-15
Rated 5 out of 5 by from Great antibodyGreat antibody binding HMG-I/HMG-Y for western blotting. used 1:800 dilution for mouse stomach tissue detection.The signal is very strong.
Date published: 2017-08-18
Rated 5 out of 5 by from antibody for wbI used this antibody to detect human breast cancer WB (diluted 1:200), obtained clear bands and strong signals.
Date published: 2016-12-17
Rated 5 out of 5 by from Functional antibody for IHCThese days, I used this antibody for IHC (dilution 1:25) and got very special staining in human cancer. And I am going to use it as followed work!Thank you so much for your support!
Date published: 2016-11-10
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear andExceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-07
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HMG-I/HMG-Y 抗体 (D-12) is rated 4.7 out of 5 by 9.
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