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HLA-G Antibody (4H84) - Western Blotting - Image 6215
HLA-G 抗体 (4H84): sc-21799. Hs 67 全细胞裂解液中 HLA-G 表达的 Western 印迹分析.
HLA-G 抗体 (4H84): sc-21799. Hs 67 全细胞裂解液中 HLA-G 表达的 Western 印迹分析.

HLA-G 抗体 (4H84): sc-21799

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说明书
  • HLA-G 抗体 4H84 是小鼠单克隆 IgG1 κ，HLA-G抗体, 在83篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HLA-G的氨基酸61-83
  • HLA-G 抗体 (4H84) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 HLA-G isoforms
  • 抗HLA-G抗体（4H84）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HLA-G (4H84): sc-21799的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HLA-G 抗体 (4H84) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HLA-G 抗体 (4H84) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HLA 抗体 包括 HLA-A, HLA-A2, HLA-B, HLA-B27, HLA-B7, HLA-C, HLA class I, HLA-D, HLA-DM和HLA-DM α

HLA-G抗体（4H84）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的HLA-G蛋白。抗HLA-G抗体（4H84）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HLA-G在免疫耐受中起着至关重要的作用，尤其是在怀孕期间，它通过抑制母体T淋巴细胞的活化和防止胎儿排斥。这种独特的功能对于维持健康的妊娠至关重要，因为HLA-G允许半同种异体胎儿逃避母体的免疫反应。HLA-G主要在胎盘的滋养层细胞中表达，在那里HLA-G起着保护屏障的作用，确保发育中的胎儿不受母体免疫系统的攻击。HLA-G调节免疫反应的能力归因于它与免疫细胞上的抑制受体（如白细胞免疫球蛋白样受体亚家族B成员1（LILRB1）和杀手免疫球蛋白样受体（KIR）家族）的相互作用，这进一步强调了其在生殖免疫学以及移植医学和癌症免疫治疗中的潜在治疗应用的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HLA-G 抗体 (4H84) 参考文献:

  1. 不同 HLA-G 单克隆抗体对可溶性 HLA-G 分子的反应性比较。  |  Fournel, S., et al. 2000. Tissue Antigens. 55: 510-8. PMID: 10902607
  2. 早期胚胎中 HLA-G 的表达是怀孕的基本前提。  |  Fuzzi, B., et al. 2002. Eur J Immunol. 32: 311-5. PMID: 11807769
  3. 肺巨噬细胞和树突状细胞在肺部疾病中表达 HLA-G 分子。  |  Pangault, C., et al. 2002. Hum Immunol. 63: 83-90. PMID: 11821155
  4. HIV-1 阳性患者的单核细胞和 T 淋巴细胞表达 HLA-G 分子。  |  Lozano, JM., et al. 2002. AIDS. 16: 347-51. PMID: 11834945
  5. 二硫键介导的细胞表面 HLA-G 二聚化。  |  Boyson, JE., et al. 2002. Proc Natl Acad Sci U S A. 99: 16180-5. PMID: 12454284
  6. 识别 HLA-G 或 HLA-E 的单克隆抗体的特征：分析非典型 HLA I 类分子表达的新工具。  |  Menier, C., et al. 2003. Hum Immunol. 64: 315-26. PMID: 12590976
  7. 人类白细胞抗原-G的许多方面：与妊娠结局的相关性。  |  Dahl, M., et al. 2014. J Immunol Res. 2014: 591489. PMID: 24741608
  8. 甲状腺癌患者血浆人类白细胞抗原-G（HLA-G）。  |  Akın, M., et al. 2017. Turk J Med Sci. 47: 1263-1266. PMID: 29156872
  9. 人类白细胞抗原G在胃肠道癌中的预后和临床病理价值：荟萃分析。  |  Peng, Y., et al. 2021. Front Oncol. 11: 642902. PMID: 34055611
  10. 人类白细胞抗原-G（HLA-G）的表达在子宫内膜癌的诊断和分级中起着重要作用。  |  Bai, Y., et al. 2022. J Obstet Gynaecol. 42: 641-647. PMID: 34382498
  11. HLA-G与反复流产。  |  Barbaro, G., et al. 2023. Int J Mol Sci. 24: PMID: 36768880
  12. 人类白细胞抗原（HLA）I类易感等位基因增加了感染率和严重率。  |  Guo, X., et al. 2023. Int J Immunogenet. 50: 163-176. PMID: 37415092

订购信息

产品名称产品编号规格价格数量收藏夹

HLA-G 抗体 (4H84)

sc-21799
200 µg/ml
RMB2377.00

HLA-G (4H84): m-IgG Fc BP-HRP 套装

sc-528372
200 µg Ab; 10 µg BP
RMB2662.00

HLA-G (4H84): m-IgGκ BP-HRP 套装

sc-520734
200 µg Ab, 40 µg BP
RMB2662.00

HLA-G (4H84): m-IgG1 BP-HRP 套装

sc-542900
200 µg Ab; 20 µg BP
RMB2662.00

HLA-G 抗体 (4H84) AC

sc-21799 AC
500 µg/ml, 25% agarose
RMB3129.00

HLA-G 抗体 (4H84) HRP

sc-21799 HRP
200 µg/ml
RMB2377.00

HLA-G 抗体 (4H84) FITC

sc-21799 FITC
200 µg/ml
RMB2482.00

HLA-G 抗体 (4H84) PE

sc-21799 PE
200 µg/ml
RMB2580.00

HLA-G 抗体 (4H84) Alexa Fluor® 488

sc-21799 AF488
200 µg/ml
RMB2685.00

HLA-G 抗体 (4H84) Alexa Fluor® 546

sc-21799 AF546
200 µg/ml
RMB2685.00

HLA-G 抗体 (4H84) Alexa Fluor® 594

sc-21799 AF594
200 µg/ml
RMB2685.00

HLA-G 抗体 (4H84) Alexa Fluor® 647

sc-21799 AF647
200 µg/ml
RMB2685.00

HLA-G 抗体 (4H84) Alexa Fluor® 680

sc-21799 AF680
200 µg/ml
RMB2685.00

HLA-G 抗体 (4H84) Alexa Fluor® 790

sc-21799 AF790
200 µg/ml
RMB2685.00

i'm looking for the best ihc(P) antibody/combo

Asked by: stevo
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

How can i request the free sample antibody to try it on my cell lines

Asked by: Najla
Thank you for your question. Please contact our Technical Service Department directly to arrange for a free 10 µg sample of HLA-G (4H84): sc-21799.
Answered by: Tech Support Europe
Date published: 2021-01-13

I did not succeed to stain placenta with this antibody in automatic immunohistochemistry method with automatic detection kit with DAB IN DILUTION 1:50. could you help me. thanks

Asked by: sit30
Thank you for your question. Please contact your local distributor or technical service directly for assistance regarding your IHC results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-11-19

Can HLA-G Antibody (4H84): sc-21799 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Yes, HLA-G Antibody (4H84): sc-21799 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2021-08-22
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Rated 5 out of 5 by from Great packaging, reliable antibody.Great packaging, reliable antibody. Ordered before!
Date published: 2025-03-15
Rated 5 out of 5 by from Fixed cell, FASCThis antibody was tested on fixed JEG-3 cells by FASC and got a good signal.
Date published: 2023-12-12
Rated 1 out of 5 by from HLA-G western blotI bought this antibody last month for western blot experiment but until now i did not detect the band.
Date published: 2021-02-03
Rated 1 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Good stainingNo problem on human tonsil IHC, demasking pH 9, 1:50 dilution.
Date published: 2017-11-08
Rated 5 out of 5 by from goodI used this antibody sample and i am so glad that i got good resultsof IF.But i used 1:50.
Date published: 2017-09-15
Rated 5 out of 5 by from Excellent antibodyStrong western with low background;also nice ihc results
Date published: 2017-01-03
Rated 5 out of 5 by from A great human reactive antibody.A great choice for staining and blotting human samples. Provides strong bands in WB and clear staining in IHC. It has been cited more than 20 times.
Date published: 2016-12-29
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HLA-G 抗体 (4H84) is rated 4.4 out of 5 by 17.
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