Date published: 2025-9-6
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HLA-DR/DP/DQ/DX Antibody (CR3/43) - Western Blotting - Image 13454
HLA-DR/DP/DQ/DX 抗体 (CR3/43): sc-53302. Raji (A) 和 GA-10 (B) 全细胞裂解液中 HLA-DR/DP/DQ/DX 表达的 Western 印迹分析.
HLA-DR/DP/DQ/DX 抗体 (CR3/43): sc-53302. Raji (A) 和 GA-10 (B) 全细胞裂解液中 HLA-DR/DP/DQ/DX 表达的 Western 印迹分析.

HLA-DR/DP/DQ/DX 抗体 (CR3/43): sc-53302

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寫評論提問

说明书
  • HLA-DR/DP/DQ/DX 抗体 CR3/43 是小鼠单克隆 IgG1 κ，HLA-DR/DP/DQ/DX抗体, 在13篇文献中引用，规格为200 µg/ml
  • 针对human来源的扁桃体细胞
  • HLA-DR/DP/DQ/DX 抗体 (CR3/43) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 HLA-DR/DP/DQ/DX
  • 抗HLA-DR/DP/DQ/DX抗体（CR3/43）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HLA-DR/DP/DQ/DX (CR3/43): sc-53302的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 HLA-DR/DP/DQ/DX 抗体 (CR3/43) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HLA-DR/DP/DQ/DX 抗体 (CR3/43) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
说明书与实验方案
研究信息

関連項目

此外 HLA 抗体包括 HLA-A, HLA-A2, HLA-B, HLA-B27, HLA-B7, HLA-C, HLA class I, HLA-D, HLA-DM 和 HLA-DM α

HLA-DR/DP/DQ/DX抗体（CR3/43）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测HLA-DR、HLA-DP、HLA-DQ和HLA-DX蛋白，统称为人类白细胞抗原II类分子。抗HLA-DR/DP/DQ/DX抗体（CR3/43）针对人类扁桃体细胞产生，确保在各种应用中具有强大的反应性，包括人类样本的蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。HLA-DR/DP/DQ/DX分子在免疫系统中起着至关重要的作用，它们向CD4+ T辅助细胞呈递抗原肽，从而协调适应性免疫反应。这些II类分子与HLA-DM和HLA-DO等关键蛋白相互作用，这些蛋白对于肽在细胞内和溶酶体内装载和交换至关重要。翻译后修饰，特别是糖基化，对于HLA II类蛋白的正确折叠、稳定性和表面表达至关重要，有助于有效的抗原呈递和免疫识别。HLA-DR/DP/DQ/DX 单克隆抗体（CR3/43）有未结合形式和各种结合形式，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，可满足各种实验需求。抗HLA-DR/DP/DQ/DX抗体（CR3/43）有助于推进免疫学研究，并开发旨在操纵MHC II类途径的治疗策略。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

HLA-DR/DP/DQ/DX 抗体 (CR3/43)

sc-53302
200 µg/ml
RMB2377.00

HLA-DR/DP/DQ/DX (CR3/43): m-IgG Fc BP-HRP 套装

sc-526791
200 µg Ab; 10 µg BP
RMB2662.00

HLA-DR/DP/DQ/DX (CR3/43): m-IgG1 BP-HRP 套装

sc-532164
200 µg Ab; 20 µg BP
RMB2662.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) AC

sc-53302 AC
500 µg/ml, 25% agarose
RMB3129.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) HRP

sc-53302 HRP
200 µg/ml
RMB2377.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) FITC

sc-53302 FITC
200 µg/ml
RMB2482.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) PE

sc-53302 PE
200 µg/ml
RMB2580.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 488

sc-53302 AF488
200 µg/ml
RMB2685.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 546

sc-53302 AF546
200 µg/ml
RMB2685.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 594

sc-53302 AF594
200 µg/ml
RMB2685.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 647

sc-53302 AF647
200 µg/ml
RMB2685.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 680

sc-53302 AF680
200 µg/ml
RMB2685.00

HLA-DR/DP/DQ/DX 抗体 (CR3/43) Alexa Fluor® 790

sc-53302 AF790
200 µg/ml
RMB2685.00

Is the epitope on the alpha or beta chain?

Asked by: Inquiry
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-18

Are you able to provide the approximate peptide sequence used to raise this antibody, and the epitope (C-terminus or N-terminus)?

Asked by: Katie H
Please contact Technical Service for details on this antibody epitope.
Answered by: Tech Sales
Date published: 2021-05-12

Does the antibody bind to all three MHC class II antigens, HLA-DR, HLA-DQ, HLA-DX?

Asked by: thor1
Thank you for your question. HLA-DR/DP/DQ/DX Antibody (CR3/43): sc-53302 is recommended for detection of HLA-DR/DP/DQ/DX of human origin by WB, IP, IF and IHC(P).
Answered by: Tech Support Europe
Date published: 2020-12-28

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 5 out of 5 by from Beautiful staining!This antibody is amazing for WB and IHC. Specific staining on paraffin sections and nice strong bands in the Western blot. Smart choice!
Date published: 2017-01-12
Rated 5 out of 5 by from Excellent immunoperoxidase membrane stainingExcellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Strong band in WB in GAStrong band in WB in GA-10 whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Worked well for WB in Ramos whole cell lysateWorked well for WB in Ramos whole cell lysate. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Very robust band detected in WB in RajiVery robust band detected in WB in Raji, Daudi, U-698-M and NAMALWA whole cell lysates. -SCBT QC
Date published: 2015-01-03
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HLA-DR/DP/DQ/DX 抗体 (CR3/43) is rated 5.0 out of 5 by 5.
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