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HLA-DR Antibody (Bra30) - Western Blotting - Image 399151
HLA-DR 抗体 (Bra30): sc-33717. BJAB (A), Raji (B), U-698-M (C), GA-10 (D) 和 IB4 (E) 全细胞裂解液中 HLA-DR 表达的 Western 印迹分析. 所用检测试剂: m-IgG2a BP-HRP: sc-542731.
HLA-DR 抗体 (Bra30): sc-33717. BJAB (A), Raji (B), U-698-M (C), GA-10 (D) 和 IB4 (E) 全细胞裂解液中 HLA-DR 表达的 Western 印迹分析. 所用检测试剂: m-IgG2a BP-HRP: sc-542731.

HLA-DR 抗体 (Bra30): sc-33717

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说明书
  • HLA-DR抗体(Bra30)是小鼠单克隆IgG2a (kappa轻链)，规格为200 µg/ml
  • 针对Reh-6细胞（非T、非B白血病细胞系）
  • 推荐用于 mouse, rat 和 human 来源的HLA-DR WB, IP 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 HLA-DR (520B): sc-69673，可查看有标记的 HLA-DR 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是HLA-DR Antibody (Bra30) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HLA-DR Antibody (Bra30) 打包提供（请参阅下面的订购信息）。

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    描述
    说明书与实验方案
    研究信息

    関連項目

    额外HLA抗体，包括HLA-A, HLA-A2, HLA-B, HLA-B27, HLA-B7, HLA-C, HLA class I, HLA-D, HLA-DM, HLA-DM α, HLA-DM β, HLA-DO β, HLA-DP, HLA-DP α 1, HLA-DP β 1和HLA-DQ

    主要组织相容性复合体（MHC）II类分子向CD4+辅助T细胞的呈递取决于两个关键事件。这些事件包括通过MHC分子HLA-DM和-DO的活性，使II类相关不变链肽（CLIP）从MHC II α/β二聚体的抗原结合槽中解离，以及随后的肽抗原结合。HLA-DM、-DO分子在内体/溶酶体小室和B细胞表面积累，通过维持有利于肽交换的构象来调节CLIP的解离和外源肽对HLAII类分子（HLA-DR、-DQ和-DP）的结合。对类风湿关节炎（RA）患者的HLA-DM基因进行RFLP分析表明，某些多态性是RA易感性的遗传因素。HLA-B属于HLA I 类重链同源基因。I类分子通过呈现来自内质网腔内的肽段，在免疫系统中发挥着核心作用。HLA-B和-C可以形成由膜锚定的重链和轻链（β-2-微球蛋白）组成的异二聚体。多态性产生数百种HLA-B和-C等位基因。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    HLA-DR 抗体 (Bra30)

    sc-33717
    200 µg/ml
    		RMB2377.00

    HLA-DR (Bra30): m-IgG Fc BP-HRP 套装

    sc-536783
    200 µg Ab; 10 µg BP
    		RMB2662.00

    HLA-DR (Bra30): m-IgGκ BP-HRP 套装

    sc-533947
    200 µg Ab; 40 µg BP
    		RMB2662.00

    HLA-DR (Bra30): m-IgG2a BP-HRP 套装

    sc-547870
    200 µg Ab; 10 µg BP
    		RMB2662.00

    HLA-DR 抗体 (Bra30) FITC

    sc-33717 FITC
    200 µg/ml
    		RMB2482.00

    HLA-DR 抗体 (Bra30) PE

    sc-33717 PE
    200 µg/ml
    		RMB2580.00

    SC-33720 is an anti-HLA-DR antibody that can be used in WB detection of human HLA-DR. According to SC, this antibody can also be used to detect mouse HLA-DR (usually called I-A) by WB. How was WB reactivity against mouse determined?

    Asked by: JohnK
    Thank you for your question. Reactivity with mouse is based on Western blot positive data in IB4 whole cell lysate.
    Answered by: Technical Support
    Date published: 2019-04-13

    How can HLA-DR (Bra30): sc-33717 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

    Asked by: jenniferc
    In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-33717 PE) or fluoroscein (sc-33717 FITC).
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Good specificity in WB in human HLAGood specificity in WB in human HLA-DR 3 transfected 293T whole cell lysate. -SCBT QC
    Date published: 2015-02-20
    Rated 4 out of 5 by from Very strong band in WB in BJAB whole cell lysateVery strong band in WB in BJAB whole cell lysate. -SCBT QC
    Date published: 2015-02-10
    Rated 4 out of 5 by from Robust FCM data of Raji cellsRobust FCM data of Raji cells. -SCBT QC
    Date published: 2014-08-18
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    HLA-DR 抗体 (Bra30) is rated 4.0 out of 5 by 3.
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