Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Histone H4 Antibody (F-9) - Western Blotting - Image 375340
组蛋白 H4 抗体 (F-9): sc-25260. HeLa (A), SK-N-MC (B) 和 HEK293T (C) 全细胞裂解液中组蛋白 H4 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
组蛋白 H4 抗体 (F-9): sc-25260. HeLa (A), SK-N-MC (B) 和 HEK293T (C) 全细胞裂解液中组蛋白 H4 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Histone H4 抗体 (F-9): sc-25260

3.4(9)
寫評論提問

说明书
  • Histone H4抗体(F-9)是小鼠单克隆IgG1 κ, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Histone H4的氨基酸7-103
  • Histone H4 抗体 (F-9) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的Histone H4; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • Histone H4抗体（F-9）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Histone H4 (F-9): sc-25260的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Histone H4 Antibody (F-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Histone H4 Antibody (F-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Histone抗体，包括Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4和Histone cluster 1 H3A

组蛋白H4抗体（F-9）是一种小鼠单克隆IgG1 kappa轻链抗体，靶向高度保守的组蛋白H4蛋白，识别人类组蛋白H4蛋白7-103之间的表位。抗组蛋白H4抗体（F-9）可与多种物种的组蛋白H4发生反应，包括人类、小鼠、大鼠、鸟类，以及其他物种，如马、犬、牛和猪，建议用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）。组蛋白H4是构成核小体（真核细胞染色质结构的基本单位）所必需的四种核心组蛋白（H2A、H2B、H3和H4）之一。组蛋白H4与其他核心组蛋白一起将DNA包裹成核小体颗粒，促进染色体纤维的组织和基因表达的调节。组蛋白H4蛋白会经历多种翻译后修饰，包括甲基化、乙酰化、磷酸化以及泛素化，这些修饰对于调控染色质动态、DNA修复和转录至关重要。组蛋白H4（F-9）抗体有结合和非结合两种形式，适用于各种实验室应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone H4 抗体 (F-9) 参考文献:

  1. 蛋白质图案会读取组蛋白密码吗？  |  de la Cruz, X., et al. 2005. Bioessays. 27: 164-75. PMID: 15666348
  2. 染色质中组蛋白 H1 功能的动态变化。  |  Bustin, M., et al. 2005. Mol Cell. 17: 617-20. PMID: 15749012
  3. 组蛋白 H3 的诱导性共价翻译后修饰。  |  Bode, AM. and Dong, Z. 2005. Sci STKE. 2005: re4. PMID: 15855410
  4. 组蛋白合成和核小体组装的调控。  |  Gunjan, A., et al. 2005. Biochimie. 87: 625-35. PMID: 15989979
  5. 组蛋白赖氨酸甲基化的多种功能。  |  Martin, C. and Zhang, Y. 2005. Nat Rev Mol Cell Biol. 6: 838-49. PMID: 16261189
  6. 组蛋白 H1 的基因调控：与 DNA 甲基化的新联系。  |  Rupp, RA. and Becker, PB. 2005. Cell. 123: 1178-9. PMID: 16377556
  7. 组蛋白翻译后修饰和对 DNA 双链断裂的反应  |  Wurtele, H. and Verreault, A. 2006. Curr Opin Cell Biol. 18: 137-44. PMID: 16487697
  8. 组蛋白修饰：信号受体和遗传表观遗传密码的潜在要素。  |  Nightingale, KP., et al. 2006. Curr Opin Genet Dev. 16: 125-36. PMID: 16503131
  9. 组蛋白 H3 变体及其在哺乳动物基因组索引中的潜在作用：'H3 条形码假说'。  |  Hake, SB. and Allis, CD. 2006. Proc Natl Acad Sci U S A. 103: 6428-35. PMID: 16571659
  10. 外源组蛋白H4通过诱导溶细胞死亡来协调慢性炎症。  |  Silvestre-Roig, C., et al. 2019. Nature. 569: 236-240. PMID: 31043745
  11. Hda1C在活性编码区域对组蛋白H4进行特异性脱乙酰化。  |  Lee, MK. and Kim, T. 2020. Mol Cells. 43: 841-847. PMID: 32913143
  12. MSL1通过驱动STAT3和组蛋白H4的相分离并增强其乙酰化来促进肝脏再生。  |  He, Y., et al. 2023. Adv Sci (Weinh). 10: e2301094. PMID: 37279389

订购信息

产品名称产品编号规格价格数量收藏夹

Histone H4 抗体 (F-9)

sc-25260
200 µg/ml
RMB2377.00

Histone H4 (F-9): m-IgG Fc BP-HRP 套装

sc-528387
200 µg Ab; 10 µg BP
RMB2662.00

Histone H4 (F-9): m-IgGκ BP-HRP 套装

sc-520753
200 µg Ab, 40 µg BP
RMB2662.00

Histone H4 (F-9): m-IgG1 BP-HRP 套装

sc-542911
200 µg Ab; 20 µg BP
RMB2662.00

Histone H4 抗体 (F-9) AC

sc-25260 AC
500 µg/ml, 25% agarose
RMB3129.00

Histone H4 抗体 (F-9) FITC

sc-25260 FITC
200 µg/ml
RMB2482.00

Histone H4 抗体 (F-9) PE

sc-25260 PE
200 µg/ml
RMB2580.00

Histone H4 抗体 (F-9) Alexa Fluor® 488

sc-25260 AF488
200 µg/ml
RMB2685.00

Histone H4 抗体 (F-9) Alexa Fluor® 546

sc-25260 AF546
200 µg/ml
RMB2685.00

Histone H4 抗体 (F-9) Alexa Fluor® 594

sc-25260 AF594
200 µg/ml
RMB2685.00

Histone H4 抗体 (F-9) Alexa Fluor® 647

sc-25260 AF647
200 µg/ml
RMB2685.00

Histone H4 抗体 (F-9) Alexa Fluor® 680

sc-25260 AF680
200 µg/ml
RMB2685.00

Histone H4 抗体 (F-9) Alexa Fluor® 790

sc-25260 AF790
200 µg/ml
RMB2685.00

What size band do you expect to with these antibodies? The representative gel shows around 37kDa

Asked by: Shh133
Thank you for your question. According to the datasheet of Histone H4 Antibody (F-9): sc-25260 there are three expected molecular weights for different modifications of Histone H4: Molecular Weight of acetylated Histone H4: 11 kDa Molecular Weight of non-acetylated Histone H4: 11 kDa Molecular Weight of hyper-acetylated Histone H4: 35 kDa The antibody is specific against protein with any of those modifications.
Answered by: Tech Support Europe
Date published: 2020-09-28

Does this antibody also work with Wes technology (SimpleWestern)?

Asked by: KSRe
Thank you for your question. Histone H4 Antibody (F-9): sc-25260 has been tested in manual western blots only, but should also work in automated systems.
Answered by: Tech Support Europe
Date published: 2020-09-01

Buongiorno, Histone H4 Antibody (F-9) sc-25260 200 µg/ml €285.00 285 euro è il prezzo per quanti microlitri di prodotto? Grazie

Asked by: paolam
Molte grazie per la sua domanda. Il sc-25260 contiene 200 g IgG1 in 1.0 ml di PBS con < 0.1% azoturo di sodio azide e 0.1% gelatina. Contatti il nostro servizio tecnico tramite telefono, email o live chat se necessita di ulteriori informazioni. Thank you for your question. sc-130349 contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Please contact our Technical Service by phone or email or by Live Chat if you need additional information.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-20
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_25260, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Wouldn't Recommend itI have tested this antibody with multiple samples from mammalian cell lines, both with total cellular material as well as purified nuclei, but I couldn 't get any band whatsoever.
Date published: 2020-01-15
Rated 3 out of 5 by from okAntibody was tested in rat liver and rat muscle samples. Dilution 1:100. 2 week bands were detected.
Date published: 2018-09-05
Rated 1 out of 5 by from WB on histone extractsNot very specific, Can bind to all four core histones
Date published: 2017-10-03
Rated 1 out of 5 by from Not good for IFNot working for IF in hunam HT1080 cells at 1:100 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Superb WBVery clear western blot data; strong signal, low background
Date published: 2017-02-05
Rated 5 out of 5 by from Looking for a Histone H4 antibody for WB?If so, this is the one! It's nice and strong, reliable and consistent, a great choice!
Date published: 2016-12-30
Rated 5 out of 5 by from Good antibody for western blot analysis ofGood antibody for western blot analysis of acetylated Histone H4 expression in SK-N-MC whole cell lysate. - SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Performs well in western blot analysis ofPerforms well in western blot analysis of acetylated Histone H4 expression in non-transfected 293T, human Histone H4 transfected 293T and HeLa whole cell lysates. - SCBT QC
Date published: 2015-04-01
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_25260, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 12ms
  • REVIEWS, PRODUCT
Histone H4 抗体 (F-9) is rated 3.4 out of 5 by 9.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_25260, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 124ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。