Date published: 2025-9-6
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Histone H1 Antibody (H-2) - Western Blotting - Image 294775
组蛋白 H1 抗体 (H-2): sc-393358. A-431 (A), LNCaP (B), HeLa (C) 和 HL-60 (D) 全细胞裂解液及 Jurkat 核提取物 (E) 中组蛋白 H1 表达的 Western 印迹分析.
组蛋白 H1 抗体 (H-2): sc-393358. A-431 (A), LNCaP (B), HeLa (C) 和 HL-60 (D) 全细胞裂解液及 Jurkat 核提取物 (E) 中组蛋白 H1 表达的 Western 印迹分析.

Histone H1 抗体 (H-2): sc-393358

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说明书
  • Histone H1抗体(H-2)是小鼠单克隆IgG1 λ Histone H1抗体, 在27篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Histone H1的N-terminus附近的氨基酸22-47之间
  • Histone H1 抗体 (H-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 all histone H1 isoforms
  • 抗Histone H1抗体（H-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-393358 X, 200 µg/0.1 ml)
  • 关于如何获取Histone H1 (H-2): sc-393358的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP（小鼠 IgGλ 结合蛋白-HRP）是Histone H1 Antibody (H-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Histone H1 Antibody (H-2) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGλ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Histone抗体，包括Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4和Histone cluster 1 H3A

Histone H1 Antibody (H-2) 是一种 IgG1 λ 小鼠单克隆 Histone H1 抗体（也称为 H1-4 抗体、Histone H1.4 抗体或 Histone H1b 抗体），可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类的 Histone H1 蛋白。Histone H1 Antibody (H-2) 既有非偶联形式的抗 Histone H1 抗体，也有多种偶联形式的抗 Histone H1 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。真核细胞的组蛋白是核内的基础性水溶性蛋白，它们通过连续地将 146 个碱基对的 DNA 包裹在左旋超螺旋中形成核小体粒子。八聚体由四种核心组蛋白（H2A、H2B、H3 和 H4）的两个分子组成；由两个 H2A-H2B 二聚体和两个 H3-H4 二聚体组成，通过三级结构形成两个近似对称的半部分。超过 80% 的核小体含有来自无内含子基因的连接组蛋白 H1，它与核小体之间的连接 DNA 相互作用，从而促进形成更高级别的染色质。组蛋白受到酶的后翻译修饰，主要是在其 N 端尾部，也可以在其球状结构域上。这些修饰包括甲基化、柠檬酸化、乙酰化、磷酸化、增稠酶化、泛素化和 ADP-核糖基化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Histone H1 抗体 (H-2)

sc-393358
200 µg/ml
RMB2377.00

Histone H1 (H-2): m-IgGλ BP-HRP 套装

sc-523916
200 µg Ab, 40 µg BP
RMB2662.00

Histone H1 抗体 (H-2) X

sc-393358 X
200 µg/0.1 ml
RMB2377.00

Histone H1 抗体 (H-2) AC

sc-393358 AC
500 µg/ml, 25% agarose
RMB3129.00

Histone H1 抗体 (H-2) HRP

sc-393358 HRP
200 µg/ml
RMB2377.00

Histone H1 抗体 (H-2) FITC

sc-393358 FITC
200 µg/ml
RMB2482.00

Histone H1 抗体 (H-2) PE

sc-393358 PE
200 µg/ml
RMB2580.00

Histone H1 抗体 (H-2) Alexa Fluor® 488

sc-393358 AF488
200 µg/ml
RMB2685.00

Histone H1 抗体 (H-2) Alexa Fluor® 546

sc-393358 AF546
200 µg/ml
RMB2685.00

Histone H1 抗体 (H-2) Alexa Fluor® 594

sc-393358 AF594
200 µg/ml
RMB2685.00

Histone H1 抗体 (H-2) Alexa Fluor® 647

sc-393358 AF647
200 µg/ml
RMB2685.00

Histone H1 抗体 (H-2) Alexa Fluor® 680

sc-393358 AF680
200 µg/ml
RMB2685.00

Histone H1 抗体 (H-2) Alexa Fluor® 790

sc-393358 AF790
200 µg/ml
RMB2685.00

Histone H1 (H-2) 中和勝肽

sc-393358 P
100 µg/0.5 ml
RMB511.00

What is the recommended range of concentration of the antibody sc393358 for western blot?

Asked by: Manali Dey
Thank you for your question. We recommend a dilution range of 1:100-1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-01-11

When should the TransCruz reagent sc-393358 X be used instead of the standard form sc-393358?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 7
Date published: 2017-01-13

What secondary is best to use with Histone H1 (H-2): sc-393358?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Histone H1 (H-2):sc-393358, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from Works goodClean and strongs bands at 34 kDa. I loaded only 20 ug and got clear bands.
Date published: 2024-02-07
Rated 1 out of 5 by from Not good for IFWeak signal for IF in hunam HT1080 cells at 1:100 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good for WBStrong single band - worked well in nuclear fractionation WB.
Date published: 2017-09-13
Rated 5 out of 5 by from Works great for Western Blot.Used at a concentration of 1:2000 for Western blot with mouse tissues. Worked very well, clean blot and a single band. I would recommend!
Date published: 2017-09-01
Rated 5 out of 5 by from Superb !!Great product for western blot applications; strong signal
Date published: 2017-01-20
Rated 5 out of 5 by from good in WBThis Histone H1 antibody shows specific strong bands in WB.
Date published: 2017-01-12
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~32-33 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using A-431, LNCaP, HeLa and HL-60 whole cell lysates and Jurkat nuclear extract. -SCBT QC
Date published: 2014-07-05
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Histone H1 抗体 (H-2) is rated 4.4 out of 5 by 8.
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